Pratiwi, Donna
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EFEK ANTI BAKTERI EKSTRAK KULIT JERUK NIPIS (CITRUS AURANTIFOLIA) TERHADAP SALMONELLA TYPHI SECARA IN VITRO Pratiwi, Donna; Suswati, Irma; Abdullah, Mariyam
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 9, No 2 (2013): Desember 2013
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.22219/sm.v9i2.4139

Abstract

Efek Antimikroba Ekstrak Kulit Jeruk Nipis (Citrus aurantifolia) terhadap Salmonella typhi secara In Vitro. Demam tifoid merupakan infeksi akut saluran pencernaan yang disebabkan oleh yaitu Salmonella typhi yang berada pada urutan kedua di antara penyakit usus setelah gastroenteritis. Peningkatan resistensi Salmonella terhadap antimikroba salah satunya kloramfenikol sudah mulai tinggi, sehingga menimbulkan masalah dalam terapi. Kulit jeruk nipis diduga memiliki efek antimikroba karena mengandung minyak atsiri, flavonoid, tanin, saponin, fenol dan alkaloid. Tujuan penelitian adalah untuk membuktikan efek ekstrak kulit jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan bakteri Salmonella typhi. Penelitian ini menggunakan true eksperimental post test only control. Konsentrasi ekstrak kulit jeruk nipis yang digunakan adalah 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,78%, 0,39%, 0%. Analisa data menggunakan one way ANOVA, Korelasi, Regresi, Kadar Hambat Minimum, Kadar Bunuh Minimum. Signifikan 0,000 (P<0,05) pada uji one way ANOVA menunjukkan adanya perbedaan bermakna antar perlakuan pada jumlah koloni. Semakin tinggi ekstrak kulit jeruk nipis, maka semakin sedikit koloni bakteri Salmonella typhi (r= -0,586). Pemberian konsentrasi ekstrak kulit jeruk nipis berpengaruh terhadap penurunan jumlah koloni bakteri Salmonella typhi (R2=0,34). Hasil penelitian KHM didapatkan pada konsentrasi 12,5%, dan KBM pada konsentrasi 6,25%. Ekstrak kulit jeruk nipis memiliki efek antimikroba terhadap Salmonella typhi.
PREVALENSI HASIL POSITIF SIFILIS DENGAN METODE PEMERIKSAAN ELISA DAN CLIA DI UDD PMI KOTA DENPASAR PERIODE MEI -DESEMBER 2021 Pratiwi, Donna
E-Jurnal Medika Udayana Vol 11 No 9 (2022): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2022.V11.i9.P08

Abstract

Introduction. One of the sexually transmitted diseases is syphilis which is caused by the bacterium Treponema pallidum. Patients with syphilis can experience severe systemic symptoms and can cause disability. However, in latent syphilis the initial symptoms are minimal or even asymptomatic, so that undetected syphilis sufferers can still escape and can carry out blood donation activities. To meet the demand and need for blood in Denpasar City, the Indonesian Red Cross Blood Donor Unit Denpasar City conducted blood donation activities. However, due to the ongoing Covid 19 world pandemic, including Indonesia, the activities of mobile blood donor units are limited. Providing safe blood is the duty of the blood donor unit. Screening test is a way to detect the presence of Transmitted Infection Through Blood Transfusion (IMLTD). The techniques used are Enzyme-linked Immunoserbent Assay (ELISA) and Chemiluminescence Immunoassay (CLIA). Methods. This research is a retrospective descriptive type by taking secondary data from the screening laboratory of the Denpasar City PMI blood donor unit for the period May-December 2021. Results. There were 21 blood samples with reactive syphilis results from May to August which were performed using the ELISA method with a total of 831 donor bags. A total of 28 blood samples from 1,338 bags that were donated showed reactive results from September to December using the CLIA method. Keywords: Syphilis, ELISA, CLIA, blood donation, prevalence