Environmental conservation is an important issue that should be instilled in students through education, as it plays a vital role in shaping environmentally responsible attitudes and behaviors. Adequate environmental knowledge is expected to enhance students' concern for environmental conservation. This study aimed to analyze the relationship between students' environmental knowledge and their concern for environmental conservation at SMA Negeri 16 Makassar. The study employed a quantitative approach with a correlational research design. The population consisted of 216 eleventh-grade students, while a sample of 60 students was selected using simple random sampling. Data were collected through an environmental knowledge test and an environmental concern questionnaire, both of which were validated through expert judgment. Data were analyzed using descriptive and inferential statistics, with the Pearson Product-Moment correlation test conducted using IBM SPSS Statistics version 29. The findings revealed that the mean score of students' environmental knowledge was 61.20, while the mean score of environmental concern was 51.20, both categorized as moderate. The Pearson correlation analysis indicated a strong and statistically significant positive relationship between environmental knowledge and students' concern for environmental conservation (r = 0.754, p < 0.001). These findings suggest that higher levels of environmental knowledge are associated with greater concern for environmental conservation among students. Therefore, strengthening environmental education through contextual learning and the continuous promotion of environmentally friendly practices in schools is essential to foster students' environmental concern.