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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Marine Research in Indonesia Makara Journal of Science
Nuchsin, Ruyitno
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Veterinary

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HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS Darmayati, Yeti; Harayama, Shigeaki; Yamazoe, Atsushi; Hatmanti, Ariani; Sulistiani, -; Nuchsin, Ruyitno; Kunarso, Djoko Hadi
Marine Research in Indonesia Vol 33 No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9627.525 KB) | DOI: 10.14203/mri.v33i1.506

Abstract

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It?s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.
SCREENING BAKTERI PENGHAMBAT UNTUK BAKTERI PENYEBAB PENYAKIT PADA BUDIDAYA IKAN KERAPU DARI PERAIRAN BANTEN DAN LAMPUNG Hatmanti, Ariani; Nuchsin, Ruyitno; Dewi, Julinasari
Makara Journal of Science Vol. 13, No. 1
Publisher : UI Scholars Hub

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Screening for Inhibitor Bakteri for Pathogenic Bacteria in Groupers Culture in Banten and Lampung Waters. A research about screening of inhibitor bacteria for pathogenic bacteria in grouper culture was done during 2005 in Marine Microbiology Laboratory, Research Center for Oceanography LIPI. Inhibition test was done using paper disc method for three isolates of pathogenic bacteria in his research, they were: Vibrio harveyii, 9.2/Luka/TSA and 8.2/Luka/TSA. The result shows that from 39 isolates of inhibitor bacteria from Banten and 27 isolates of inhibitor bacteria from Lampung only two isolates showed inhibition effect to the growth of three isolates of pathogenic bacteria. found inhibit the three strains of pathogenic bacteria mentioned above. The two isolates of inhibitor bacteria were 9L/AL-4/KNG/BLC/BOKN/BJN and 5L/AL-4/KNG/BJN, which were isolated from Bojonegara, Banten.
DISTRIBUSI VERTIKAL BAKTERI DAN KAITANNYA DENGAN KONSENTRASI KLOROFIL-a DI PERAIRAN KALIMANTAN TIMUR Nuchsin, Ruyitno
Makara Journal of Science Vol. 11, No. 1
Publisher : UI Scholars Hub

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Vertical distribution of bacteria population in relation to chlorophyll-a in East Kalimantan waters. Study on distribution of bacteria population and its relation to concentration of chlorophyll-a has been conducted in August – September 1999 in East Kalimantan waters. The purpose of the study was to observe the correlation between population of bacteria and concentration of chlorophyll-a in water column. Acridine Orange Epifluorescence Microscopy method was used to analyze bacteria population, while fluorometric method was used to determine chlorophyll-a concentration. The result of the study showed that bacteria population was positively correlated to chlorophyll-a concentration, area with high bacteria population has high concentration of chlorophyll-a. The high bacteria population was found in the water columnh of the 25 m deep,as well as for chlorophyll-a concentration, ranged between (4 and 90) x 106 cell per ml and (0.2 and 1.14) mg per m3 respectively. It was concluded that vertical distribution of bacteria population was closely correlated to the concentration of chlorophyll-a
Optimization Bacterial Density and Fertilizer Dosage for Bioremediation of Oil Contaminated Sandy Beach: A Case of Cilacap, Indonesia Darmayati, Yeti; Sanusi, Harpasis S.; Prartono, Tri; Santosa, Dwi Andreas; Nuchsin, Ruyitno
BIOTROPIA Vol. 24 No. 3 (2017): BIOTROPIA Vol. 24 No. 3 December 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.3.611

Abstract

Bioremediation, involving biostimulation and/or bioaugmentation, is a promising method to overcome oil spills in Cilacap coastal waters. Cilacap coastal area has high risk on oil pollution. This study investigated the stimulatory effect of nitrogen concentration, bacterial density and the composition of bacterial culture in enhancing oil degradation in this area. The applications of 4 different concentrations of Slow Release Fertilizer (SRF) and 2 different densities of bacterial cells in the form of single (RCO/B/08_008) and mixed culture were employed in microcosm experiments for 28 days. The efficacy of combining bacterial culture and fertilizer application in various concentrations was also tested. Oil degradation, bacterial growth and environmental parameters were monitored periodically during the experiments. The results showed that oil degradation rate was more influenced by nutrient concentration (biostimulation) than bacterial number or culture composition (bioaugmentation) added. The efficacy of biostimulation in degrading oil was better than that of bioaugmentation. Biostimulation increased oil degradation up to 6.4 times higher than the control. The optimum of fertilizer concentration added was 7.5 mg N/g (C:N ratio of 1,000:75), which increased depletion rate both in biostimulation-only and the combination of biostimulation with bioaugmentation up to 6.4 and 7.5 times higher than the control, respectively. It is suggested that bioremediation of oil-contaminated sandy beach in Cilacap would be optimal by employing a combination of Slow Release Fertilizer at concentration having C/N ratio = 1,000 : 75 and RCO/B/08_008 culture at density of 0.5 x 108 cells/mL (100% homology with Alcanivorax sp. TE-9).
Co-Authors Ariani Hatmanti Dewi, Julinasari Djoko Hadi Kunarso DWI ANDREAS SANTOSA Harayama, Shigeaki Harpasis S. Sanusi Sulistiani, - Tri Prartono Yamazoe, Atsushi Yeti Darmayati