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All Journal Global Medical and Health Communication Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Penelitian Pendidikan IPA (JPPIPA) Folia Medica Indonesiana
Margiana, Ria
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Effect of Umbilical Cord-derived Mesenchymal Stem Cell Secretome on Corpus Cavernosum Elastography Results in Patients with Severe Erectile Dysfunction Non-responsive to Sildenafil Pratama, Andi Ahmad Thoriq; Rahardjo, Paulus; Atmaja, M Hidayat Surya; Supardi, Petrus; Margiana, Ria
Global Medical & Health Communication (GMHC) Vol 13, No 1 (2025)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v13i1.14144

Abstract

Erectile dysfunction is one of the most common male sexual dysfunctions, defined as the inability to get or maintain an erection, and its prevalence is increasing over time. The current treatment, using a phosphodiesterase-5 inhibitor like sildenafil, only offers a temporary solution. Secretome, a bioactive substance derived from mesenchymal stem cells from the umbilical cord, has shown remarkable regenerative capabilities. This study aims to explore the potential of administering umbilical cord-derived mesenchymal stem cell secretome to patients with severe erectile dysfunction and evaluate its impact on the stiffness of the corpus cavernosum using elastography in the flaccid phase. This study employed a pre-experimental design with a pre-post test. It involved seven patients who came to Dr. Soetomo Hospital with severe erectile dysfunction that was unresponsive to sildenafil. Elastography evaluation, including strain and shear wave elastography, was performed before and one month after secretome injection, which was carried out from March to April 2024. Data were analyzed using the paired sample t-test when the data distribution was normal and the Wilcoxon test when the data distribution was not normal. There were statistically significant changes (p<0.05) in all patients who underwent strain and shear wave elastography of the corpus cavernosum before and one month after secretome administration, indicating an improvement in stiffness. In conclusion, intracavernosal administration of umbilical cord-derived mesenchymal stem cell secretome has demonstrated promising results in treating severe erectile dysfunction non-responsive to sildenafil therapy. These results include improved penile tissue in the corpus cavernosum, as evidenced by decreased stiffness on elastography.
Evaluating the effectiveness of Thiel embalming solution for preserving cadavers in anatomy and surgical education Indrawan, I Made Rayo Putra; Margiana, Ria
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 9 No 01 (2025): Qanun Medika Vol 09 No 01 January 2025
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v9i01.21946

Abstract

Cadavers have played a crucial role in anatomy teaching, owing to their resemblance to the anatomical structures found in living individuals. To optimize the utilization of cadavers, embalming procedures that involve the application of chemical fixative agents are needed. The "soft embalming" approach, which involves various salts as fixating agents, was first developed by Walter Thiel. Although the Thiel solution has demonstrated efficacy in enhancing tissue elasticity, consistency, and color, a study is needed to find the most suitable for embalming with this solution to maximize its utility in the context of anatomy and surgical education. The study results show that the Thiel solution produces the best outcomes for its flexibility, uniformity, and color, which is optimal for anatomical and surgical teaching. The efficacy of Thiel's solution for preserving cadavers for educational intentions is noteworthy for the study of anatomy and surgical procedures. The advantageous characteristics of Thiel embalming include flexibility, consistency, and natural coloring. Nevertheless, it is crucial to consider the restrictions associated with preserving the brain, spinal cord, eye, and musculoskeletal tissues. In conclusion, the utilization of Thiel solution has proven to be an efficient embalming agent for preserving cadavers in the fields of anatomy and surgery education, besides brain, spinal cord, eye, and musculoskeletal systems, should be reconsidered.
The Success Rate of Cryopreservation and Thawing of Embryos at Day 3 and Day 5 Following Intracytoplasmic Sperm Injection (ICSI) Margiana, Ria; Wen, Maitra Djiang
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.9327

Abstract

Cryopreservation, the technique of freezing and thawing embryos, is essential in in vitro fertilization (IVF) treatments to ensure the long-term preservation of embryos for future use. This innovative study evaluates the effectiveness of these procedures conducted at a private hospital in Tangerang, Indonesia, over a period of seven years (2016-2023), involving 18,000 couples. The design research was conducted retrospectively. The study examined embryos that underwent cryopreservation and subsequent thawing, with data collected on the rates of cell division observed on Day 3 and the rates of blastocyst formation on Day 5 after thawing. The study investigated the rates of cellular division on Day 3 and the development of blastocysts on Day 5 following the thawing process. The findings demonstrated a remarkable cleavage rate of 95.24% and a blastocyst rate of 97.22%. The remarkable results underscore the efficacy of the advanced freezing and thawing techniques employed. This discovery is highly significant since there is an urgent requirement to improve the success rates of in vitro fertilization (IVF) for couples facing infertility. It offers a fresh sense of hope and increased opportunities for establishing successful pregnancies with the improvement of cryopreservation techniques.
Centella asiatica Nanoparticles as Potential Acetylcholinesterase Inhibitor for Cognitive Decline Therapy using Ellman's Method: An in Vitro Study Nathania, Nathania; Kusumaningrum, Selvina Cindy; I'tishom, Reny; Kumalasari, Feranita; Margiana, Ria
Folia Medica Indonesiana Vol. 60, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: 1. This research contributes novel data to current studies by combining the potential of Centella asiatica extract with PEG-400 to develop a therapeutic agent aimed at improving cognitive function through an effective drug delivery system capable of penetrating the blood-brain barrier. 2. The findings of this study revealed that the combination of Centella asiatica extract and PEG-400 at an adequate ratio exhibits great potential as an acetylcholinesterase inhibitor. Abstract Cognitive impairment, caused by neurocognitive changes and neuroinflammation, affects 65.6 million elderly people worldwide and can interfere with their quality of life. Centella asiatica is recognized for its neuroprotective potential due to its active compounds. This study aimed to investigate the acetylcholinesterase inhibitory properties of Centella asiatica as potential therapeutic agents for cognitive decline. Polyethylene glycol 400 (PEG-400) was used to achieve an effective drug delivery system of Centella asiatica extract, facilitating the inhibition of the apoptosis signaling pathway and allowing neuroprotective agents to cross the blood-brain barrier (BBB). This research involved several testing stages, including gas chromatography-mass spectrometry (GC-MS) to identify active compounds (e.g., tryptamine, γ-sitosterol, and β-sitosterol) that contribute to cognitive function improvement. Particle size analysis (PSA) tests were conducted on three formulations of the extract and PEG-400, with ratios of 1:100, 100:1, and 1:1, to determine the optimal formulation for subsequent testing. Scanning electron microscopy (SEM) was utilized to observe the morphology and surface structure of the samples, while Ellman's method was employed to test the ability of acetylcholine (ACh) in improving cognitive abilities. The results subsequently underwent descriptive analysis, particle distribution analysis, analysis of variance (ANOVA), nonparametric tests, image analysis, regression tests, multivariate analysis, and correlation tests. The synthesis demonstrated that the 1:100 formulation produced ideal-sized nanoparticles (5–7 nm), optimal for penetrating the BBB. The PSA and SEM analyses supported this finding by demonstrating homogeneous particle morphology and consistent chemical composition. The in vitro Ellman's assay revealed a high inhibitory rate of 97.63% for the 100:1 formulation. The 1:1 and 1:100 formulations demonstrated a very high effectiveness as acetylcholinesterase inhibitors. The combination of PEG-400 and Centella asiatica extract has great potential as an innovative pharmacological therapy for cognitive decline. However, further research is required to ensure the right dosage and development of the research findings.
ANALYSIS OF PENIS SIZE DIFFERENCES IN PATIENTS WITH SEVERE ERECTILE DYSFUNCTION NON-RESPONSIVE PDE5i WITH MESENCHYMAL STEM CELL SECRETOME FROM UMBILICAL CORD Wibowo, Kristophorus Jonathan; I’tishom, Reny; Soebadi, Mohammad Ayodhia; Atmaja, Muhammad Hidayat Surya; Supardi, Supardi; Margiana, Ria; Amalia, Fatkhurrohmah Leo
Folia Medica Indonesiana Vol. 61, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

The ineffectiveness of PDE5i[-10pc]AU: Please provide the affiliation of all authors. therapy is indicated by the patient’s unresponsiveness to oral medication, evidenced by insufficient erection after four attempts with the dosage recommended by the manufacturer. Consequently, the application of regenerative therapy may be seen as an effort to stimulate a response or improvement in erectile function, especially in individuals with erectile dysfunction who exhibit no significant progress after utilizing PDE5 inhibitors. Regenerative therapy seeks to repair damaged erectile tissue, with the objective of enhancing erectile function and rehabilitating tissue impacted by existing comorbidities. The secretome denotes the aggregate of chemical compounds and biological factors secreted by cells into the extracellular environment. These secretory factors are essential for various biological processes, including maintaining homeostasis, promoting tissue growth and repair, regulating immune responses, controlling inflammation, stimulating angiogenesis, inducing apoptosis, degrading proteins, facilitating cell adhesion, and organizing the extracellular matrix (ECM). We calculated the sample size using a normal significance level with α = 0.05 and a standard power corresponding to β= 0.20. Metode for this research used a pre–post test design and was conducted from April to May 2024 at the Andrology Clinic of Dr. Soetomo General Academic Hospital, Surabaya, involving a sample of 7 patients with severe ED who were non-responders to PDE5i and had type 2 diabetes mellitus. SPL, FPL, and penile circumference were evaluated before and one month after intracavernosal injection of UC-MSC secretome. This study revealed substantial variations in SPL, FPL, and penile circumference among erectile dysfunction patients who were non-responders to PDE5i, both prior to and following the infusion of mesenchymal stem cell-derived secretome from the umbilical cord. SPL rose by 0.715 cm (p-value: 0.047), FPL was increased by 0.643 cm (p-value: 0.022), and penile circumference was enlarged by 0.50 cm (p-value: 0.014). These enhancements were noted on day 30 post-data collection, It is concluded that intracavernosal secretome UC-MSC therapy for severe PDE5i-nonresponsive ED demonstrates improvement in penile blood flow; however, further studies with longer evaluation periods are still needed to obtain more robust results.
Co-Authors Atmaja, M Hidayat Surya Atmaja, Muhammad Hidayat Surya Fatkhurrohmah Leo Amalia I'tishom, Reny Indrawan, I Made Rayo Putra I’tishom, Reny Kumalasari, Feranita Kusumaningrum, Selvina Cindy Mohammad Ayodhia Soebadi Nathania Nathania, Nathania Paulus Rahardjo, Paulus Pratama, Andi Ahmad Thoriq Supardi Supardi Supardi, Petrus Wen, Maitra Djiang Wibowo, Kristophorus Jonathan