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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biosaintifika: Journal of Biology & Biology Education Media Akuakultur
Krishanti, Ni Putu Ratna Ayu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Biological Control of Wood Destroying Organism Using Plant Extracts Collected from Mt. Merapi National Park, Indonesia Ismayati, Maya; Zulfiana, Deni; Tarmadi, Didi; Lestari, Anis Sri; Krishanti, Ni Putu Ratna Ayu; Himmi, Setiawan Khoirul; Fajar, Anugerah; Yusuf, Sulaeman
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20102

Abstract

Due to the climate change and global warming, the biodiversity database has gained the attention of the government. In line with the Indonesian Biodiversity Strategy and Action Plan (IBSAP), we have collected plants with insecticidal activity based on the local wisdom. This program aimed to protect Indonesian biodiversity from deforestation along with the loss of the number of species. This research?s goal was to evaluate termiticidal and antifungal properties from some plant extracts collected from the Turgo forest area, Mt. Merapi National park, Java. Three potential plants were evaluated. Based on specimen identification, the three plant samples were Kina (Cinchona sp.), Kamadoh (Dendrocnide stimulans (L.f.) Chew), and Keremi (Homalanthus populneus (Geiseler) Pax). The phytochemical test showed that Kina contained alkoloid, falvonoid, saponin, and tannin, whereas Kamadoh and Keremi contained saponin and tanin, respectively. Overall, all plant extracts have the termiticidal activities and able to inhibit wood- decay fungi with the inhibition percentage around 60% - 100%. Leaf extracts of Kina (Cinchona sp) and Keremi (Homalanthus populneus (Geiseler) showed the highest activity as wood-decay fungi inhibitor. The disclosure of the potential of bioinsecticides from some plants originating from Mount Merapi is very important before being lost due to deforestation and the Mount Merapi disaster. Thus, the potential bioinsecticide in these plants can increase its economic value as a substitute for synthetic insecticides that are friendly to the environment.
PRODUCTION OF CONIDIA BY ENTOMOPATHOGENIC FUNGI AND THEIR PATHOGENICITY AGAINST COPTOTERMES SP. Zulfiana, Deni; Zulfitri, Apriwi; Lestari, Anis Sri; Krishanti, Ni Putu Ratna Ayu; Meisyara, Dita
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22435

Abstract

Entomopathogenic fungi have the potential to infect most arthropods including termites which are economically important major insects pest of wood, wood products and building structures. However, the application of this fungus in the field has not shown satisfactory results yet, one of which is constrained in mass production of conidia. The purpose of this study was to evaluate 16 types of biodegradable products and waste as substrates for mass production of conidia using solid state fermentation method and two types of inoculum namely solid and liquid inoculum. Toxicity tests were carried out on subterranean termites (Coptotermes sp.) based on JIS K 1571, 2010. The parameters observed were the number and dry weight of the conidia produced, conidial viability, nutrient content of the substrate, and percentage of termite mortality. The results showed that rice, sorghum and corn were the best media for the growth of entomopathogenic fungi based on the number of conidia and dry weight of the conidia produced. Metarhizium sp. T4.B23 produced the highest number of conidia, 1.12 x 1011 conidia/100 g substrate and yielded 180.9 ± 0.623 g dry conidia/kg of rice; followed by Metarhizium sp. B2.2 grown on sorghum that resulted in 1.11 x 1010 conidia/100 g substrates and 8 ± 0.570 g /kg sorghum; and B. bassiana produced 8.3 x 109 conidia/100 g substrate and 31.24 ± 0.407 gr/ kg sorghum. Metarhizium sp. B2.2 showed the highest toxicity to termites with 100% mortality was observed within the second day of testing. Therefore the conidia of Metahizium sp. B2.2 is potential to be developed as a biopesticide using rice or sorghum substrate as a carrier.
Antimicrobial Production by an Actinomycetes Isolated from The Termite Nest Krishanti, Ni Putu Ratna Ayu; Zulfina, Deni; Wikantyoso, Bramantyo; Zulfitri, Arpiwi; Yusuf, Sulaeman
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.087 KB)

Abstract

Actinomycetes are Gram positive bacteria that have been detected in diverse ecological niches. Their member species are known to be a main source of various bioactive compounds. The discovery of Actinomycetes from diverse and unexplored resources has also been linked to increased opportunities to obtain novel bioactive compounds. Insect nest material is being investigated as a new source of novel antimicrobial producing Actinomycetes, which could be harnessed for therapeutic potential. A total of 10 Actinomycetes isolates were collected from the nest of Nasutitermes sp. in Pananjung Pangandaran Nature Reserve. These isolates were evaluated for antimicrobial activity against the challenge bacteria (Eschericia coli, Staphylococcus aureus, Bacillus subtilis, Serratia Marcescens) and fungi (Fomitopsis palustris, Fusarium oxysporum, Trichoderma viridae) by dual culture method. The result revealed that several isolate were active against fungi and bacteria. Isolate Pn-TN2 showed the highest level of antibacterial inhibition and the highest antifungal inhibition with Inhibition Rate value more than 80%. By morphological and 16S rRNA gene sequence analysis strongly suggested that the isolate Pn-TN2 belonged to Streptomyces prasinopilosus. We suggested that termite nest is a potential source of bioactive strains of cultivable Actinomycetes for future biotechnological needs.
PENGARUH BAKTERI Lactobacillus casei FREEZE DRY TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN MAS (Cyprinus carpio) Yudistira, Piero Eka; Karima, Silviyani Nurul; Rouf, Ahmad Beni; Rudi, Mad; Krishanti, Ni Putu Ratna Ayu
Media Akuakultur Vol 19, No 2 (2024): Desember 2024
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.19.2.2024.63-70

Abstract

Ikan mas (Cyprinus carpio) merupakan salah satu komoditas penting dalam budidaya perikanan air tawar. Kualitas pakan yang kurang optimal sering kali mempengaruhi pertumbuhan dan kelangsungan hidup ikan mas. Peningkatan pertumbuhan dan kelangsungan hidup ikan ini dapat didukung oleh penggunaan Lactobacillus casei probiotik freeze dry dalam pakan. Teknologi freeze dry memperpanjang penyimpanan probiotik tanpa mengurangi efektivitas dan menjaga viabilitasnya dalam pakan ikan. Penelitian ini bertujuan untuk mengevaluasi efektivitas Lactobacillus casei probiotik freeze dry terhadap pertumbuhan dan kelangsungan hidup ikan mas. Metode penelitian yang digunakan adalah eksperimental dengan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan dan tiga ulangan: PK1 (105 CFU/g), PK2 (106 CFU/g), PK3 (107 CFU/g), dan K (tanpa probiotik). Ikan mas yang digunakan berukuran 7-8 cm dengan padat tebar 10 ekor/20L air. Waktu pemeliharaan dilakukan selama 14 hari pada pemberian pakan dengan perlakuan dosis probiotik berbeda. Penambahan dosis probiotik L. casei pada pakan ikan mas menunjukkan peningkatan signifikan pada pertumbuhan dan kelangsungan hidup, dengan dosis optimal 0,1 gr/100 gr pakan (105 CFU/g), yang menghasilkan bobot mutlak (2,8 ± 0,12g), panjang mutlak (1,1 ± 0,21cm), specific growth rate (SGR) (1,7 ± 0,06%/hari), feed conversion ratio (FCR) (1,5 ± 0,06), efisiensi pemanfaatan pakan (EPP) (65,3 ± 2,27%), dan survival rate (SR) (100 ± 0%).The common carp (Cyprinus carpio) is an important commodity in freshwater aquaculture. Suboptimal feed quality often affects the growth and survival of common carp. Growth and survival of this species can be enhanced by incorporating freeze-dried probiotic bacteria into their feed. Freeze-drying technology extends the storage of probiotics without reducing their effectiveness, while maintaining viability in fish feed. This study aims to evaluate the effectiveness of freeze-dried probiotic bacteria on the growth and survival of common carp. The experimental method used was a completely randomized design (CRD) with four treatments and three replicates: PK1 (0.1 g probiotics/100 g feed, 10u CFU/g), PK2 (1 g probiotics/100 g feed, 10v CFU/g), PK3 (10 g probiotics/100 g feed, 10w CFU/g), and K (no probiotics). The common carp used were 7-8 cm in size with a stocking density of 10 fish/20 L of water. The feeding period lasted 14 days with probiotic doses applied. Adding Lactobacillus casei probiotic to the feed of carp significantly improved growth and survival, with the optimal dosage of 0.1 g/100 g of feed (105 CFU/g), resulting in absolute weight (2.8 ± 0.12g), absolute length (1.1 ± 0.21cm), specific growth rate (SGR) (1.7 ± 0.06%/day), feed conversion ratio (FCR) (1.5 ± 0.06), feed utilization efficiency (EPP) (65.3 ± 2.27%), and survival rate (SR) (100 ± 0%)
Co-Authors Ahmad Beni Rouf Deni Zulfiana Fajar, Anugerah Himmi, Setiawan Khoirul Ismayati, Maya Karima, Silviyani Nurul Lestari, Anis Sri Meisyara, Dita Rudi, Mad Tarmadi, Didi Wikantyoso, Bramantyo Yudistira, Piero Eka Yusuf, Sulaeman Zulfina, Deni Zulfitri, Apriwi Zulfitri, Arpiwi