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Potential Analysis of Lemna sp. Extract as Immunostimulant to Increase Non-Specific Immune Response of Tilapia (Oreochromis niloticus) against Aeromonas hydrophila Armando, Eric; Lestiyani, Ayu; Islamy, R. Adharyan
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.6

Abstract

Lemna sp. is known to have several bioactive compounds and polysaccharide macromolecules that can function as immunomodulators to affect non-specific immune responses to increase the body's resistance to pathogens. This study aims to determine the potential of catfish eye extract as an immunostimulant by observing non-specific tilapia immune parameters. The extraction method used was 96% ethanol maceration for 2 days with a ratio of 1: 4. The experimental design used a Completely Randomized Design with 5 treatments (doses 0.2, 0.4, 0.6 mg/kg, control + and control -) and 3 replications. The non-specific parameters of immunity observed included total plasma protein (Bradford method), superoxide dismutase and lysozyme activity. The data obtained will be analyzed using ANOVA, if there is a significant difference, it will be further tested with Duncan Multiple. Range Test (DMRT). The results showed that the highest total plasma protein was found in treatment C (giving an extract of 0.3 mg/kg body weight) with an average total plasma protein after 12 days of maintenance of 4.99 g / dL. The extract dose of 0.3 mg/body weight showed a rapid decrease in SOD and increase Lysozyme activity.
NUTRITIONAL AND CAROTENOID CHARACTERIZATION OF YELLOW PUMPKIN (Cucurbita moschata) POWDER FOR POTENTIAL USE IN ORNAMENTAL FISH FEED Islamy, R. Adharyan; Faqih, Abd Rahem; Putra, Arya Darmawan Kurniadi; Ramadhani, Auryn Novia; Czech, Michael
Journal of Aquatropica Asia Vol 10 No 1 (2025): Journal of Aquatropica Asia
Publisher : Program Studi Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v10i1.6363

Abstract

Yellow pumpkin (Cucurbita moschata) is a natural plant source rich in carotenoids, particularly β-carotene, with promising potential as a functional ingredient in ornamental fish diets. This study aimed to evaluate the nutritional composition and carotenoid profile of yellow pumpkin powder as a preliminary step toward its application in enhancing fish coloration. Proximate analysis revealed high carbohydrate content (61.25%), crude fiber (10.48%), and moderate protein levels (13.26%). Furthermore, the β-carotene content reached 102.4 mg/kg, highlighting its value as a natural pigment source. Essential minerals such as potassium, calcium, and magnesium were also present in considerable amounts. These findings suggest that yellow pumpkin powder is a nutritious, sustainable, and pigment-rich feed ingredient suitable for use in ornamental aquaculture.
PHYTOCHEMICAL CHARACTERIZATION AND NUTRITIONAL EVALUATION OF Ficus septica FOR AQUAFEED DEVELOPMENT IN HERBIVOROUS FISH: KARAKTERISASI FITOKIMIA DAN EVALUASI NUTRISI Ficus septica UNTUK PENGEMBANGAN PAKAN AKUATIK PADA IKAN HERBIVORA Islamy, R. Adharyan; Setyowati, Najwa Lutfi; Ni'matullah, Syakir
Journal of Aquatropica Asia Vol 10 No 2 (2025): Journal of Aquatropica Asia
Publisher : Program Studi Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/910n0030

Abstract

The sustainability of aquaculture relies heavily on the development of nutritionally balanced and cost-effective feed alternatives that reduce dependency on conventional protein sources. F. septica, a medicinal plant rich in bioactive compounds, offers potential as a novel functional feed ingredient for herbivorous fish. This study aimed to characterize the phytochemical profile and evaluate the proximate nutritional composition of F. septica leaves, and to assess their effects on growth performance and feed utilization in herbivorous fish. Phytochemical screening revealed high levels of phenolics (33.5 ± 2.1 mg/g), flavonoids (28.7 ± 1.3 mg/g), and tannins (16.1 ± 0.9 mg/g), indicating strong antioxidant and antimicrobial potential. Proximate analysis showed that F. septica leaves contained 18.7% protein, 40.7% nitrogen-free extract, and 16.3% fiber. Feeding trials with diets containing 0% (control), 5%, and 10% F. septica leaf powder demonstrated significant improvements in weight gain, specific growth rate, and feed conversion ratio in the 5% and 10% groups (p < 0.05), without compromising survival. These results suggest that F. septica can be effectively utilized as a nutrient-rich, bioactive aquafeed additive for herbivorous fish. Further research on digestibility, long-term effects, and residue safety is recommended to validate its practical applications in commercial aquaculture.