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Journal : Wahana : Tridarma Perguruan Tinggi

PENGARUH EKSTRAK KULIT DELIMA (Punica granatum) DAN RUMPUT TEKI (Cyperus rotundus) TERHADAP MORTALITAS LARVA NYAMUK Aedes aegypti Binawati, Diah Karunia; Amilah, Susie
WAHANA Vol 63 No 2 (2014)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v63i2.1126

Abstract

Dengue Haemorrhagic Fever (DHF) is nowadays one of the most common public health problems in Indonesia, which lately the number of patients and the range of dissemination tend to be increasing. The most simple and effective way to attempt termination of the life cycle of the mosquito Aedes aegypti is to kill mosquito larvae. Frankly, the act of using arviciding or chemical pesticides often cause these following problems such as environmental pollution, the emergence of resistance and disruption of the ecological balance. The use of plants as a natural pesticide that is environmental -friendly is one of the alternatives that could be done by the society. One of the ways is by using pomegranate peel extract (Punica granatum) and nut-grass extract (Cyperus rotundus). The skin of pomegranate contains saponins, flavonoids and polyphenols, flavonoids and polyphenols. While the nut-grass (Cyperus rotundus) contain alkaloids, flavonoids, cineol, pinene, siperon, rotunal, siperenon, and siperol. This study aims to determine whether there is the effect of giving pomegranate peel extract (Punica granatum) and extract of the nut-grass (Cyperus rotundus) against larvae of Aedes Agypti mortality during a 24-hour treatment. The research design used was a complete randomized design, with pomegranate peel extract treatment (0%, 5%, 10%, 15% and 20%), and extract the nut-grass (0%, 5%, 15% and 20%), each treatment was given 3 repetitions, and observed mortality of larvae of Aedes Agypti after treated for 24 hours. The results showed that 1. There is an effect of pomegranate peel extract against Aedes aegypti larvae mortality for 24 hours, and at a level of 20% has the highest mortality rate (12.5 ± 2.43) 2. There is an effect of nut-grass extract extract against Aedes aegypti larvae mortality for 24 hours, and at a level of 20% has the highest mortality rate (6 ± 1.14) 3. The extract of pomegranate skin has the ability to kill the larvae of Aedes aegypti greater than the extract of nut-grass
EFFECT OF CHERRY LEAF (Muntinga calabora) BIOINSECTICIDES EXTRACT TOWARDS MORTALITY OF WORM SOIL (Agrotis ipsilon) AND ARMYWORM (Spodoptera exiqua) ON PLANT LEEK (Allium fistolum) Binawati, Diah Karunia; Amilah, Susie
WAHANA Vol 61 No 2 (2013)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v61i2.1267

Abstract

Leek plant (Allium fistulosum) that grown at Village Treceh Sajen Mojokerto District Pacetoften suffer from some loss that caused by the attacks from pests such as worm soil (Agrotisipsilon) and armyworm (Spodoptera exiqua). The use of synthetic insecticides is very dangerous,because the residue will affect the health of consumers. Cherry plant (Muntinga calabura) containsactive substances such as tannins, saponins and flavonoids that can be used as bioinsecticides.This study aims to determine whether there is an effect of cherry leaf bioinsecticidesextract towards mortality of worm soil and armyworm in a in vitro manner, and to observe thegrowth of leek plant at harvest scallion age 2 months. The study design used is a CompleteRandomized Design, with 4 treatments using cherry leaf extract (0%, 2.5%, 5% and 7.5%), andgiven 6 replications. Samples of worm soil and armyworm caterpillars each are 120 which itsmortality will be observed for 24 hours. Leek plant sample are 60 plants. The results of dataanalysis showed a 24-hour LC50 value for worm soil is 4.240% and the 24-hour LC50 values forarmyworm is 3,434%. These results indicate that the cherry leaf extract is more effective toannihilate armyworm than it is to annihilate worm soil.To observe the effect of cherry leaf bioinsecticides treatment extract towards the growth ofleek plant leaves were harvested 2 months of age. Results of test F (0.024) indicates that there is asignificant effect of cherry leaf extract towards the growth of leek plants. The results of the LSDanalysis of these data showed that the P3 treatment with a concentration of 7.5% is the one thatproduces the best growth of the plant.
DAYA HAMBAT EKSTRAK SAMBILOTO (ANDROGRAPHIS PANICULATA) DENGAN BERBAGAI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI SALMONELLA TYPHI Amilah, Susie; Prasetyo, Agus
WAHANA Vol 56 No 1 (2011)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/wahana.v56i1.1391

Abstract

Andrographis paniculata atau yang dikenal dengan nama sambiloto adalah tumbuhan yang biasa dipakai oleh masyarakat untuk mengobati typoid. Tujuan penelitian ini adalah untuk mengetahui daya hambat ekstrak sambiloto terhadap pertumbuhan Salmonella typhi dan mengetahui konsentrasi yang paling efektif dalam menghambat pertumbuhan bakteri Salmonella typhi. Bahan uji yang digunakan adalah biakan bakteri Salmonella typhi yang diisolasi dari penderita yang dirawat di RSU Dr. H. Koesnadi Bondowoso, yang kemudian diuji dengan menggunakan ekstrak sambiloto dengan berbagai konsentrasi. Adapun konsentrasi yang digunakan adalah konsentrasi 5%, 10%, 20%, 30%, dan 40%. Pengamatan jumlah koloni kuman dilakukan dengan metode Total Plate Count (TPC) . Data dianalisis menggunakan uji F yang dilanjutkan dengan LSD. Kesimpulan penelitian ini menunjukkan bahwa Andrographis paniculata atau sambiloto dengan berbagai konsentrasi memiliki kemampuan dalam menghambat pertumbuhan bakteri salmonella typhi. Konsentrasi yang memberikan daya hambat terbaik adalah konsentrasi ekstrak 40%. Dengan demikian masyarakat dapat menggunakan sambiloto sebagai obat alternatif dalam melakukan pengobatan terhadap penderita typoid.