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All Journal Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
M. F. Al-Habib
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Genetic Association and Expression of Myoglobin Gene Related to Mineral Content in IPB-D2 Chickens P. Liong; A. K. Alfiah; I. Khaerunnisa; S. Darwati; R. Afnan; A. B. Dharmayanthi; M. F. Al-Habib; C. Sumantri
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 12 No. 1 (2024): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.12.1.26-33

Abstract

Myoglobin (MB) gene encodes the protein of myoglobin, which is a protein found in muscle tissues that plays a crucial role in binding and storing oxygen. This study aimed to analyze the polymorphism of the MB gene, examine its relationship with the meat mineral content, and analyze its expression in the liver and thigh muscle tissue of IPB-D2 chicken. A total of 55 IPB-D2 chickens were used in this study. Identification of the gene polymorphism, quantification of the mineral content, and gene expression were performed using the direct sequencing method, the Atomic Absorption Spectroscopy (AAS) method, and the qRT-PCR, respectively. Chicken thigh muscle and liver tissues were used as the source of mRNA in this study. The statistical analysis methods used were the chi-square test to test Hardy-Weinberg equilibrium, and the T-test for mineral association analysis and gene expression analysis. IPB-D2 chicken on average contains 17.81, 0.22, 16.50, and 0.34 mg/kg of Fe, Se, Zn, and Mn, respectively. Two SNPs were found in 5’UTR of the MB gene, namely SNP g.17 G>T and SNP g.25 T>C. Genotype TT (g.17 G>T) and genotype CC (g.25 T>C) were significantly (P<0.05) associated with high Fe content in IPB-D2 chicken. The gene expression analysis showed that MB mRNA expression in the liver was not statistically different between high Fe (TT/CC) and low Fe (GG/TT and GT/TC) genotype combination. Meanwhile, in the thigh muscle, MB mRNA expression in TT/CC genotype combination was significantly higher than GG/TT and GT/TC genotype combination (P<0.05). These results suggest that the identified polymorphisms in the IPB-D2 MB gene could serve as references for investigating similar gene in other chicken breeds, especially regarding Fe, Se, Zn, and Mn mineral content.
Asosiasi c.248 A>G gen CD1B dengan IgY dan Titer ND pada Ayam Sentul Galur Sensi-1 Agrinak M. F. Al-Habib; S. Murtini; A. Gunawan; N. Ulupi; C. Sumantri
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 8 No. 1 (2020): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.8.1.30-35

Abstract

The aim of this study was to identify the polymorphism of the CD1B gene in c.248 in exon 3 and its association with IgY and ND titers in sentul chicken Sensi-1 Agrinak line. IgY is the main antibody of chickens while the ND titer is an ND specific antibody, both of which have an important role in the response of disease resistance. The Cluster of differentiation 1B (CD1B) gene is one of the major genes of disease resistance that functions in representing antigens through specific immune responses. This study used 17 sensi-1 chicken lines. The study was conducted by PCR sequencing method, ELISA test, and HI test. The result showed mutation on c248 A>G in exon 3. The SNP were polymorphic and Hardy Weinberg equilibrium. Thre were two A and G alleles and 3 genotypes namely AG, GG, and AA. The frequency of the GG genotype was higher (0.41) compared to other genotypes. The AG genotype were associated with IgY production and were not associated with ND titers.
Co-Authors A. B. Dharmayanthi A. GUNAWAN A. K. Alfiah C. Sumantri I. Khaerunnisa N. Ulupi P. Liong R. Afnan S. Darwati S. Murtini