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CODIS STR LOCI (CSF1PO, THOI, TPOX, vWA) GENETIC VARIATION ANALYSIS IN MADURESE Yudianto, Ahmad; Sosiawan, Agung; Margaret, Nola
Folia Medica Indonesiana Vol. 52 No. 1 (2016): JANUARY - MARCH 2016
Publisher : Universitas Airlangga

Endogamy continues to occur among the Madurese people in rural areas of the island of Madura, especially those areas of the smallest islands around the mainland of Madura. Endogamy as seen from a genetic standpoint will increase the frequency of homozygote genotypes. With regard to genetic variations, STRs of nuclear DNA and polymorphisms in mtDNA are frequently examined. Genetic variations in human undergo an evolutionary process through the accumulation of changes in DNA sequence, i.e. through the process of nucleotide substitutions that evolves in number with the directional development of lineage. So far, the genetic variations among the populations in Madura Island have not been known. The present study was an observational analytical research with the purpose of determining the genetic variations in STR CODIS in the populations of Madura Island. Results indicated that, based on loci alelle: CSF1PO, THOI, TPOX, and vWA, there was homozygote genotypes. The allele variations is not specific for Madurese ethnic but this variations may represent married model in Madurese ethnic. According to Mustama (2007), a gene pool is not only a collection of genes but a dynamic system organized and containing the past history of a population. Any genetic information has certain historical, anthropological and statistical aspects necessitating an interdisciplinary coordination and collaboration.

NUCLEOTIDE VARIANCE OF MITOCHONDRIAL DNA D-Loop 126 bp (nt: 34-159) REGION IN MADURESE Sulistyorini, Nilly; Yudianto, Ahmad; Margaret, Nola
Folia Medica Indonesiana Vol. 52 No. 2 (2016): APRIL - JUNE 2016
Publisher : Universitas Airlangga

Endogamy continues to occur among the Madurese people in rural areas of the island of Madura, especially those areas of the smallest islands around the mainland of Madura. Endogamy as seen from a genetic standpoint will increase the frequency of homozygous genotypes. With regard to genetic variations, STRs of nuclear DNA and polymorphisms in mtDNA are frequently examined. Mitochondrial variations in the human undergo an evolutionary process through the accumulation of changes in DNA sequence, i.e. through the process of nucleotide substitutions that evolves in number with the directional development of lineage. So far, the genetic variations among the populations in Madura Island have not been known. The present study was an observational analytical research with the purpose of determining the genetic variations in the polymorphisms of 126-bp mtDNA D-Loop HV2 (nt: 34-159) in the populations of Madura Island. Results indicated that, based on the homology analysis with rCRS sequence, there were 9 variants consisting of two transition mutations, 6 transversion mutations, and one insertion mutation. This indicates that a transversion mutation had a higher probability than transition and insertion mutations. According to Mustama (2007), a gene pool is not only a collection of genes but a dynamic system organized and containing the past history of a population. Any genetic information has certain historical, anthropological and statistical aspects necessitating an interdisciplinary coordination and collaboration.

ANALYSIS OF EARPHONE SWAB MITOCHONDRIAL DNA AS AN ALTERNATIVE MATERIAL FOR IDENTIFICATION EXAMINATION Yudianto, Ahmad; Sispitasri, Yeti Eka; Margaret, Nola
Folia Medica Indonesiana Vol. 52 No. 3 (2016): JULY - SEPTEMBER 2016
Publisher : Universitas Airlangga

Identification include fingerprint, property, medical, dental, serologic and exclusion methods. In the development, identification methods led to molecular forensics, a new field of science evolving since the 1980s, known as DNA fingerprinting. Specimens widely used in DNA assay for identification are blood spots/bloods, semen spots, vaginal swabs, buccal swabs and bones. In addition to these specimens, the last objects often used by the perpetrators/victims can be used, such as hearing aids (headsets/earphones). In its use, earphones are attached to the outer ear skin; thus, the earwax is suspected to adhere to the device. To date, in Indonesia personal identification is performed through swabs of earphones/headsets using the DNA profiling method. In particular, mitochondrial DNA has not been widely used for identification. The present study was of laboratory experimental. Earphones which have been used for 3 days were placed in room temperature for 1, 7, 14 and 20 days. Results showed that the environmental factor of exposure duration had an effect of a significant decrease in the levels of DNA from day 1 to day 20. Only 126-bp mtDNA (HVS II) was detected on the samples of day 1 and continued with sequencing. Mitochondrial DNA has better durability and relatively higher number of copies than those of nuclear DNA. This leads to greater possibility of success in amplification, given the higher number of mitochondrial DNA copies and the fact that mitochondrial DNA is a single locus that allows recombination.

EFFECT OF ROOM TEMPERATURE ON THE QUALITY OF DNA FROM EARPHONE SWAB BY OBSERVING MITOCHONDRIAL DNA [mtDNA] D-LOOP REGION OF 126 bp (HVS II, nt 34-159) AND 143 bp (HVS I, nt 16268-16410) Yudianto, Ahmad; Margaret, Nola
Folia Medica Indonesiana Vol. 53 No. 2 (2017): JUNE 2017
Publisher : Universitas Airlangga

The objects contained in crime scene and its surroundings have significance in the examination of forensic identification. The most commonly used specimens in the examination for identification are blood/blood spots, semen patches, vaginal swabs, buccal swabs and bones, including items often used by the perpetrator/victim the last time. For example, a mobile phone hearing aid (headset/earphone). In the use of earphones attached to the outer ear skin so it is suspected there is a serumen attached to the tool. One factor that can affect the quality of DNA is the prolonged exposure. Until now in Indonesia the effect of long exposure to room temperature on the quality of DNA on the DNA material of earphone swabs through DNA analysis has not been widely known. The type of study was laboratory experimental. Used earphones are exposed at room temperature within 1, 7, 14 and 20 days. The results of this study indicate that the environmental effect, ie the duration of exposure, affects the decrease in DNA content significantly from day 1 to 20. Detection of PCR mtDNA D-Loop HVS I visualization results 143 bp nt: 16268 16410 shows positive detection results (+) Only at day 1 exposure to room temperature [4 sample/66,67%] and day 7 [3 sample/50%]. Visualization of PCR results mtDNA D-Loop HVS II 126bp nt: 34 -159 was performed on day 1 day room exposure [2 sample/33,37%] and day 7 [6 sample/100%]. Conclusion, the duration of exposure to room temperature affect the quality of DNA from earpiece swab material. Decreased levels of earphones DNA swabs showed a significance value (p <0.005) against the effect of long time exposure to room temperature.

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