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Pengaruh Pemberian Virgin Coconut Oil (VCO) dan Olive Oil terhadap Mikroanatomi Ren Tikus Putih (Rattus norvegicus) Nurasri, Riani; Wachidah Yuniwarti, Enny Yusuf; Djaelani, M Anwar
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.651 KB) | DOI: 10.14710/bioma.20.2.133-139

Abstract

Virgin Coconut Oil (VCO) and olive oil have been widely applied in the field of health. Saturated fatty acid content of VCO dominates the total fatty acid content of about 85%. Olive oil contains a lot of oleic acid, which is one type of unsaturated fatty acid (mono-unsaturated fatty acid). This study aimed to analyze the kidney microanatomy rat (Rattus norvegicus) after supplementation with VCO and olive oil in the feed. This study used 25 rats were divided into five treatment groups, P1 was the control group, P2 was a group with VCO 5 mL / kg feed, P3 was a group with VCO 10 mL / kg of feed, P4 was a group with olive oil 5 mL / kg feed and was a group with olive oil 10 mL / kg of feed. The results of ren weight and diameter rat glomerular analyzed using analysis of variance (ANOVA). Research design used was completely randomized design with five treatments and five replication. Results of studies on weight ren showed no significant difference between treatments P2, P3, P4 and P5 to the control (P1). The results of the glomerular diameter  shows there is significantly difference between the diameter of the glomerulous treatment P2 and P3 with control (P1), while in treatment P4 and P5 are not significantly different from the control so that it can be concluded that the administration of the VCO and olive oil at various concentrations do not give rise to different responses to weight ren. Olive oil does not increases glomerular diameter white mice, while VCO at a dose of 5 mL / kg feed and 10 mL / kg of feed can increased glomerular diameter mice. Keywords: ren weight, glomerular diameter, Virgin Coconut Oil (VCO), olive oil.
Glikogen Otot Rangka Ayam Broiler (Gallus gallus) setelah Pemberian Teh Kombucha dalam Air Minum Fathoni, Nasrul; Djaelani, M Anwar; Isdadiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.725 KB) | DOI: 10.14710/bioma.20.2.78-85

Abstract

Kombucha tea beverage obtained by fermenting sweetened green tea for 12 days with Acetobacter xylinum and Saccharomyces which produce various kinds of organic acids, vitamins, and acts as a probiotic. The role of kombucha tea as a growth promoter is to that improve the metabolic process in the digestion of broiler chickens, so that nutrients can and fulfilled optimally for growth and development. The aim of this research was to analyze the sceletal muscle glycogen glycogen (Gallus gallus) after treat with kombucha tea in drinking water. Kombucha tea used is the result of fermentation of green tea for 12 days. This research used DOC chickens (Day Old Chicken) as much as 20 randomly divided into 4 treatments with concentration ie 0%, 10%, 20%, and 40% kombucha tea in drinking water for 32 days. The variables measured were skeletal muscle glycogen levels, body weight, feed consumption and drink consumption. The data obtained were analyzed using ANOVA followed by Duncan Test with 95% confidence level using SPSS 17.0 software. The results of this study showed that kombucha tea on skeletal muscle glycogen and feed consumption showed no significant difference, while on body weight and drink consumption showed significantly different results. Based on the results of the study, it can be concluded that giving kombucha tea in drinking water to a concentration of 40% has not been able to affect the skeletal muscle glycogen in broiler chickens. Key words: Kombucha tea, broiler chicken, glycogen skeletal muscle
Struktur Mikroanatomi Duodenum Puyuh (Coturnix coturnix japonica) Setelah Pemberian Tepung Daun Kelor (Moringa oleifera Lam.) Dalam Pakan Umar, Ja'far; Djaelani, M Anwar; Kasiyati, Kasiyati; Sunarno, Sunarno
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.31493

Abstract

AbstrakKelor merupakan tanaman tropis yang kaya fitonutrien dan senyawa bioaktif. Kandungan pada daun kelor mampu mendukung proses pencernaan, absorpsi, metabolisme, dan produktivitas unggas. Pemberian tepung daun kelor dalam pakan dengan konsentrasi yang tepat dapat mengefektifkan proses pencernaan dan absorpsi nutrien dengan memperbaiki struktur mikroanatomi duodenum puyuh. Penelitian ini bertujuan menganalisis pemberian tepung daun kelor dalam pakan terhadap diameter lumen, tinggi vili, lebar vili, dan tebal muskular duodenum puyuh. Desain penelitian menggunakan RAL dengan 5 perlakuan dan 3 kali ulangan. Perlakuan konsentrasi pemberian tepung daun kelor dalam pakan meliputi 0, 2,5; 5; 7,5, dan 10%. Preparat histologi dibuat melalui metode parafin dengan pewarnaan Hematoksilin-Eosin (HE). Parameter yang diamati meliputi diameter lumen, tinggi vili, lebar vili, dan tebal lapisan muskular duodenum. Data dianalisis menggunakan ANOVA dilanjutkan dengan uji Duncan menggunakan program SPSS. Hasil penelitian menunjukkan bahwa tepung daun kelor dalam pakan berpengaruh tidak nyata  (P >0,05) terhadap diameter lumen, tinggi vili, dan lebar vili, namun berpengaruh nyata (P <0,05) terhadap tebal lapisan muskular pada duodenum puyuh.  Simpulan dari penelitian ini bahwa pemberian tepung daun kelor dalam pakan tidak berpotensi mengubah mikroanatomi duodenum puyuh sehingga dapat dimanfaatkan sebagai aditif pakan. Namun, pemberian tepung daun kelor dalam pakan berpotensi dalam meningkatkan ketebalan lapisan muskular duodenum puyuh.AbstractMoringa is a tropical plant rich in phytonutrients and bioactive compounds. The content in moringa leaves can support the process of digestion, absorption, metabolism, and productivity of poultry. Giving moringa leaf meal in feed with the right concentration can streamline the digestive process, and nutrient absorption by improving the microanatomical structure of the quail duodenum. This study aims to analyze the provision of moringa leaf flour in feed on the diameter of the lumen, villi height, villi width and muscular thickness of the quail duodenum. The research design used RAL with 5 treatments and 3 replications. The treatment concentration of moringa leaf meal in feed included 0; 2.5; 5; 7.5, and 10%. Histology preparations were made through paraffin method with Hematoxylin-Eosin (HE) staining. Parameters observed included lumen diameter, villi height, villi width, and duodenal muscular layer thickness. Data were analyzed using ANOVA followed by Duncan's test using the SPSS program. The results showed that moringa leaf meal in feed had no significant effect (P >0.05) on lumen diameter, villus height and villus width, but had a significant effect (P <0.05) on the thickness of the muscular layer in the duodenum of quail. The conclusion of this study is that the provision of moringa leaf meal in feed does not have the potential to change the microanatomy of the quail duodenum so that it can be utilized as a feed additive. However, the provision of moringa leaf meal in feed has the potential to increase the thickness of the muscular layer of the quail duodenum.