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Callus Induction of Piper betle L. Var Nigra Using 2,4-Dichlorofenoxyacetic Acidand 6-Benzil Aminopurin Junairiah, Junairiah; Purnomo, Purnomo; Utami, Edy Setiti Wida; Ni’matuzahroh, Ni’matuzahroh; Sulistyorini, Lilis
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.143 KB) | DOI: 10.15294/biosaintifika.v10i3.15962

Abstract

Piper betle L var Nigra (black betel) is a member of Piperaceae family which has potential as medicinal plant due to its secondary metabolites. Callus culture is one of the alternative methods to elevate production of secondary metabolites. This study was aimed to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzil aminopurine (BAP) towards callus induction and growth, also to determine the most optimal variation of 2,4-D and BAP concentration for callus induction of black betel leaf explant. This study was a laboratory experimental study with complete randomized design. Black betel leaf explant was planted in Murashige and Skoog (MS) medium supplemented with 2,4-D and BAP growth regulators at concentration of 0.0 mg/L, 0.5 mg/L, 1.0 mg/L, 1.5 mg/L, 2.0 mg/L respectively. Parameter recorded for callus induction and growth including callus induction time (days), percentage of explant forming callus, fresh weight, dry weight, color and texture. After callus planted for 8 weeks, analysis was performed statistically. Result showed that 2,4-D and BAP supplementation to medium affected the growth of black bete l leaf explants. Additional concentration of 0.5 mg/L 2,4-D and 1.0 mg/L BAP growth regulators showed the fastest response in callus formation, at 7.25 days. Growth regulators of 2,4-D 0.5 mg/L and BAP 2.0 mg/L concentration produced the highest fresh and dry weight, at 0.6802 g and 0.0670 g respectively. The best treatment was used as a basis to produce secondary metabolites.
IDENTIFIKASI METABOLIT SEKUNDER DAN AKTIVITAS ANTIMIKROB EKSTRAK ETIL ASETAT DUMORTIERA HIRSUTA Junairiah, Junairiah; Sa'diyah, Muhimmatus; Salamun, Salamun
Sains & Matematika Vol 3, No 2 (2015): April, Sains & Matematika
Publisher : Sains & Matematika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Salah satu keanekaragaman flora di Indonesia adalah Dumortiera hirsuta. Tumbuhan ini berpotensi sebagai bahan antibakteri dan antifungi. Penelitian ini bertujuan untuk mengidentifi kasi dan menguji aktivitas antimikrob metabolit sekunder ekstrak etil asetat D. hirsuta. Metabolit sekunder diidentifi kasi dengan skrining fitokimia. Aktivitas antimikrob dilakukan dengan uji difusi dan uji dilusi terhadap Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Candida albicans ATCC 10231. Penelitian terdiri atas 24 perlakuan dan masing-masing terdiri atas tiga ulangan. Data yang diperoleh berupa diameter zona hambat, minimal inhibitary concentration (MIC), minimal bacterisidal/fungisidal concentration (MBC/MFC). Data dianalisis secara deskriptif. Berdasarkan hasil identifi kasi, diketahui bahwa ekstrak etil asetat D hirsuta mengandung flavonoid, alkaloid, steroid dan dapat menghambat mikrob patogen. One of the diversity of flora in Indonesia is Dumortiera hirsuta. This plant has the potential as an antibacterial and antifungal. This study aimed to identify and test the antimicrobial activity of secondary metabolites ethyl acetate extract of D. hirsuta. Secondary metabolites were identified by phytochemical screening. Antimicrobial activity performed by diffusion test and dilution test against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25 922 and Candida albicans ATCC 10231. This research consisted of 24 treatments and each consists of three replications. The obtained data were the diameter of inhibition zone, minimal inhibitory concentration (MIC), minimal bactericidal/fungicidal concentration (MBC/MFC). Data were analyzed descriptively. The identifi cation result showed that the ethylacetate extract of D hirsuta contained flavonoids, alkaloids, steroid, and can inhibit pathogen microbes.
Callus induction and secondary metabolite profile from Elephantopus scaber L. Junairiah Junairiah; Diah Ayu Wulandari; Edy Setiti Wida Utami; Nabilah Istighfari Zuraidassanaaz
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59234

Abstract

Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol.  Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.
ISOLATION OF BIOACTIVE COMPOUNDS FROM DICRANACEAE MOSSES Junairiah Junairiah; Tri Nurhariyati; Ni'matuzahroh Ni'matuzahroh; Lilis Sulistyorini
Jurnal Kimia Riset Vol. 1 No. 2 (2016): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1205.878 KB) | DOI: 10.20473/jkr.v1i2.3086

Abstract

ABSTRACT            Dicranoloma reflexum and Dicranella coarctata are mosses from Dicranaceae family. This study was purposed to identify bioactive compounds contained from both species. Dicranoloma reflexum and Dicranella coarctata collected form Cangar forest, Batu, East Java. Mosses was rinsed, dried and crushed into powder. Extraction was performed using maceration method with n-hexane, acetic acid, and methanol solvent. Compounds obtained then identified using Gass Chromatography Mass Spectra. Result showed that n-hexane, acetic ethyl, and methanol extract of Dicranoloma reflexum contained 61, 16, and 58 compounds respectively. Main component of each extract was 1-octadecene, phenol, and 9-octadecanoic acid. N-hexane, acetic ethyl, and methanol extract of Dicranella coarctata contained 5, 38, and 23 compounds respectively. Main component of each extract was thiosulphuric acid, E-15 heptedecenal, and n-hexadecanoic acid.Key words : Dicranaceae, bioactive compounds
Analisis GCMS terhadap Senyawa Fitokimia Ekstrak Metanol Ganoderma lucidum Surahmaida Surahmaida; Tri Puji Lestari Sudarwati; Junairiah Junairiah
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.637 KB) | DOI: 10.20473/jkr.v3i2.12060

Abstract

AbstrakPenelitian ini bertujuan untuk menganalisis senyawa fitokimia ekstrak metanol Ganoderma lucidum dengan metode GCMS. Tahapan penelitian ini meliputi  proses ekstraksi serbuk halus G. lucidum menggunakan pelarut metanol dengan metode maserasi selama 3x3 hari dan ekstrak metanol G. lucidum yang didapatkan dianalisis dengan alat GCMS. Senyawa fitokimia yang teridentifikasi oleh GCMS antara lain thiophene, 2-hexyl-; 3-((3-Acetoxythyl)-6-acetoxymethyl-2,4-dimethyl)phenyl)-2-methyl-(E)-2-propenyl acetate; 2,7-Diphenylindole; dan 5-(2-Bromophenyl)-7-chloro-2,3-dihydro-1H-1,4-benzodiazepin-2-one. Dapat disimpulkan bahwa senyawa fitokimia pada ekstrak metanol G. lucidum memiliki efek farmakologis bagi kesehatan manusia dan aktivitas biologis lainnya.  Katakunci: Ganoderma lucidum, senyawa fitokimia, GCMS AbstractThis study aims to analyze the phytochemical compounds of methanol extract of Ganoderma lucidum using the GCMS method. The stages of this study include extraction of fine powder G. lucidum using methanol solvent by maceration method for 3x3 days and methanol extract of G. lucidum obtained were analyzed by GCMS. Phytochemical compounds identified by GCMS is thiophene, 2-hexyl-; 3 - ((3-Acetoxythyl) -6-acetoxymethyl-2,4-dimethyl) phenyl) -2-methyl- (E) -2-propenyl acetate; 2,7-Diphenylindole; and 5- (2-Bromophenyl) -7-chloro-2,3-dihydro-1H-1,4-benzodiazepine-2-one. It can be concluded that phytochemical compounds in methanol extract of G. lucidum have pharmacological effects on human health and other biological activities. Keywords: Ganoderma lucidum, phytochemical compounds, GCMS
ISOLATION AND IDENTIFICATION OF SECONDARY METABOLITES OF BLACK BETEL (Piper betle L. var Nigra) Junairiah Junairiah; Ni’matuzahroh Ni’matuzahroh; Nabilah Istighfari Zuraidassanaaz; Lilis Sulistyorini
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.337 KB) | DOI: 10.20473/jkr.v3i2.12064

Abstract

AbstractPiper betle L. var Nigra commonly known as black betel have a potential as drug raw materials. The leave of black betel  can be used to resist bleeding so as to accelerate the healing of wounds on the skin, sputum, other uses are for epistaxis, as well as for dialysis, asthma, bronchitis, cough, and high blood pressure. Many uses of black betel leaves make it interesting to learn its metabolites compounds by phytochemical screening. So, this research aimed  to isolate and identificate secondary metabolites of Piper betle L. var Nigra. This research used three kind of organic solvents, there were methanol, ethyl acetate, and n-hexane. Several screening tests were used to isolate and identificate the phytochemical compound, including screening of alkaloids, terpenoids/steroids, flavonoids, polyphenols, tannins, and saponins. The results showed that methanol extract of black betel leaves contained of alkaloids, terpenoids/steroid, flavonoids, polyphenols and tannins compounds. The ethyl acetate extract of black betel leaves contained of terpenoids/steroid, flavonoids and tannins compounds. The n-hexane extract of black betel leaves contained of terpenoids/steroid. The methanol extract of Piper betle L. var Nigra contained more secondary metabolites than n-hexane and ethyl acetate extracts. Keywords : methanol ; Phytochemical screening ; Piper betle L. var Nigra.
Senyawa Bioaktif Daun Kumis Kucing (Orthosiphon stamineus) Surahmaida Surahmaida; Umarudin Umarudin; Junairiah Junairiah
Jurnal Kimia Riset Vol. 4 No. 1 (2019): Juni
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.619 KB) | DOI: 10.20473/jkr.v4i1.13176

Abstract

AbstrakDaun kumis kucing (Orthosiphon stamineus) umumnya digunakan sebagai tanaman obat keluarga, hal ini diduga karena senyawa bioaktif yang terkandung didalamnya. Tujuan penelitian ini untuk mengetahui senyawa bioaktif ekstrak n-heksana daun kumis kucing (Orthosiphon stamineus) menggunakan GCMS. Hasil senyawa bioaktif yang teridentifikasi ekstrak n-heksana daun kumis kucing mengandung 1,1-Dicyclopentylethane; Bicyclo[3.2.0]hept-2,6-diene-1,2,3,4,4,5,6-d(7); n-Butyl palmitate; 1-oxo03.alpa.-(4-methyl-3-pentenyl)-6.alpa.-methyl-6a.alpa.-carbomethoxy-1,3,3a.alpa.,6a-tetrahydrocyclopenta[c]furan;  1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethylheptasiloxane; 1,4-bis(trimethylsilyl)-benzene dan Silicone grease, Siliconfett. Senyawa-senyawa bioaktif ekstrak n-heksana daun kumis kucing tersebut kemudian dianalisis aktivitas biologi dan farmakologisnya. Katakunci: Orthosiphon stamineus, senyawa bioaktif, GCMS AbstractThe leaves of cat whiskers (Orthosiphon stamineus) are generally used as family medicinal plants, this is thought to be due to the bioactive compounds contained therein. The purpose of this study was to find out the bioactive compounds of n-hexane extract of cat whiskers (Orthosiphon stamineus) using GCMS. The results of bioactive compounds identified with n-hexane extract of cat whiskers containing 1,1-Dicyclopentylethane; Bicyclo[3.2.0]hept-2,6-diene-1,2,3,4,4,5,6-d(7); n-Butyl palmitate; 1-oxo03.alpa.-(4-methyl-3-pentenyl)-6.alpa.-methyl-6a.alpa.-carbomethoxy-1,3,3a.alpa.,6a-tetrahydrocyclopenta[c]furan;  1,1,3,3,5,5,7,7,9,9,11,11,13,13-tetradecamethylheptasiloxane; 1,4-bis(trimethylsilyl)-benzene and Silicone grease, Siliconfett. The bioactive compounds of the n-hexane extract of the cat's whiskers leaves were then analyzed for their biological and pharmacological activities. Keywords: Orthosiphon stamineus, bioactive compound, GCMS
PENGARUH VARIASI ZAT PENGATUR TUMBUH IAA, BAP, KINETIN TERHADAP METABOLIT SEKUNDER KALUS SIRIH HITAM (Piper betle L. Var Nigra) Junairiah Junairiah; Nurul Sofi Amalia; Yosephine Sri Wulan Manuhara; Ni’matuzzahroh Ni’matuzahroh; Lilis Sulistyorini
Jurnal Kimia Riset Vol. 4 No. 2 (2019): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v4i2.16898

Abstract

AbstrakPiper betle L. var Nigra (sirih hitam)  merupakan salah satu jenis tanaman obat yang berpotensi untuk dikembangkan dengan metode kultur jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh variasi zat pengatur tumbuh Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), Kinetin terhadap senyawa metabolit sekunder  yang terdapat kalus sirih hitam. Eksplan daun dari sirih hitam ditanam pada medium Murashige dan Skoog dengan 4 perlakuan kombinasi (I1,0K1,0; I1,0K1,5; I1B1,5; I0,5B0,5). Kalus dipelihara selama 8 minggu. Simplisia kalus sirih hitam di maserasi menggunakan pelarut metanol dan diidentifikasi menggunakan Gas Chromatography Mass Spectra (GCMS). Berdasarkan analisis GCMS menunjukkan bahwa senyawa metabolit sekunder dengan presentase tertinggi terdapat pada masing- masing perlakuan yaitu senyawa gamma sitosterol (14,88%) pada perlakuan I1,0K1,0 ; senyawa 14-Beta H Pregna (15,94%) pada perlakuan I1,0K1,5 serta senyawa beta-d-glucopyranoside (15,54%) pada perlakuan I0,5B0,5; dan senyawa beta-d-glucopyranoside (5,63%) pada perlakuan I1,0 B1,5. Katakunci: Kalus, Piper betle L. var Nigra, senyawa metabolit sekunder  AbstractPiper betle L. Var Nigra (black betel) is one type of medicinal plant that has the potential to be developed by tissue culture method. This study was aims to determine the effect of variations in growth regulator Indole Acetic Acid (IAA), Benzyl Amino Purine (BAP), and Kinetin on secondary metabolite compounds contained in black betel callus. The explant of black betel’s leaf was cultured on Murashige and Skoog medium with four combination treatments (I1.0K1.0; I1.0K1.5; I1B1.5; I0.5B0.5). The callus was maintained for eight weeks. Simplisia of black betel’s callus was macerated using methanol solvent and identified using Gas Chromatography Mass Spectra (GCMS). GCMS analysis showed that secondary metabolites with the highest percentage in each treatment were gamma sitosterol compound (14.88%) in treatment I1.0K1.0; 14-Beta H Pregna compound (15.94%) in I1.0K1.5 treatment and beta-d-glucopyranoside compound (15.54%) in I0.5B0.5 treatment; and beta-d-glucopyranoside compound (5.63%) in I1.0 B1.5 treatment.Keywords: Callus, Piper betle L. Var Nigra, secondary metabolite compounds
INNOVATION OF MEDICINE PLANTS PROPAGATION WITH VERTICULTURE TECHNIQUES IN EFFORTS TO OVERCOME LAND DYSFUNCTIONAL IN EAST SURABAYA AREA Junairiah Junairiah; Tri Nurhariyati; Listijani Suhargo
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 2 No. 2 (2020): DECEMBER 2020
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (751.964 KB) | DOI: 10.20473/dc.V2.I2.2020.58-61

Abstract

Background: Keputih Village is located in eastern Surabaya. The boundary of this village is the river. Areas on the river banks are overgrown with wild plants and poorly maintained. One way to overcome the dysfunctional land around the river is by cultivating medicinal plants with verticulture techniques. Purpose: The implementation of this program is to overcome the dysfunctional land around the Keputih Village’s river by cultivating medicinal plants in verticulture techniques and provide training in the manufacture of herbal products from the cultivation of medicinal plants and provide financial management training to the local residents. Methods: This activity was carried out in 4 stages. Phase I was training in making pots and verticulture shelves as well as planting medicinal plants, Phase II was mentoring in the field, Phase III was training on making herbal products from plants and training on simple bookkeeping, Phase IV was product display. Results: Based on the results of this training showed that the knowledge of the participants related to the training material has increased which shown from the results of the posttest with average of 79,31 and product display. Conclusion: In this activity, beside gaining knowledge, participants can apply this knowledge to increase family income.
Pemberdayaan Pemuda Karang Taruna Dan Ibu-Ibu Pkk di Kelurahan Pacar Kembang, Surabaya Junairiah Junairiah; Tri Nurhariyati; Listijani Suhargo
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 3 (2020): Peran Perguruan Tinggi dan Dunia Usaha Dalam Pemberdayaan Masyarakat Untuk Menyongsong
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.366 KB) | DOI: 10.37695/pkmcsr.v3i0.1047

Abstract

Kelurahan Pacar Kembang terletak di wilayah Surabaya Timur. Permasalahan yang dihadapi oleh mitra adalah terdapat kampung yang kumuh dan belum tertata, juga pemuda karang taruna dan ibu-ibu PKK yang belum mempunyai kegiatan edukasi untuk dapat mewujudkan kampung yang bersih dan asri. Kegiatan ini bertujuan untuk mewujudkan kampung yang bersih, asri dan aman. Berdasarkan permasalahan tersebut di atas, maka permasalahan mitra yang harus segera ditangani adalah aspek lingkungan dan budidaya dengan memberikan pelatihan mural dan budidaya lidah buaya dilanjutkan dengan kegiatan praktek terhadap materi yang diberikan. Berdasarkan hasil evaluasi terhadap materi yang telah disampaikan menunjukkan bahwa rerata nilai pelatihan mural adalah 76 dan rerata nilai pelatihan budidaya lidah buaya adalah 82. Hasil praktek pembuatan mural dan pelatihan budidaya berupa foto kegiatan
Co-Authors A.A. Ketut Agung Cahyawan W AGUS SUPRIYANTO Alfiah Hayati ALIFAH NASTITI ALMANDO GERALDI ALMANDO GERALDI Almando Geraldi Ana Mariatul Khiftiyah Anjani, Ajeng Distya Annisa Azzahra, Annisa ARSY HABI PRIYATAMA BRIGITA NUR DIYAN AGUSTIANA BRUNI FLARANDA YOKU BRUNY FLARANDA YOKU CHRISTOPHER CLEMENT DANI TRI INDRIATI DANI TRI INDRIATI DELA DWI ALAWIYAH DELA DWI ALAWIYAH Diah Ayu Wulandari DWI KUSUMA WAHYUNI DWI KUSUMA WAHYUNI DWI KUSUMA WAHYUNI Dwi Kusuma Wahyuni DWI NUR RIKHMA SARI Dwi Winarni Edy Setiti Wida Utami ELVIS PERDANA PUTRA Eva Hariyanti Fatimah FATIMAH FATIMAH fatimah Fatimah FATIMAH FATIMAH Febri Dwi Irfansyah Febrionny, Princessa Nandita Firli Rahmah Primula Dewi GALUH AYU RAKASHIWI GALUH AYU RAKASHIWI GALUH AYU RAKHASHIWI Haliza Amilu Sholihah Hamidah Hamidah . Hapsari, Lukiteswari Dyah Tri Hery Purnobasuki Hery Suwito Iftitah, Daulah Intan Ayu Pratiwi, Intan Ayu Irfansyah, Febri Dwi JOHAN SETIAWAN KIRANA ATMANINDYA Lilis Sulistyorini Listijani Suhargo, Listijani Manikya Pramudya MOCH AFFANDI MOCH. AFFANDI MOCHAMMAD ILHAM MOCHAMMAD ILHAM Mochammad Affandi Muhammad Hilman Fu'adil Amin Nabilah Istighfari Zuraidassanaaz Nabilah Istighfari Zuraidassanaaz Nabilah Istighfari Zuraidassanaaz NAZIL DWI RAHAYUNINGTYAS Ni'matuzahroh Ni'matuzahroh NI'MATUZAHROH NI'MATUZAHROH Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh, Ni’matuzahroh Niken Agustin Nimatuzahroh, Nimatuzahroh Ni’matuzahroh Ni’matuzahroh NI’MATUZAHROH NI’MATUZAHROH Ni’matuzahroh, Ni’matuzahroh Ni’matuzzahroh Ni’matuzahroh Nurindah Wanti, Yulia Tri Nurul Sofi Amalia Oktavia, Gebby Agnessya Esa Purnomo Purnomo Purnomo Purnomo Putri Akustia Putri, Salsabilla Wiyana PUTUT RAKHMAD PUTUT RAKHMAD PURNAMA Ramadhani Eka Putra RC HIDAYAT SOESSILOHADI RICO RAMADHAN RICO RAMADHAN RIKA RAFFIUDIN Sa'diyah, Muhimmatus Salamun Salamun SALAMUN SALAMUN SALAMUN SALAMUN SEHANAT PRASONGSUK SEHANAT PRASONGSUK Shabrina, Afrah SILVIA KURNIA SARI SITI RIZQIYATUL MUKARROMAH SITI RIZQIYATUL MUKARROMAH Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Surahmaida, Surahmaida Syarifah Salsabila Tamalia, Aqilla Kalyca TRI NURHARIYATI Tri Anjar Tri Nurhariyati Tri Puji Lestari Sudarwati Ulinnuha, Risanda Umarudin, Umarudin Wanti, Yulia Tri Nurindah Wida Utami, Edy Setiti Yosephine Sri Wulan Manuhara Zuraidassanaaz, Nabilah Istighfari