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All Journal Wahana : Tridarma Perguruan Tinggi Journal Pharmasci (Journal of Pharmacy and Science) Folia Medica Indonesiana
Amilah, Susie
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing

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Efektivitas Ekstrak Rimpang Pacing (Costus speciosus), Daun Srikaya (Annona squamosa L.) dan Ekstrak Kombinasinya Terhadap Penurunan Jumlah Folikel Tersier dan Folikel De Graff Pada Mencit Betina (Mus musculus) Ajiningrum, Purity Sabila; Amilah, Susie; Widyaningtyas, Prafikka Galuh
Journal of Pharmacy and Science Vol. 5 No. 1 (2020): Journal of Pharmacy and Science
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v5i1.164

Abstract

Salah satu cara menanggulangi pertumbuhan penduduk yang tidak terkendali adalah dengan penggunaan kontrasepsi melalui program Keluarga Berencana (KB). Kontrasepsi yang beredar kebanyakan merupakan kontrasepsi sintetis yang memiliki risiko terhadap kesehatan pemakainya. Alternatif lain yang dapat digunakan sebagai kontrasepsi dengan efek samping tidak berbahaya yaitu memanfaatkan tanaman obat yang mengandung senyawa antifertilitas. Beberapa tanaman yang mengandung senyawa antifertilitas yaitu srikaya (Annona squamosa L.) dan pacing (Costus speciosus (Koen.). Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak rimpang pacing dan daun srikaya terhadap jumlah folikel tersier dan folikel de graff pada mencit betina. Penelitian ini adalah penelitian eksperimental menggunakan Metode analisis uji F (anova) satu arah dengan percobaan rancangan acak lengkap (RAL). Penurunan jumlah folikel tersier terbaik adalah pada pemberian ekstrak daun srikaya pada dosis 250 mg/kg BB. Pada folikel de graff, perlakuan terbaik adalah pada pemberian ekstrak daun srikaya dosis 250 mg/kg BB dan pemberian ekstrak kombinasi ekstrak rimpang pacing dan daun srikaya yaitu pada pemberian dosis 125:125 mg/kg BB. Penurunan jumlah folikel tersier dan folikel de graff juga dapat disebabkan karena adanya kandungan bahan aktif yang ada didalam rimpang pacing dan daun srikaya. Penurunan jumlah folikel pada tiap-tiap perlakuan juga dipengaruhi banyaknya dosis yang diberikan sehingga semakin banyak jumlah dosis yang diberikan, maka semakin sedikit jumlah folikel yang terbentuk.
Effectiveness Test of Juwet Leaf Extract and Juwet Cortex Against Desreasing Blood Glucose Levels of Hiperglemic Mice: Uji Efektivitas Ekstrak Daun Juwet dan Ekstrak Kulit Batang Juwet (Syzygium cumini L.) Terhadap Penurunan Kadar Glukosa Darah Mencit (Mus musculus) yang Hiperglikemia Ajiningrum, Purity Sabila; Amilah, Susie; Kurela, Wela Anies
Journal of Pharmacy and Science Vol. 6 No. 2 (2021): Journal of Pharmacy and Science
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v6i2.213

Abstract

Diabetes melitus drugs are currently quite expensive, so they require a cheaper and more effective substitute for drugs to control glucose levels in people with diabetes melitus. Exploring local potentials using medicinal plants can be an alternative to chemical drugs. One of the medicinal plants that is thought to be used for people with diabetes mellitus is juwet. Juwet leaves are suspected to have hypoglycemic activity, because they contain flavonoid and saponin compounds. Juwet bark also contains chemical compounds, namely flavonoids, saponins, and tannins. This study aims to determine the decrease in blood glucose levels in dextrose-induced mice by giving juwet leaf extract and juwet bark extract (Syzygium cumini L.). The test animals used were 36 male mice (Mus musculus) aged 2-3 months with a weight of 20-30 grams. This type of research was carried out experimentally and the design of this study used was Completely Randomized Design (CRD), which was divided into 12 treatments and 3 replications. The 12 treatments are juwet leaf extract and juwet bark with the same dose, namely the dose of 0 mg/kg BB, 150 mg/kg BB, 200 mg/kg BB, 250 mg/kg. Data from the research were analyzed statistically using One Way ANOVA and continued with the LSD test. The results showed that administration of juwet leaf extract and juwet bark extract at various doses had a significant effect on decreasing blood glucose levels of mice. The most effective dose to reduce blood glucose levels is juwet bark extract with a dose of 250 mg/kg BB.
PENGARUH EKSTRAK KULIT DELIMA (Punica granatum) DAN RUMPUT TEKI (Cyperus rotundus) TERHADAP MORTALITAS LARVA NYAMUK Aedes aegypti Binawati, Diah Karunia; Amilah, Susie
WAHANA Vol 63 No 2 (2014)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v63i2.1126

Abstract

Dengue Haemorrhagic Fever (DHF) is nowadays one of the most common public health problems in Indonesia, which lately the number of patients and the range of dissemination tend to be increasing. The most simple and effective way to attempt termination of the life cycle of the mosquito Aedes aegypti is to kill mosquito larvae. Frankly, the act of using arviciding or chemical pesticides often cause these following problems such as environmental pollution, the emergence of resistance and disruption of the ecological balance. The use of plants as a natural pesticide that is environmental -friendly is one of the alternatives that could be done by the society. One of the ways is by using pomegranate peel extract (Punica granatum) and nut-grass extract (Cyperus rotundus). The skin of pomegranate contains saponins, flavonoids and polyphenols, flavonoids and polyphenols. While the nut-grass (Cyperus rotundus) contain alkaloids, flavonoids, cineol, pinene, siperon, rotunal, siperenon, and siperol. This study aims to determine whether there is the effect of giving pomegranate peel extract (Punica granatum) and extract of the nut-grass (Cyperus rotundus) against larvae of Aedes Agypti mortality during a 24-hour treatment. The research design used was a complete randomized design, with pomegranate peel extract treatment (0%, 5%, 10%, 15% and 20%), and extract the nut-grass (0%, 5%, 15% and 20%), each treatment was given 3 repetitions, and observed mortality of larvae of Aedes Agypti after treated for 24 hours. The results showed that 1. There is an effect of pomegranate peel extract against Aedes aegypti larvae mortality for 24 hours, and at a level of 20% has the highest mortality rate (12.5 ± 2.43) 2. There is an effect of nut-grass extract extract against Aedes aegypti larvae mortality for 24 hours, and at a level of 20% has the highest mortality rate (6 ± 1.14) 3. The extract of pomegranate skin has the ability to kill the larvae of Aedes aegypti greater than the extract of nut-grass
EFFECT OF CHERRY LEAF (Muntinga calabora) BIOINSECTICIDES EXTRACT TOWARDS MORTALITY OF WORM SOIL (Agrotis ipsilon) AND ARMYWORM (Spodoptera exiqua) ON PLANT LEEK (Allium fistolum) Binawati, Diah Karunia; Amilah, Susie
WAHANA Vol 61 No 2 (2013)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v61i2.1267

Abstract

Leek plant (Allium fistulosum) that grown at Village Treceh Sajen Mojokerto District Pacetoften suffer from some loss that caused by the attacks from pests such as worm soil (Agrotisipsilon) and armyworm (Spodoptera exiqua). The use of synthetic insecticides is very dangerous,because the residue will affect the health of consumers. Cherry plant (Muntinga calabura) containsactive substances such as tannins, saponins and flavonoids that can be used as bioinsecticides.This study aims to determine whether there is an effect of cherry leaf bioinsecticidesextract towards mortality of worm soil and armyworm in a in vitro manner, and to observe thegrowth of leek plant at harvest scallion age 2 months. The study design used is a CompleteRandomized Design, with 4 treatments using cherry leaf extract (0%, 2.5%, 5% and 7.5%), andgiven 6 replications. Samples of worm soil and armyworm caterpillars each are 120 which itsmortality will be observed for 24 hours. Leek plant sample are 60 plants. The results of dataanalysis showed a 24-hour LC50 value for worm soil is 4.240% and the 24-hour LC50 values forarmyworm is 3,434%. These results indicate that the cherry leaf extract is more effective toannihilate armyworm than it is to annihilate worm soil.To observe the effect of cherry leaf bioinsecticides treatment extract towards the growth ofleek plant leaves were harvested 2 months of age. Results of test F (0.024) indicates that there is asignificant effect of cherry leaf extract towards the growth of leek plants. The results of the LSDanalysis of these data showed that the P3 treatment with a concentration of 7.5% is the one thatproduces the best growth of the plant.
DAYA HAMBAT EKSTRAK SAMBILOTO (ANDROGRAPHIS PANICULATA) DENGAN BERBAGAI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI SALMONELLA TYPHI Amilah, Susie; Prasetyo, Agus
WAHANA Vol 56 No 1 (2011)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v56i1.1391

Abstract

Andrographis paniculata atau yang dikenal dengan nama sambiloto adalah tumbuhan yang biasa dipakai oleh masyarakat untuk mengobati typoid. Tujuan penelitian ini adalah untuk mengetahui daya hambat ekstrak sambiloto terhadap pertumbuhan Salmonella typhi dan mengetahui konsentrasi yang paling efektif dalam menghambat pertumbuhan bakteri Salmonella typhi. Bahan uji yang digunakan adalah biakan bakteri Salmonella typhi yang diisolasi dari penderita yang dirawat di RSU Dr. H. Koesnadi Bondowoso, yang kemudian diuji dengan menggunakan ekstrak sambiloto dengan berbagai konsentrasi. Adapun konsentrasi yang digunakan adalah konsentrasi 5%, 10%, 20%, 30%, dan 40%. Pengamatan jumlah koloni kuman dilakukan dengan metode Total Plate Count (TPC) . Data dianalisis menggunakan uji F yang dilanjutkan dengan LSD. Kesimpulan penelitian ini menunjukkan bahwa Andrographis paniculata atau sambiloto dengan berbagai konsentrasi memiliki kemampuan dalam menghambat pertumbuhan bakteri salmonella typhi. Konsentrasi yang memberikan daya hambat terbaik adalah konsentrasi ekstrak 40%. Dengan demikian masyarakat dapat menggunakan sambiloto sebagai obat alternatif dalam melakukan pengobatan terhadap penderita typoid.
Leaf and Petiole Extract of Centella Asiatica are Potential for Antifertility and Antimicrobial Material Amilah, Susie; Sukarjati, Sukarjati; Rachmatin, Diaz P; Masruroh, Masruroh
Folia Medica Indonesiana Vol. 55, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Ethanol extract of leaves and petiole of pegagan (Centella asiatica) is potential as antimicrobial and antifertility. This study was to investigate whether ethanol extracts of leaf and petiole of Centella asiatica has potential as an antimicrobial and can decrease sperm quality and spermatogenic cells of mice. This was a randomized controlled study using Centella asiatica plant from which we made ethanol extract. The extract of the leaves and petiole of Centella asiatica was subjected to antimicrobial test using the bacteria S. aureus, S. epidermidis, and A. aerogenes. Then the observation was done after a 24-hour-old bacterial cultures by measuring the diameter of the clear zone in mm. Thereafter, the extract was given to randomly grouped mice that were divided into two groups receiving the leaf extract in one group and the petiole exract in another for 35 days. On day 36 the mice were sacrificed for spermatozoa quality and testicular histology examination. Statistical analyses used in this study were ANOVA and LSD tests. There was inhibition zone difference between leaf and petiole (p=0.000). Inhibition zone between bacteria S. epidermidis, S. aureus and A. aerogenes showed no difference (p=0.198). Inter-dose inhibition zone showed difference (p=0.000). Centella asiatica leaf extract showed inhibition zone better than that of Centella asiatica petiole extract. In spermatozoa quality test, there was effect of Centella asiatica leaf extract on sperm motility (p<0.05), viability (p<0.05), morphology (p<0.05) and concentration (p<0.05). No effect of pegagan petiole extract was found on sperm motility (p<0.05), viability (p<0.05), abnormal morphology (p<0.05) and concentration (p<0.05). Highest degradation of spermatozoa quality was obtained from Centella asiatica petiole extract at a concentration of 150 mg/kg bw. Spermatogenic cell test results showed no effect of Centella asiatica leaf extract in decreasing spermatogonia (p=0.000), spermatocytes (p=0.000), and spermatids (p=0.000) counts. No effect of pegagan petiole extract in decreasing the number of spermatogonia, spermatocytes and spermatids. The highest reduction spermatogenic cells of seminiferous tubules was obtained by Centella asiatica petiole extract in a concentration of 150 mg/kg bw. In conclusion: Centella asiatica leaf and petiole extract has antimicrobial potency as contra-ceptives
Role of immunoglobulin g (igg) from the induction of escherichia coli pili adhesion protein isolated from infertile male semen with 32.2 kda molecular weight as opsonin and anti-adhesion an in vitro escherichia coli infection Sukarjati, Sukarjati; Amilah, Susie
Folia Medica Indonesiana Vol. 53, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Escherichia coli (E. coli), a major cause of male genital tract infections, is asymptomatic and may result in male infertility. We have succeeded in isolating and characterizing proteins of E. Pili coli isolates from semen of infertile men who function as adhesin with a molecular weight (MW) 32.2 kDa. This study aims to prove the ability of IgG results adhesion proteins induced pili of E. MW coli 32.2 kDa as opsonin to determine the value of the activity and phagocytic capacity and as an anti- adhesion by calculating the average number of E. coli that attached to human spermatozoa. E. coli infertile men's semen were cultured using standard bacteriology. Peritoneal macrophages were isolated from mice. Spermatozoa from donors were prepared using Sil with Select Plus. IgG was obtained from mice immunized with (1) PBS (control), (2) E. coli pili adhesion protein isolated from infertile men semen with MW of 32.2 kDa and (3) weakened E. coli isolated from infertile men's semen. Phagocytic activity value was determined by counting the number of cells activated macrophage phagocytosis process in 100 cells. Phagocytic capacity value was determined by counting the number of bacteria ingested by 25 macrophages. Anti-adhesion test was done by counting the number of bacteria attached to 100 spermatozoa. The results of this study showed difference (p=0.000) in phagocytic activity and phagocytic capacity (p=0.000) between treatment (1) and (2), and between treatment (1) to (3). However, treatment (2) and (3) did not differ neither in phagocytic activity (p=0.693) nor in phagocytosis capacity (p=0.125). Anti-adhesion test produces difference (p=0.000) in the number of E. coli that attached to human spermatozoa between treatments (1) and treatment (2), and between treatments (1) and (3). The number of E. coli that attached to human spermatozoa between treatment (2) and treatment (3) was not significantly different (p=0.371). In conclusion, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa can increase phagocytic activity and capacity as well as serve as an anti- adhesion. Thus, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa is protective against in vitro E. coli infection, so that it can be used as material to prevent male reproductive tract infections due to E. coli.
Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System Sukarjati, Sukarjati; Amilah, Susie; Sudjarwo, Sudjarwo
Folia Medica Indonesiana Vol. 54, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Escherichia coli (E. coli) is the leading cause of male genital tract infection with no symptoms of infertility. Protein E. coli pili hemagglutinin isolated from infertile male sperm with 32.2 kDa MW acts as adhesion in spermatozoa. This study aimed to prove whether E. coli pili adhesin 32.2 kDa MW is toxic to male reproductive system. Samples consisted of spermatozoa of 30 guinea pigs divided into three groups: control, immunized with E. coli pili adhesin 32.2 kDa MW protein, and transurethral infected E. coli. Observations of sperm motility, vitality and morphology were performed under a microscope. MDA levels and sperm DNA damage were measured by a spectrophotometer and comet assay method and observed using a fluorescent microscope. There was no difference between control and immunization group of E. coli pili adhesin in motility (p=0.499), vitality (p=0.817) and morphology (p=0.176); between control and transuretral infection groups in motility (p=0.000), vitality (p=0.000) and morphology (p=0.000); and between control and both treatment groups in motility (p=0.001), vitality (p=0,000) and morphology (p=0.000). Histologic analysis showed E. coli pili adhesin of 32.2 kDa MW immunization group did not suffer from testicular tissue damage, while the positive group showed a deterioration of seminiferous tubular cells. MDA levels differed between immunization group E. coli pili, transurethral infection group, and control (p=0.024) and between transurethral and control (p=0.007) groups. However, between control and immunized group with E. coli pili protein showed no difference (p=0.251). DNA damage differed (p=0.000) between immunized group with E. coli pili, transurethral infection and control group; between control and transurethral infected group (p=0.000); and between transurethral infection group and E. coli pili protein immunization group (p=0.000). However, between control and E. coli pili immunization group showed no difference (p=0.600). In conclusion, E. coli pili adhesin 32.2 kDa MW protein is not toxic for sperm quality and the quality of sperm molecules.
Co-Authors Afiff, F.E Agus Prasetyo Binawati, Diah Karunia Chandra, M. I. Chusniyah, Lailatul Indarti Indarti Kesumawardhani, A. Kurela, Wela Anies Masruroh MASRUROH Nisak, S.Z Purity Sabila Ajiningrum Rachmatin, Diaz P Sudarwati, C. Sudjarwo Sudjarwo Sugiarti, L. Sukarjati Sukarjati, Sukarjati Tri Novita Sari, Tri Novita Warda, Imro'atu Widyaningtyas, Prafikka Galuh