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Produksi Propolis dari Lebah Tetragonula laeviceps Menggunakan Sarang MOTIVE yang Dilengkapi dengan Sistem Instrumentasi Hakim, Muhammad Naufal; Abduh, Muhammad Yusuf
Jurnal Otomasi Kontrol dan Instrumentasi Vol 10 No 2 (2018): Jurnal Otomasi Kontrol dan Instrumentasi
Publisher : Pusat Teknologi Instrumentasi dan Otomasi (PTIO) - Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/joki.2018.10.2.6

Abstract

Penelitian yang dilakukan bertujuan untuk merancang dan mengimplementasikan sistem instrumentasi pada sarang lebah yang diberi nama MOTIVE untuk budidaya lebah Tetragonula laeviceps. Tujuan implementasi ini adalah untuk mengoptimalkan produksi propolis berdasarkan data dari sistem instrumentasi menuju sistem pertanian presisi berbasis data. Bahasa program yang digunakan sebagai antarmuka pada Arduino dan Raspberry Pi adalah Arduino dan Python. Parameter yang diukur oleh sensor pada sarang MOTIVE adalah temperatur, kelembapan, massa sarang MOTIVE serta total lebah keluar masuk sarang yang diperbarui setiap 15 dan 30 menit sekali. Variasi penelitian berupa intensitas cahaya yang diimplementasikan pada koloni dalam sarang bambu, sarang MOTIVE berbahan kayu, dan sarang MOTIVE berbahan akrilik. Propolis yang dihasilkan oleh lebah dipanen untuk dianalisis kandungan flavonoid berbasis ekivalen kuersetin. Data yang diperoleh menunjukkan bahwa produktivitas propolis yang dihasilkan oleh lebah pada sarang MOTIVE sebanyak 2-2,4 g/sarang/minggu. Nilai kandungan flavonoid tertinggi pada ekstrak propolis diperoleh dari propolis yang dihasilkan oleh lebah pada sarang MOTIVE kayu sebanyak 14,3 mg QE/gram. Sistem instrumentasi terbukti berhasil melakukan perekaman data secara simultan dan tidak mengganggu aktivitas lebah. Berdasarkan parameter yang diukur, konversi resin menjadi propolis secara optimal terjadi pada sarang dengan variansi temperatur dan kelembapan yang kecil, serta intensitas cahaya pada sarang terbukti berpengaruh pada produksi propolis.
INFLUENCE OF WATER STRESS AND PLANT AGE ON THE YIELD AND CHEMICAL COMPOSITION OF ESSENTIAL OIL FROM Cymbopogon winterianus Jowitt Abduh, Muhammad Yusuf; Ibarahim, Izni Nur; Atika, Ulya; Manurung, Robert; Suheryadi, Dedi
BIOTROPIA Vol. 27 No. 1 (2020): BIOTROPIA Vol. 27 No. 1 April 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2020.27.1.1152

Abstract

Citronella oil, which is also known as Java citronella oil, is typically obtained from the leaves of Cymbopogon winterianus Jowitt and has been widely used as an antiseptic, fragrance, flavor, and many other applications. However, systematic studies on the factors affecting the yield and composition of citronella oil are limited. Hence, this study was carried out to investigate the influence of water stress (low to high) and plant age (0.5 to 5 years) on the yield and composition of citronella oil. Citronella leaves were dried at room temperature (26–30 °C) with a relative humidity of 62–74% for 3 days before the citronella oil was extracted using a steam distillation unit. Introducing a high water stress condition to the plants increased the yield from 0.80% (control) to 1.4% (high water stress) dry weight. The geraniol content slightly increased from 15% to 17%, whereas the citronellal content considerably increased from 31% to 44% when the plants were subjected to high water stress in comparison to the control. The oil yield increased with plant age before it reached its plateau at 3.5% dry weight at age 4 years. However, the composition of citronella oil did not significantly vary with plant age.
Effect of Pectin Biodegradation with Aspergillus niger on Total Flavonoid Content of Citrus limon L.) Abduh, Muhammad Yusuf; Zuliansyah, Wahyu; Aprina, Lestari; Arazella, Natalie
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4537

Abstract

Lemon peel and leaf  as well as lower grade of lemon contain a considerable amount of flavonoid which can be extracted to produce a valuable product. The presence of pectin in lemon hinders the extraction of flavonoid compound. This research was conducted to examine the effect of pectin biodegradation of lemon leaf, peel, and lower grade fruit using solid-state fermentation towards the yield of pectin, crude flavonoid, and total flavonoid content. Fermentation was carried out with the help of Aspergillus niger at 30℃ for 3, 5, 7, and 9 days of cultivation time. Pectin was extracted from the fermentation broth using citric acid. Crude flavonoid was extracted using maceration and stirred about 500 rpm at 50℃ for 40 minutes. Total flavonoid content was analyzed using a spectrophotometer. The optimum cultivation time of Aspergillus niger with leaf and peel was 9 days with a pectin yield from lemon leaf was 0.43% dry weight and the pectin yield from lemon peel was 0.04% dry weight. As for the lower grade fruit, a cultivation time for 7 days showed an optimal result with a pectin yield of 0.08% dry weight. The total flavonoid content in the leaf increased up to 94.3% (1.06 mg of the quercetin equivalents per g of substrate) whereas the total flavonoid content in the peel and lower grade fruit increased up to 42% (0.12 mg of the quercetin equivalents per g of substrate) and 48% (0.021 mg of the quercetin equivalents per g of substrate), respectively. These results highlighted the potential valorization of Citrus limon L. as an alternative source for producing flavonoid in food and pharmaceutical industry. Â