Claim Missing Document
Check
Articles

Found 26 Documents
Search

ROLE OF BREAK CLUSTER REGION (BCR) - ABELSON MURINE LEUKIMIA (ABL) EXAMINATION IN CHRONIC MYELOGENOUS LEUKEMIA (CML) Sosiawan, Agung
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 2 (2014)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Chronic myelogenous leukemia (CML) is a clonal bone marrow stem cell disorder associated with a characteristic chromosomal translocation called the Philadelphia chromosome which caused a proliferation of mature granulocytes (neutrophils, eosinophils and basophils) and their precursors, increasing unregulated growth of predominantly myeloid cells in the bone marrow and its accumulation in the blood. As myeloproliferative disease, Chronic Myelogenous Leukemia or CML is a malignancy of the sixth-highest, reaching 15% of all blood malignancies in adults with an incidence of 1.1 per 100,000 population (Ugroseno, 2012). The CML diagnosis is made based on a presence of Philadelphia chromosome due to the existence of a reciprocal translocation of chromosomes 9 and chromosome 22 t (9.22), and raises the fusion of Break Cluster Region (BCR) gene of chromosome 22 on band q11 by Abelson Murine Leukemia (ABL). The fused BCR-ABL gene has BCR sequences of different length, so it produces a protein that has a different molecular weight. Despite having different length of BCR sequences, however, the length of fuses ABL gene sequence is constant. Associated with this different BCR sequence length are the three variations of the BCR-ABL gene fusion. The first variation is a Major Break Cluster (M-BCR), the BCR gene break is found in exon 2 in e13-E14 region. This type of CML is the fusion of BCR exon b2 or b3 to ABL exon a2, forming two major transcripts of the b2a2 or b3a2, which has a protein product with 210 kD weight or referred to as p210. The second variation is Minor BCR (m-BCR), which has e1a2 fusion. CML with BCR-ABL gene fusion of this type has a protein product with a molecular weight of 190 kDa or called p190. The third variation is micro-BCR (m-BCR), with BCR gene break between exons E19 and e20b that form mRNA transcripts e19a2, with BCR-ABL protein P230. This fused gene can be detected with qualitative multiplex PCR.
Identification of Single Nucleotide Polymorphism on Bone Morphogenetic Protein 2 Gene in Non-Syndromic Cleft Lip/ Palate Patient Mala Kurniati; RM Coen Pramono D; Agung Sosiawan; David Sontani Perdanakusuma; Hari Basuki Notobroto; Andra Rizqiawan
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16954

Abstract

Cleft lip/palate (CL/P) is one of the most common birth defects in humans. Haploinsufficiency in genesBone Morphogenetic Protein (BMP) 2 is thought to play an important role in the incidence of CL/P. Thisstudy aimed to identify changes in the nucleotide (Single Nucleotide Polymorphism/SNP) BMP 2 rs235768A>T gene in CL/P patient in Indonesia. Seventy samples of DNA that were successfully amplified andrestricted consisted of patient and control samples with the three of which were used for sequencing. Basedon the analysis using restriction enzymes and Finch TV and Bioedit software programs, this study identifieda change from nucleotide A to nucleotide T which is a mutation missense (Serine-Arginine/TCA-TCT).Based on the results of the Fisher’s exact test, there was difference in genotype frequency between the CL/Pgroup and the control. Meanwhile, there was no difference in allele frequencies between the two groups. Theallele frequency T has a higher value than the frequency of the allele A.
The Genetic Aspect of Non-Syndromic Cleft Lip and Palate towards Candidate Genes in the Etiology : A literature Review Mala Kurniati; RM Coen Pramono D; Agung Sosiawan; Ni Wayan Tirtaningsih
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14935

Abstract

Cleft lip and cleft palate (CL/P) is a cleft lip deformity indicated by an opening or an uncommon cleft inthe lip or roof of the mouth (palate). The differences in ethnicity, gender, and the correlation with geneticfactors influence the prevalence of Non-syndromic CL/P. This study was conducted through a literaturereview on genes that were allegedly associated with Non-syndromic CL/P. Genetics play a role, to a greateror lesser extent, in all diseases. Besides, palatogenesis involves many diverse genes in a complex process.In this case, oral cleft phenotypes develop when this process is disrupted in some manner because of genedysfunction. Various genetic approaches, including genome-wide and candidate gene association studiesas well as linkage analysis, have been undertaken to identify etiologic factors, but results have often beeninconclusive or contradictory. Therefore, it concludes that the genetic basis of CL/P is still controversialbecause of the genetic complexity of clefting
The Genetic Aspect of Non-Syndromic Cleft Lip and Palate towards Candidate Genes in the Etiology : A literature Review Mala Kurniati; RM Coen Pramono D; Agung Sosiawan; Ni Wayan Tirtaningsih
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15859

Abstract

Cleft lip and cleft palate (CL/P) is a cleft lip deformity indicated by an opening or an uncommon cleft in the lip or roof of the mouth (palate). The differences in ethnicity, gender, and the correlation with genetic factors influence the prevalence of Non-syndromic CL/P. This study was conducted through a literature review on genes that were allegedly associated with Non-syndromic CL/P. Genetics play a role, to a greater or lesser extent, in all diseases. Besides, palatogenesis involves many diverse genes in a complex process. In this case, oral cleft phenotypes develop when this process is disrupted in some manner because of gene dysfunction. Various genetic approaches, including genome-wide and candidate gene association studies as well as linkage analysis, have been undertaken to identify etiologic factors, but results have often been inconclusive or contradictory. Therefore, it concludes that the genetic basis of CL/P is still controversial because of the genetic complexity of clefting.
Analisis heteroplasmy DNA mitokondria pulpa gigi pada identifikasi personal forensik (Heteroplasmy analysis of dental pulp mitochondrial DNA in forensic personal identification) Ardyni Febri K; Retno Pudji Rahayu; Agung Sosiawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 3 (2013): September 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.813 KB) | DOI: 10.20473/j.djmkg.v46.i3.p130-134

Abstract

Background: Mitochondrial DNA (mtDNA) sequence analysis of the hypervariable control region has been shown to be an effective tool for personal identification. The high copy and maternal mode of inheritance make mtDNA analysis particularly useful when old samples or degradation of biological samples prohibits the detection of nuclear DNA analysis. Dental pulp is covered with hard tissue such as dentin and enamel. It is highly capable of protecting the DNA and thus is extremely useful. One of the diasadvantages of mitochondrial DNA is heteroplasmy. Heteroplasmy is the presence of a mixture of more than one type of an organellar genome within a cell or individual. It can lead to ambiguity in forensic personal identification. Due to that, the evidence of heteroplasmy in dental pulp is needed. Purpose: The study was aimed to determine the heteroplasmy occurance of mitocondrial DNA in dental pulp. Methods: Blood and teeth samples were taken from 6 persons, each samples was extracted with DNAzol. DNA samples were amplified with PCR and sequencing to analyze the nucleotide sequences polymorphism of the hypervariable region 1 in mtDNA and compared with revised Cambridge Reference Sequence (rCRS). results: The dental pulp and blood nucleotide sequence of hypervariable region 1 mitochondrial DNA showed polymorphism when compared with rCRS and heteroplasmy when compared between dental pulp with blood. Conclusion: The study showed that heteroplasmy was found in mithocondrial DNA from dental pulp.latar belakang: Analisis sekuens DNA mitokondria (mtDNA) regio kontrol hypervariable telah terbukti menjadi alat efektif untuk identifikasi personal. Kopi DNA yang banyak dan pewarisan maternal membuat analisis mtDNA sangat berguna ketika sampel lama atau sampel biologis yang terdegradasi menghambat deteksi analisis DNA inti. Pulpa gigi terlindung jaringan keras seperti dentin dan enamel. Hal ini membuat pulpa mampu melindungi DNA dan dengan demikian sangat berguna untuk identifikasi. Salah satu kekurangan DNA mitokondria adalah heteroplasmy. Heteroplasmy adalah adanya campuran lebih dari satu jenis genom dalam sel atau individua. Hal ini dapat menyebabkan ambiguitas pada identifikasi pribadi forensik. Oleh sebab itu, identifikasi personal menggunakan pulpa gigi harus memperhatikan kejadian heteroplasmy. tujuan: Penelitian ini bertujuan untuk meneliti kejadian heteroplamy DNA mitokondria pada pulpa gigi. Metode: Sampel darah dan gigi diambil dari 6 orang, masing-masing sampel diekstraksi dengan metode DNAzol. Sampel DNA diamplifikasi dengan PCR dan sequencing untuk menganalisis polimorfisme urutan nukleotida di hypervariable region 1 mtDNA dan dibandingkan dengan revised Cambridge Reference Sequence (rCRS). hasil: Sekuens nukleotida pulpa gigi dan darah daerah pada hypervariable region 1 DNA mitokondria menunjukkan polimorfisme bila dibandingkan dengan rCRS dan heteroplasmy bila dibandingkan antara pulpa gigi dengan darah. Simpulan: Penelitian ini menunjukkan bahwa heteroplasmy dapat ditemukan pada DNA mitokrondia pulpa gigi.
Identifikasi bite marks dengan ekstraksi DNA metode Chelex (Bite marks identification with Chelex methods in DNA extraction) Imelda Kristina Sutrisno; Ira Arundina; Agung Sosiawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 2 (2013): June 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.296 KB) | DOI: 10.20473/j.djmkg.v46.i2.p107-112

Abstract

Background: In the case of crime often encountered evidence in bite marks form that was found on the victim’s body. Generally, bitemarks identification use standard techniques that compare the interpretation picture with the tooth model of suspected person. However, sometimes the techniques do not obtain accurate results. Therefore another technique is needed to support the identification process,such as DNA analysis that use the remaining epithelium attached in saliva to identify the DNA of the suspected person. In this processes a limited DNA material could be met, not only less in quantity but also less in quality. Chelex known as one of an effective DNA extraction method in DNA forensic case is needed to overcome this problem. Purpose: The study was aimed to examine the use of Chelex as DNA extraction method on a bitemarks sample models. Methods: The blood and bitemarks of 5 persons with were taken. The DNA of each subject was exctracted with Chelex and quantified the quantity with UV Spechtrophotometer. The DNA results was amplified by PCR at locus vWA and TH01 then vizualised by electrophoresis. Results: The electrophoresis’s results showed band at locus vWA and TH01 for blood sample and bite marks with no significant differences. Conclusion: The study showed that Chelex method could be use to extract DNA from bitemarks.Latar belakang: Dalam kasus kejahatan sering dijumpai bukti dalam bentuk bekas gigitan (bitemarks) yang ditemukan pada tubuh korban. Umumnya, untuk mengidentifikasi bite marks menggunakan teknik standar yaitu membandingkan foto interpretasi dengan model gigi dari orang yang dicurigai. Namun demikian teknik ini terkadang tidak mendapatkan hasil yang akurat, sehingga diperlukan teknik lain untuk menunjang keberhasilan proses identifikasi pelaku, yakni melalui analisis DNA bitemarks, yang diperoleh dari saliva yang mengandung sisa epitel tersangka pelaku. Sampel DNA yang berasal dari bitemarks umumnya terbatas, tidak hanya terbatas dalam kuantitas tetapi juga terbatas dalam kualitas. Hal ini seringkali menimbulkan kesulitan tersendiri dalam proses analisisnya. Chelex yang dikenal sebagai salah satu metode ekstraksi yang efektif di bidang forensik, sangat diperlukan untuk mengatasi kendala tersebut . Tujuan: Penelitian ini bertujuan untuk meneliti penggunaan metode ekstraksi DNA metode Chelex pada sampel bite marks. Metode: Darah dan cetakan gigi dari 5 subjek diambil, dan DNA di ekstraks dengan Chelex dan kemudian diuji kuantitas dengan UV Spechtrophotometer. Setelah itu hasil diamplifikasi dengan PCR pada lokus vWA dan TH01 kemudian divisualisasi dengan elektroforesis. Hasil: Hasil elektroforesis menunjukkan adanya band pada lokus vWA dan TH01 untuk sampel darah dan cetakan gigi tanpa perbedaan yang signifikan secara statistika. Simpulan: Penelitian ini menunjukkan bahwa metode Chelex dapat digunakan untuk mengekstraksi DNA dari bite marks.
Effectiveness of Line communication application as a social media on changes in tooth brushing behavior of junior high school students in Banjarmasin Widodo Widodo; Darmawan Setijanto; Agung Sosiawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 4 (2016): December 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.596 KB) | DOI: 10.20473/j.djmkg.v49.i4.p223-228

Abstract

Background: There were only 10.7% of junior school students in Banjarmasin brushing their teeth before bedtime. Using Line (as one of the social media) can be assumed as an effective strategy to spread information. Purpose: This study aimed to reveal changes in tooth brushing behavior before bedtime in students of class VII in all state junior high schools in Banjarmasin after receiving information disseminated through Line. Method: Pre and post test technique with control group design was used in this research. Result: One week before the treatment, the mean frequency of tooth brushing behavior before bedtime in the Line group was 1.90, while in the poster group was 1.93. During the treatment, the mean frequency of tooth brushing behavior before bedtime in the Line group was 4.78 in the first 7 days, 5.07 in the second week, and 5.67 in the third week. On the other hand, the mean frequency of tooth brushing behavior before bedtime in the poster group was 4.66 in the first 7 days, 4.61 in the second week, and 5.18 in the third week. Conclusion: Messages/ information disseminated through both of Line and poster can give a significant change in tooth brushing behavior before bedtime. Nevertheless, Line can trigger better effectiveness than poster in stimulating a change in tooth brushing behavior before bedtime.
The role of family history as a risk factor for non-syndromic cleft lip and/or palate with multifactorial inheritance Agung Sosiawan; Mala Kurniati; Coen Pramono Danudiningrat; Dian Agustin Wahjuningrum; Indra Mulyawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 2 (2021): June 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i2.p108-112

Abstract

Background: Cleft lip with or without cleft palate (CL/P) is a facial growth ‘disorder that occurs during gestation and has multifactorial causes owing to both genetic and environmental factors. Several factors can increase the likelihood of CL/P, and one of them is family history. Differences in results obtained from studies conducted across several countries concerning family history as a risk factor for CL/P suggest there is no consensus on how the condition is inherited. Purpose: This study aims to review the literature on the role of family history as a risk factor contributing to the incidence of non-syndromic CL/P (NSCL/P). Review: This review discusses the etiology of CL/P and the risk factors influencing the incidence of CL/P. The review also examines the criteria for inheriting multifactorial disorders to calculate the risks involved should there be a recurrence of the condition based on family history. Conclusion: CL/P is a type of multifactorial disorder with unclear etiology. Therefore, it is important to investigate the risk factors stemming from family history (which play an important role) related to the recurrence risk. Additionally, there should be focus on increasing genetic education and offering counselling to parents and pregnant women.
Six Commandments of Treatment Protocols During Covid-19 Pandemic in Dentistry Agung Sosiawan; Dian Agustin Wahjuningrum; Anuj Bhardwaj; Kratika Mishra; Sheetal Khandelwal; Amit Bhardwaj; Shivani Bhardwaj
Biomolecular and Health Science Journal Vol. 4 No. 1 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i1.27427

Abstract

In December 2019, an outbreak in the city of Wuhan, Hubei China occurred and was named COVID- 19 by the World Health Organization and declared as a pandemic on January 30, 2020. The etiologic agent of acute respiratory disease is the novel corona virus 2019 (2019-nCoV) or the extreme acute respiratory syndrome corona virus 2 (SARS-CoV-2). During many dental operations, aerosols are produced and these aerosol methods, which are major problems for dentists, have become the main area of concern in dentistry. Dentists are at greatest risk since they are located close to the oropharynx. This study summarizes the effect of coronavirus disease 2019 (COVID-19) on dental care, administration, and emergency dental treatment service. It also identifies COVID-19 and explains about six commandments for dental practitioners during the treatment process to be practiced during the pandemic.
Gigi Sehat: Channel Video Edukasi Kesehatan Gigi dan Mulut untuk Orangtua Murid di Surabaya Aulia Ramadhani; Agung Sosiawan
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 5 NOMOR 2 SEPTEMBER 2021 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.874 KB) | DOI: 10.30595/jppm.v5i2.6077

Abstract

Karies gigi merupakan salah satu masalah kesehatan gigi dan mulut yang paling banyak ditemukan pada anak. Hal ini berdasarkan penelitian yang dilakukan di Surabaya pada anak usia pra-sekolah menunjukkan prevalensi karies gigi yang tinggi (67.5%) dengan tingkat keparahan yang juga tinggi (def: 7.0). Hal ini dapat disebabkan karena rendahnya pengetahuan ibu terkait kesehatan gigi dan mulut. Rendahnya pengetahuan tersebut disebabkan oleh rendahnya kemauan ibu untuk mengakses informasi kesehatan gigi dan mulut yang berdampak pada perawatan kesehatan gigi anak. Berdasarkan hasil penelitian epidemiologi yang dilakukan oleh tim FKG Unair, Salah satu media yang paling banyak digunakan oleh para ibu di Surabaya dalam mengakses informasi di internet adalah aplikasi YouTube, dengan prevalensi sebesar 46.2%. Selain itu didukung pula dengan persentasi ibu-ibu yang memiliki smartphone dengan akses internet di Surabaya sangat tinggi yaitu sebesar 91.6%. Kami menawarkan solusi pembuatan video edukasi yang merupakan media yang cukup diminati ibu wali murid di wilayah Keputih dengan tujuan untuk menyediakan akses informasi pada masyarakat Keputih tentang kesehatan gigi dan mulut.  Bermitra dengan Puskesmas Keputih, Surabaya, program sosialisasi ini diikuti oleh 30 orang wali murid SDN Medokan Semampir 1. Metode pelaksanaan program ini adalah dengan penyebaran link video yang akan diunggah di aplikasi YouTube. Video edukasi kesehatan gigi dan mulut dibuat berseri dan diunggah setiap minggunya di kanal YouTube Gigi Sehat. Dari kanal YouTube tersebut, akan dievaluasi jumlah penonton tiap minggunya. Sosialisasi video diawali dengan pemberian pre-test dan post-test mengenai kesehatan gigi dan mulut. Dari 30 partisipan yang hadir, didapatkan lebih dari 75% responden mengalami peningkatan skor post-test. Jumlah penonton video juga mengalami peningkatan tiap videonya. Dari peningkatan pengetahuan, jumlah penonton, serta respon positif terhadap video ini, dapat disimpulkan bahwa program pemberdayaan masyarakat ini berhasil.
Co-Authors Adla, Karina Awanis Afidati, Yasinta Izzah Ahmad Yudianto Ahmad Yudianto Alexander Patera Nugraha Amalia, Rovilla Amarilda, Faila Shofa Amit Bhardwaj An'nisaa Chusida Andiyani, Intan Andra Rizqiawan Andry Sentosa Anjani, Nindya Rizki Anjani, Nindya Rizqi Anuj Bhardwaj Ardyni Febri K Arif Rahman Nurdianto Arofi Kurniawan Aryo Dwipo Kusumo Aspalilah Alias Atik Kurniawati Aulia Ramadhani Azhar, Imam Safari Beshlina Fitri Widayanti Roosyanto Prakoeswa Beta Novia Rizky David Sontani Perdanakusuma Detalicia, Callista Gladys Fionna Devina, Dida Dhywinanda, Devin Elysia Dian Agustin Wahjuningrum Dini Parasila Dwi Susanti Fadila, Vivin Dwi Fajariana Fitriani Fatah, Rizki Amelia Fauzan Fauzan Fery Setiawan Fissando Yanuar Muhammad Giftania Nuri Susilo Gilang Rasuna Sabdho Wening Hadinata, Daniel Hari Basuki Notobroto Heni Puspitasari Hidayanto, Agung Ridwan Hutapea, Yohana Vita Melodi Imelda Kristina Sutrisno Ira Arundina Jordana, Jesslyn Kamilah, Tsaniya Karimah, Zalfa Khotimah, Fridaniyanti Khusnul Kratika Mishra Krissanti, Theresa Dian Kumala Dewi, Ni Nyoman Astari Kurniati, Mala Kuswanda, Crisna Tri Laisyaina, Defita Ayu Lutfi Malia Rossa Maharani, Gita Alethea Kristi Mahiswari, Diyang Mala Kurniati Mala Kurniati Margaret, Nola Maria Istiqomah Marini Muhammad Halim, Muhammad Mumtaz Ramadhani Putra Pesat Gatra Naila Azifatur Rahmat Najminoor Ramadhani Ridlo Ni Wayan Tirtaningsih Ninuk Hariyani Nugroho, Sepdhyo Wahyu Nuradiyah, Fanny Nurrezeki, Andi Adani Pande, I Ketut Brahma Pangestika, Elvira Widya Pramita, Anggi Pratama, Innocencio Kresna Priyanridania, Erika Putri Gofur, Aisyah Rachmadani R. Darmawan Setijanto Rachmaningayu, Ulyasari Rachmawati, Almira Ramadhani, Maya Eka Regina, Veronica Retno Palupi Retno Pudji Rahayu RM Coen Pramono RM Coen Pramono D Romadhona, Widya Rizky Roselle, Veronica Regina Rumokoi, Rauhansen Bosafino Salim, Jennifer Fiona Salsabila Maghfirani Santosa, Lintang Maudina Saputra, Benny Sembadani, Yuliana Merlindika Sengupta, Kaushik Setiyowati, Dini Setyabudi Setyabudi Sheetal Khandelwal Shivani Bhardwaj Sholihah, Naimatus Sholihah, Nimatus Sudiartha, Nancy Cynthia Swadheena Patro Syahdryani, Zhafira Putri Syarifah, Indi Mazaya Taufan Bramantoro Thomas Reynaldi Winanto Titiek Berniyanti Tito Krisna Gianosa Vera Rimbawani Sushanty Vita Ariesta Angesti Wardi, Ully Nafisah Widodo Widodo Yani Corvianindya Rahayu Yuli Dwi Kristanti Yusticia, Ica Zainul Cholid Zuhdi, Nadya Adina