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All Journal AGRIVITA, Journal of Agricultural Science Biosaintifika: Journal of Biology & Biology Education
Oktavia, Gebby Agnessya Esa
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Agriculture, Biological Sciences & Forestry

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Plant Diversity and Composition of Three Different Altitudes in Sikka Forest Area - East Nusa Tenggara Fiqa, Abban Putri; Oktavia, Gebby Agnessya Esa
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23565

Abstract

East Nusa Tenggara located in the Wallacea area that has many endemic species. However, the existence of germplasm diversity in this area has been threatened due to landscape changes and deforestation. The research was conducted to identify floristic composition in Sikka forest area. This research also aimed to compare that plant composition in Sikka forest area on each of the different altitudes, including their diversity richness and environmental factors. Two sampling methods were used, which are Point-Centered Quarter (PCQ) method that applied for trees and nested sampling method 5x5 m2 for sapling and 2x2 m2 for ground cover. Measured parameters were Relative Density (RD), Relative Frequency (RF), Relative Coverage/dominance (RC), Important Value Index (IVI), Diversity Index (H'), and Jaccard Similarity Index (J’). Research results showed that the diversity index of each region was relatively low. Plant composition of tree, sapling, and groundcover in three different altitudes was quite different. This result indicates that the plant composition in the Sikka, East Nusa Tenggara is very diverse. The temperature and relative humidity in each altitude area observed was significantly different. One of the endemic species of Flores Island, Eucalyptus urophylla, was only found at 500-700 m asl. There are very limited research on the vegetation diversity held in Flores, East Nusa Tenggara. This research can be the basic for further research in order to explore the potential diversity of flora in this area.
Amorphophallus muelleri Blume Shoot Induction on Different Media Types and Plant Growth Regulator Combinations Oktavia, Gebby Agnessya Esa; Junairiah, Junairiah; Manuhara, Yosephine Sri Wulan
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.4477

Abstract

Conventional propagation experiences problems due to long period for seed production, disease contamination, low reproductive rate and season dependent. In vitro propagation offers several advantages. This research aims to assess the impact of different media types and the concentrations of BAP and IBA on the in vitro growth and development of A. muelleri shoots. Bulbil explants were cultivated using MS media with different media types (solid media, agitated liquid media, and static liquid media) with various BAP and IBA concentration treatments (1 mg/l, 2 mg/l, and 3 mg/l). The results demonstrated that the media type and combinations of BAP and IBA treatments significantly influenced period of callus formation and the number of shoots. The development of explant on both solid and static liquid media involves initial explant swelling, formation of nodular callus, greenish buds, and ultimately shoots. Anatomical observation showed that explants experienced indirect organogenesis, characterized by the presence of pre meristem nodular callus, meristematic tissue, apical meristem and leaf primordia. The agitated liquid media didn’t promote growth and development of the explants. The most effective combination was found to be the static liquid media supplemented with 3 mg/l BAP and 1 mg/l IBA.
Co-Authors Fiqa, Abban Putri Junairiah Junairiah Yosephine Sri Wulan Manuhara