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All Journal OLDI (Oseanologi dan Limnologi di Indonesia) Bioma : Jurnal Biologi Indonesia Marine Research in Indonesia Makara Journal of Science

Ariani Hatmanti

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Author-ID : 278604

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology

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Articles

Diversity of Culturable Actinomycetes from Deepsea Floor of Makassar Strait, Indonesia Hatmanti, Ariani; Lisdiyanti, Puspita; Widada, Jaka; Wahyuono, Subagus
Oseanologi dan Limnologi di Indonesia Vol 3, No 2 (2018)
Publisher : Oseanologi dan Limnologi di Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oldi.v3i2.205

With regard to collaboration research called Widya Nusantara Exploration (EWIN) in May-June 2013 and November 2014, a study on isolation of actinomycetes from sediments of Makassar Strait have been conducted. Actinomycetes is one of microbe which has an excellent track record in producing antimikrob and other active substances. But due to terrestrial actinomycetes has been widely explored, then recently researchers began focusing on wide variety of extreme environments, such as marine environment, to screening aktinomisetes in producing new secondary metabolites. A total of 36 strains of actinomycetes were isolated from 10 samples obtained from deepsea floor in Makassar Strait, Indonesia, Direct Dillution Method were best used to isolate the actinomycetes compare to Sodium Dodecyl Sulfida â Yeast Extract Method (SDS-YE Method) and Rehidration Centrifugation Method (RC Method). NBRC-802 media and Actinomycetes Isolation Agar(AIA)(Himedia)media were used as the isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. Actinomycetes isolated from deepsea floor of Makassar Strait have been dominated by Micromonospora (58%), Verrucosispora (14%)Streptomyces (8%) and Luteipulveratus (5%), however genus Nocardiopsis, Micrococcus, Gordonia, Kytococcus, and Arthrobacter were not dominant (3%). Station 25 in 1.547 m depth was the most abundant of actinomycetes, 18 strains and dominated by the genus Micromonospora which is isolated using Direct Dillution Method and both NBRC 802 or AIA media.

HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS Darmayati, Yeti; Harayama, Shigeaki; Yamazoe, Atsushi; Hatmanti, Ariani; Sulistiani, -; Nuchsin, Ruyitno; Kunarso, Djoko Hadi
Marine Research in Indonesia Vol 33, No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.Itâs sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.

HYDROCARBONOCLASTIC BACTERIA FROM JAKARTA BAY AND SERIBU ISLANDS Darmayati, Yeti; Harayama, Shigeaki; Yamazoe, Atsushi; Hatmanti, Ariani; Sulistiani, -; Nuchsin, Ruyitno; Kunarso, Djoko Hadi
Marine Research in Indonesia Vol 33 No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Jakarta Bay has been known as one of the most polluted marine environment in Indonesia, with no exception by oil. Seribu Islands waters, located in the north of Jakarta Bay may have been impacted by this polluted condition.It?s sometimes also hit by oil spillage from pipe leakage. The purpose of this study is to isolate and identify hydrocarbonoclastic bacteria (oil and Polyaromatic Hydrocarbon degrading bacteria) from Jakarta Bay and Seribu Island waters. The bacteria were isolated from water and sediment/sand. Isolation was prepared by enriched samples in SWP medium with Arabian Light Crude Oil (ALCO). Screening for PAH degrading bacteria has been completed by using sublimation plate method in ONR7a medium and screening for oil degrading bacteria were conducted by using oil plated method with the same medium. Bacteria identifications were done based on l6sRNA gene. The results were analyzed using BLAST and showed that 131 potential hydrocarbonoclastic bacteria have been isolated from Jakarta Bay and Seribu Island waters. Most of them were oil degrading bacteria (41.98%) and the rest were PAH degrading bacteria. Oil pollution level may impact the number of strain of hydrocarbonoclastic bacteria isolated. Among the hydrocarbonoclastic bacteria isolated from Jakarta Bay and Seribu Islands, Alcanivorax, Marinobacter, Achromobacter and Bacillus were common hydrocarbonoclastic genera in Jakarta Bay and its surrounding waters. Alcanivorax spp. is important oil and PAH-degrader found not only in temperate waters, but in tropical waters as well.

SCREENING BAKTERI PENGHAMBAT UNTUK BAKTERI PENYEBAB PENYAKIT PADA BUDIDAYA IKAN KERAPU DARI PERAIRAN BANTEN DAN LAMPUNG Hatmanti, Ariani; Nuchsin, Ruyitno; Dewi, Julinasari
Makara Journal of Science Vol. 13, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Screening for Inhibitor Bakteri for Pathogenic Bacteria in Groupers Culture in Banten and Lampung Waters. A research about screening of inhibitor bacteria for pathogenic bacteria in grouper culture was done during 2005 in Marine Microbiology Laboratory, Research Center for Oceanography LIPI. Inhibition test was done using paper disc method for three isolates of pathogenic bacteria in his research, they were: Vibrio harveyii, 9.2/Luka/TSA and 8.2/Luka/TSA. The result shows that from 39 isolates of inhibitor bacteria from Banten and 27 isolates of inhibitor bacteria from Lampung only two isolates showed inhibition effect to the growth of three isolates of pathogenic bacteria. found inhibit the three strains of pathogenic bacteria mentioned above. The two isolates of inhibitor bacteria were 9L/AL-4/KNG/BLC/BOKN/BJN and 5L/AL-4/KNG/BJN, which were isolated from Bojonegara, Banten.

ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL SENYAWA ANTIBAKTERI YANG BERASOSIASI DENGAN KARANG BATU DARI PERAIRAN BITUNG DAN SPONS DARI SELAT MAKASSAR SUNNY, FRANSISCA; KURNIATI, TRI HANDAYANI; HATMANTI, ARIANI
Bioma Vol. 11 No. 1 (2015): Bioma
Publisher : LPPM Universitas Negeri Jakarta

ABSTRACT Recently the needs of antibacterial compounds is increasing. This is due to the bacterial resistence to common antibacterial compounds. coral and sponge-ssociated bacteria are potential producer of antibacterial compounds. This research was aim to obtain coral and sponge-associated bacteria that could produce antibacterial compound. coral associated-bacteria was isolated from Bitung and was isolated in Marine Agar by pour plate method. The antibacterial compounds were obtained by extraction using ethyl acetate and acetone. The antibacterial assay was performed by agar diffusion method using paper discs and was performed by testing with Staphylococcus aureus, Bacillus subtilis, Vibrio cholerae biotipe El Tor, and Escherichia coli. Total 37 isolate was isolated from corals and 25 isolate from sponge obtained from Selat Makassar. Based on the assay, only bacteria from sponge that showed antibacterial activity. Two sponge-associated bacteria, S.5-8 and S.2-1 NRBC were found to inhibit S. aureus. From those isolates, isolate S.5-8 produced bigger clear zone (2,6 mm) than S.2-1 NRBC (1,5mm). S.5-8 could hydrolize gelatine whereas S.2-1 NRBC showed positive result on oxidase test and was able to fement xilose and arabinose to produce acid. Key words: antibacterial activity, association, characterization, coral, isolation, sponge

Co-Authors Dewi, Julinasari Djoko Hadi Kunarso Harayama, Shigeaki Harayama, Shigeaki JAKA WIDADA Nuchsin, Ruyitno Nuchsin, Ruyitno PUSPITA LISDIYANTI Subagus Wahyuono Sulistiani, - Sulistiani, - SUNNY, FRANSISCA Tri Handayani Kurniati Yamazoe, Atsushi Yamazoe, Atsushi Yeti Darmayati

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