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All Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)
Arjuna, Andi
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Uji Pendahuluan Anti-biofilm Esktrak Teh Hijau dan Teh Hitam Pada Streptococcus mutans melalui Metode Microtiter Plate: An initial study on anti-biofilm activity of green tea dan black tea extracts on Streptococcus mutans via mictotiter plate assay Arjuna, Andi; Pratama, Winda Setya; Sartini, Sartini; Mufidah, Mufidah
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 4 No. 1 (2018): (March 2018)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.686 KB) | DOI: 10.22487/j24428744.2018.v4.i1.9965

Abstract

Tea (Camellia sinensis L.) has an activity as an antibacterial, widely studied to plankton cells, without further researching into biofilm cell. Therefore, this research had been conducted to initially evaluate the activity of green- and black-tea extracts in inhibiting Streptococcus mutans biofilm. Green and black tea leaves were extracted using 70% methanol. Determination of MIC was subsequently performed by microdilution method. Next, the biofilm formation and inhibition were run through microtiter plate method using flexible U-bottom PVC 96 wells, which then observed using microplate reader on λ = 515 nm. As The results, MIC for green and black tea extract stood at 4 mg/mL, 6 mg/mL respectively. The biofilm inhibitory activity of black tea extract was at 8 and 10 mg/mL inhibiting 6 % and 12.5 % S. mutans. Green tea extract showed that concentration of 4 to 10 mg/mL was able to inhibit biofilm growth by 24%; 45%; 48% and 53%. Thus, through microtiter plate assay, it could be concluded that tea extract has potent antibiofilm to S. mutans, where green tea extract has better activity than black tea extract.
Modifikasi Serat Limbah Kubis Menjadi Nanokristalin Selulosa Melalui Metode Hidrolisis Asam: Waste Cabbage Fibers Modification Into Nano-Crystalline Cellulose Via Acid Hydrolysis Method Arjuna, Andi; Natsir, Selva; Khumaerah, Andi Amelia; Yulianty, Risfah
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 4 No. 2 (2018): (October 2018)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.591 KB) | DOI: 10.22487/j24428744.2018.v4.i2.11093

Abstract

As one of vegetable plants in South Sulawesi, cabbage (Brassica oleracea L.) crops has generated cellulose fibers biomass which is potentially modified into nano-crystalline cellulose, a valuable material in the pharmaceutical formula. Therefore, this study aims to manipulate the natural cellulose fibers of cabbage biomass through acid hydrolysis method within product preliminary evaluation through FT-IR and XRD. The fibers were modified through the bleaching process produce micro crystalline cellulose, which was then hydrolyzed with 65% sulfuric acid to obtain nanocrystalline cellulose. The products have yellow pale to brown colour, with a yield of 10.06% and 31.16% respectively. Based on FT-IR spectra, both products inherit cellulose characteristics, C-O (1232.16 cm-1); C = O (1743.65 cm-1); -OH (1625.99 cm-1); C-H (2920.23 cm-1); O-H (3414 cm-1). The increasing trend of crystallinity index during the process was also observed in XRD diffractogram. It is identifiable from 7.41% for natural fiber, 69.68% for crystalline microcrystalline, and 78.01% for nano crystalline cellulose. Through Match®, the estimated crystalline product size reaches 58.91 nm.
Co-Authors Khumaerah, Andi Amelia Mufidah Mufidah Natsir, Selva Pratama, Winda Setya Risfah Yulianty Sartini Sartini