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Journal : Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa

Uji Efektivitas Ekstrak Paku Pedang (Nephrolepis exaltata) Terhadap Pertumbuhan Jamur Candida albicans Diana Mey Merlina; Ngadiani
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 13 No 01 (2020)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.13.1.2551.33-38

Abstract

Plant swords (Nephrolepis exaltata) are ferns in the Lomariopsidaceae tribe which are easily found on the banks of rivers, cliffs, and contain flavonoids and alkaloids that can inhibit the growth of albicans candida fungi. This study aims to determine the effectiveness of Sword nail extract (Nephrolepis exaltata) on the growth of albicans candida fungi. This research is a quantitative laboratory experimental type using a completely randomized design (RAL) with 4 different treatment groups, 1 negative control (pz sterile) and 1 positive control (ketocenazole). The parameters observed were the number of colonies (CFU) and the average area of ​​the inhibition zone (mm). The data was analyzed using SPSS software using one way ANOVA analysis which was then followed by a test using DMRT. The results showed that all concentrations differed from the negative controls, while the extract concentration of 1000 ppm compared to the positive controls had no difference. Sword nail extract (Nephrolepis exaltata) has an influence on the growth of candida albicans mushrooms, namely the number of colonies of 6.78 CFU (6x106) at a concentration of 1000 ppm and inhibition zone 33.10 mm at a concentration of 1000 ppm. Keywords : Candida albicans, Sword spikes (Nephrolepis exaltata), Inhibitory Zone, Colony Count.
Pengaruh Pemberian Hormon NAA Dan BAP Pada Media MS (Murashige and Skoog) Terhadap Pertumbuhan Anggrek Vanda tricolor Secara In-Vitro Ngadiani; Try Jayanti
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 14 No 02 (2021)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.14.02.4885.89-98

Abstract

Anggrek vanda di pasar lokal maupun ekspor memiliki permintaan yang sangat tinggi hal ini tidak sebanding dengan perbanyakan vegetative anggrek vanda yang terbatas. Perbanyakan anggrek vanda dengan metode kultur jaringan merupakan solusi dalam perbanyakan bibit anggrek vanda dan dengan kultur jaringan didapatkan bibit anggrek yang berkualitas tinggi. Penelitian ini bertujuan agar mengetahui perlakuan zat pengatur tumbuh NAA dan BAP juga mengetahui konsentrasi optimum penambahan NAA dan BAP terhadap media tumbuh anggrek vanda. Penelitian ini termasuk penelitian eksperimental dimana peneliti menggunakan metode rancangan acak lengkap (RAL) dengan 4 perlakuan yang berbeda yakni MS (0ppm NAA + 0ppm BAP), MS (2ppm NAA + 2ppm BAP), MS (4ppm NAA + 4ppm BAP), MS (6ppm NAA + 6ppm BAP) diulang sebanyak 3 kali pada setiap perlakuan dan dianalisis menggunakan (ANOVA) dengan taraf signifikan (p<0.05) kemudian dilanjutkan menggunakan uji Duncan Multiple Range Test. Penelitian ini menggunakan parameter uji yakni jumlah akar, jumlah daun, berat masaa planlet dan tinggi tanaman. Hasil dari penelitian ini terdapat perbedaan signifikan ddengan pemberian beberapa perlakuan zat pengatur tumbuh NAA dan BAP pada medi MS. Perlakuan B (MS + 2ppm NAA + 2ppm BAP) merupakan perlakuan terbaik dalam mendapatkan hasil optimum guna meningkatkan jumlah akar dan berat massa planlet anggrek vanda. Hasil pada penelitian mampu menjadi referensi bagi pembudidaya kultur jaringan tanaman anggrek vanda. Pembudidaya disarankan menggunakan zat pengatur tumbuh dengan konsentrasi 2ppm NAA dan 2ppm BAP untuk meningkatkan pertambahan jumlah akar dan berat massa planlet anggrek vanda. Kata Kunci : Anggrek Vanda, BAP, Media MS, NAA dan Kultur Jaringan.
Respon Pertumbuhan Tanaman Anggrek Bulan (Phalaenopsis amabilis) Terhadap Perlakuan Media Tanam dan POC Kulit Bawang Merah (Allium cepa) Firdaus El Salama, Shaila; Ngadiani; Andriani, Vivin
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 17 No 01 (2024)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.17.01.9173.132-139

Abstract

Moon orchid (Phalaenopsis amabilis) is one of the most fascinating orchid genus. The variety of colors, shapes and textures, as well as the aroma make moon orchids one of the most beautiful flowers. Ferns contain elements of Nitrogen (N), Carbon (C), Hydrogen (H), and silica. Coconut shells contain carbohydrates (K), phosphorus (P), sodium (Na) and magnesium (Mn). Shallots (Allium cepa) contain the elements Potassium (K), Calcium (Ca), Nitrogen (N), Phosphorus (P), Iron (Fe), Manganese (Mg), Magnesium (Mn), and Zinc (Zn). Meanwhile, non-nutritive chemical compounds include auxin and gibberellin hormones. This study used the complete randomized design (CRD) method, which consisted of 18 treatments and 3 replications. The growth parameters observed were number of leaves, leaf area, root length, plant weight, and survival rate. Based on the results of this study, it is concluded that the provision of planting media and shallot skin POC treatment affects the growth of moon orchids. The results of Duncan's test showed the M3P4 treatment combination (chopped fern + coconut shell) and shallot skin POC (30%) produced the best growth in the parameter of root length of 5.16 cm and plant weight of 2.82 grams. However, it has no effect on the number of leaves and leaf area. Keywords: Moon orchid; Growing media; POC; Chopped fern, coconut shell; Shallot skins.