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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
Bintara, S.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

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Association of IGFBP-3 gene polymorphism g. 3.930 G>A with birth size and birth weight in crossbred beef cattle Hartatik, T.; Priyadi, D. A.; Panjono, P.; Bintara, S.; Ismaya, I.; Budisatria, I. G. S.; Widyobroto, B. P.; Agus, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.356-363

Abstract

Crossing between cattle breeds is the one way to improve the genetic quality of cattle. Controlling crosses using genetic markers will speed up the process. The IGFBP-3 gene is a potential marker of selection due to its role in regulating growth and metabolism in cattle. The aim of this study was to determine the effect of genotype on birth size and birth weight using IBFBP-3 gene marker. A total of 32 cattle consisted of 10 Brahman Cross cattle, 14 cattle results of Brahman Cross cow crosses with Belgian Blue bull, and 8 cattle results of Brahman Cross cow crosses with Wagyu bull. Sequences of 563 bp, including the part intron 2, exon 3, and the part intron 3. Genotyping was done by sequencing method and PCR-RFLP method with the restriction enzyme PvuII. An SNP was identified at location g 3,930 (G>A). Two genotypes were observed, namely GA and GG. The genotype did not affect the performance of birth (body weight, body length, height at withers, heart girth) of the research sample. Birth traits are influenced by the cattle breed, it can be seen that Brahman Cross cattle have a body length that is bigger than the other crossbred.
Analysis in making decision of farmer to select bull frozen semen in Indonesia Agustine, R.; Bintara, S.; Andarwati, S.; Muzayyanah, M. A. U.; Widi, T. S. M.; Putra, A. R. S.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.323-332

Abstract

The research objectives was to analyze the farmer’s decision in selecting breed of bull frozen semen for Artificial Insemination (AI) and determine the factors influencing the decision. There were five bull frozen semen options which simulated in this research, such as Simmental, Limousine, Ongole Grade (Peranakan Ongole / PO), Brahman, and other option. ”Other” option was used if the farmer did not recognize what breed of frozen semen was inseminated to their cow. Farmers must choose only one option of bull frozen semen that suitable for their cow. This study was conducted by collecting data using questionaire from 400 beef cattle’s farmers in Central Java Province and Yogyakarta Province. Respondents were selected by multistage random sampling technique. Descriptive statistical analysis and multinomial logistic regression analysis was used in this study The results revealed that 42% of farmers were more interested in inseminating their cows with bull semen from Simmental, and 30.75% of farmers were more interested in Limousin. Other variables such as family member, AI’s cost, land ownership, cow breed which lastly being inseminated, geographical factor, and farmer’s knowledge about the inseminatorswere variables that determine farmer’s decision process.
Nutrient Digestibility, N Balance, Performance, and Blood Parameters of Kacang Goats Differing in GDF9 Genotype Fed Different Sources of Dietary Fiber Irawan, A.; Hartatik , T.; Bintara, S.; Astuti, A.; Kustantinah
Tropical Animal Science Journal Vol. 47 No. 1 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.1.33

Abstract

This study aims to determine the impact of different GDF9 genotypes on feed intake, nutrient digestibility, and nitrogen balance in Kacang goats by examining various metabolic processes. Twenty-nine Kacang goats were genotyped using PCR-RFLP DNA at position g.3855A/C of the GDF9 gene in exon 2. A 2 × 2 factorial design with two factors, namely, diet type (diets 1 and 2) and genotype (homozygote AA and heterozygote AC), was adopted. Diet 1 group comprised 11 goats with the AA genotype and 4 goats with the AC genotype, and the diet 2 group consisted of 9 goats with the AA genotype and 5 goats with the AC genotype. Both diets had the same protein content (iso-protein) and consisted of free-choice Napier grass (Pennisetum purpureum). Diet 1 had an additional 400 g of concentrate containing 11.25% crude protein (CP) and 55.86% total digestible nutrients (TDN). Diet 2 was a total mixed ration (TMR) containing 12.46% crude protein and 67.92% TDN. Results demonstrated a significant interaction (p<0.05) effect between diet and genotype on crude protein (CP) digestibility, neutral detergent fiber (NDF) digestibility, fecal nitrogen (N feces), urinary nitrogen (N urine), nitrogen retention (%), and blood urea nitrogen (BUN). However, no significant interaction effect was observed on the consumption of dry matter (DM), CP, crude fiber (CF), nitrogen free extract (NFE), and NDF and the digestibility of DM, CF, and NFE. TMR resulted in a 9% higher consumption of NDF, higher nutrient digestibility, improved nitrogen balance, average daily gain (ADG), and elevated glucose levels compared with diet 1. Kacang goats with the AC genotype exhibited better CP digestibility and increased nitrogen intake than those with the AA genotype. In conclusion, the GDF9 genotype influences the nutrient digestibility in Kacang goats, and those with the AC genotype utilize feed nutrients more efficiently than those with the AA genotype. The AC genotype resulting from the GDF9 gene mutation at position g.3855A>C can be used for genomic marker selection of high-quality Kacang goats in nutrient digestibility.
Co-Authors A. Agus A. Astuti, A. Agustine, R. B. P. Widyobroto Budisatria, I. G. S. Hartatik , T. I. Ismaya Irawan, A. Kustantinah Muzayyanah, M. A. U. Priyadi, D. A. Putra, A. R. S. S. Andarwati T. Hartatik Widi, T. S. M.