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All Journal Buletin Peternakan Jurnal Ilmu Ternak Jurnal Ilmu Ternak Veteriner Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan

C. Sumantri

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Author-ID : 1376505

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary Other

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Hubungan Keragaman Gen Leptin dengan Kualitas Fisik Daging Sapi Lokal Di Ciamis (Relationship between Leptin Gene Diversity with Physical Quality of Local Beef In Ciamis) Nena Hilmia; R.R Noor; C. Sumantri; R. Priyanto; Gurnadi E
Jurnal Ilmu Ternak Vol 15, No 2 (2015)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v15i2.9528

Leptin terlibat dalam berbagai proses fisiologispembentukan lemak. Keragaman gen Leptin karena adanya SNP Arg25Cys pada exon 2, mempunyai hubungan dengan deposisi lemak, yang dapat mempengaruhi kualitas daging. Penelitian ini bertujuan untuk mengetahui keragaman gen leptin dan hubungannya dengan kualitas fisik daging sapi lokal di Ciamis Jawa Barat. Penelitian ini menggunakan 14 sampel daging bagian udamaru yang diperoleh dari peternakan rakyat.Sampel DNA diisolasi dari daging. Amplifikasi DNA menggunakan PCR, dan penentuan genotipe dianalisis dari hasil sequencing produk PCR. Hasil penelitian menunjukkan Gen Leptin pada sapi lokal Ciamis bersifat polimorfik, terdapat tiga alel, yaitu C, T (memiliki mutasi Arg25Cys) dan H (memiliki mutasi Arg25His) serta terdapat tiga genotipe CC, CT dan CH. Perbedaan genotipe gen Leptin tidak berpengaruh terhadap kualitas fisik daging sapi lokal di Ciamis.Kata kunci: Kualitas daging, Leptin

Polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) Gene at Locus (Pit-1-Hinf1) and its effects on dam body weight and milk production of local sheeps C. Sumantri; D. Herdiana; A. Farajallah; D. Rahmat
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Pituitary-Specific Transcription Factor-1 (Pit-1) is a transcription factor with critical role in the transcriptional regulation of multiple genes in the pituitary. The objective of this research was to identify polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) gene at Locus (Pit-1-Hinf1) and to investigate any possible associations of Pit-1 genotypes on dam body weight, milk production and milk quality in local sheep at the Jonggol Animal Science Teaching and Research Unit (JASTRU), Fact. Anim. Sci. Bogor. Agric. Univ. A total number of 161 blood samples were collected from 3 local sheep, namely Garut from Wanaraja (55 hd), Garut from Margawati (23 hd) and lactating ewes (83 hd) from JASTRU farm in Bogor. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products were digested with a Hinf1 enzyme restriction. Fragments of Pit-1gene at locus Pit-1-Hinf1 was detected by a silver-staining method. A length of 637 base pairs (bps) of the Pit-1 gene of local sheep was successfully amplified. The Hinf1 restriction enzyme cut the PCR product into three different length of fragments succesively at 345, 137, and 115 bps designated as A allele; whilst B allele had four fragments at 283, 137, 115, and 62 bps respectively. The locus of Pit-1-Hinf1 was polymorphic in local sheep from Jonggol, however it was monomorfic in Garut sheep. The frequencies of A and B alleles were 0,806 and 0,194 respectively. Pit-1 genotypes had no significant effect on dam body weight and milk production. This result is indicating that the use of single locus Pit-1-Hinf1 in Pit-1 gen is less effective to be used as a candidate in selecting dam body weight and milk production in these three local sheep. Key words: Local Sheep, Pit-1 Gene, Polymorphism, Dam, Milk

Polymorphism of growth hormone receptor (GHR) gene in Holstein Friesian dairy cattle Restu Misrianti; C. Sumantri; A. Anggraeni
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 4 (2011): DECEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Growth hormone gene have a critical role in the regulation of lactation, mammary gland development and growth process through its interaction with a specific receptor. Growth hormone (GH) is an anabolic hormone which is synthesized and secreted by somatotrop cell in pituitary anterior lobe, and interacts with a specific receptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested as candidate gene for traits related to milk production in Bovidae. The purpose of this study was to identify genetic polymorphism of the Growth Hormone Receptor (GHR) genes in Holstein Friesian (HF) cattle. Total of 353 blood samples were collected from five populations belonging to Cikole Dairy Cattle Breeding Station (BPPT-SP Cikole) (88 samples), Pasir Kemis (95 samples), Cilumber (98 samples), Cipelang Livestock Embryo Center (BET Cipelang) (40 samples), Singosari National Artificial Insemination Centre (BBIB Singosari) (32 samples) and 17 frozen semen samples from Lembang Artificial Insemination Center (BIB Lembang). Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques then PCR products were genotyped by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. There were two allele dan three genotypes were found namely: allele A and G, Genotype AA, AG and GG repectively. Allele A frequency (0.70-0.82) relatively higher than allele G frequency (0.18-0.30). Chi square test show that on group of BET Cipelang, BIB Lembang and BBIB Singosari population were not significantly different (0.00-0.93), while on group of BET Cipelang, BIB Lembang dan BBIB Singosari population were significantly different (6.02-11.13). Degree of observed heterozygosity (Ho) ranged from 0.13-0.42 and expected heterozygosity (He) ranged from 0.29-0.42. Key Words: Growth Hormone Receptor, Polymorphism, Holstein Friesian Cattle

Verification for the control of kappa-casein gene on milk protein yield of friesian-holstein in dairy central region of West Java A. Anggraeni; C. Sumantri; A. Farajallah; E. Andreas
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Genotipe of BB kappa kasein (ĸ-kasein) gene positively influences high milk protein yield. The aim of this research was to verify the use of the к-casein gene as marker of assisted selecion (MAS) of high protein yield in domestic Holstein-Friesian (HF) cattle. Genotyping blood samples was conducted for lactating cows from BPPT-SP Cikole (82 head) and a number of small holders in KPSBU Lembang (190 head). Genotyping the к-casein gene was also conducted for active and non active AI bulls (HF) from BIB Lembang (25 head) and BBIB Singosari (32 head). Varian genotipes were identified trough DNA migration, whereas frequencies of alleles were calculated by Nei Method (1987). The effects of variant genotypes on protein yield and other milk component yields were studied in lactating cows (56 head in BPPT-SP Cikole and 111 head in KPSBU Lembang) by applying Least Square Means (LSM) method of the General Linear Model (GLM). Genotyping the ĸ-kasein gene resulted in three genotypes, namely AA, AB and BB, with two alleles, namely A and B. It was identified that a very limited number of lactating cows with BB genotype (0-9%). These cows produced higher protein yield around 3.37-3.84% than that of AA cows, while AB cows produced protein yield in between, though these differences were not statistically significant (P>0,05). In contrast, AA cows tended to produced higher milk fat yield than BB cows. Cows BB and AB seemingly produced higher dry matter, while both specific density and pH were not affected by variant genotypes of the к-casein gene. The results showed that BB genotype of the к-casein gene tended to be quitely consistent in controlling high protein yield, so it would be a good oppurtinity to be used as MAS of milk protein yield in HF domestic. Key words: Protein Yield, Dairy Cattle (HF), к-Casein Gene, Genotype BB

Evaluation of genetic diversity of Katingan catlle and their genetic relationship with some other local catlle through DNA microsatellite analysis Bambang Ngaji Utomo; R.R. Noor; c. Sumantri; I Supriatna; E.D. Gurnadi
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 2 (2011): JUNE 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Katingan cattle is one of Indonesian animal genetic resources located in Katingan District, Central Kalimantan. The Katingan cattle is predicted to be extint, therefore it is necessary to conserve the cattle. Breed characterization is the primary step in any conservation program. Characterization was done using 10 microsatellite markers to evaluate the genetic diversity of Katingan Cattle in three subpopulations and their genetic relationship with some other local cattle. A total number of 72 random whole blood of Katingan samples consisted of Pendahara (20 samples), Buntut Bali (20 samples), and Tumbang Lahang (32 samples) were used. The samples of Bali, PO, and Limousine Cattle were 11, 6, and 3 respectivelly. The number of 136 aleles were found with the variation from 9 aleles (ILSTS089) to 18 aleles (ILSTS013) and the average of the alele was 13.6 aleles per locus. A number of alel from Tumbang Lahang was higher (10.8 aleles) than Pendahara (10.4 aleles) and Buntut Bali subpopulation (7.3 aleles). Some loci produced polymorphic diagnostic aleles which varied from 1-7 types of allele. HEL013 and BM1818 had four aleles, while ILSTS026 and ILSTS089 had five and six aleles respectively. ILSTS029 and ILSTS036 had seven aleles. The diagnostic aleles were also found in Tumbang Lahang subpopulastion, as well as in Pendahara, and Buntut Bali. Heterozigositas values of Pendahara, Buntut Bali, and Tumbang Lahang subpopulation were 0.454, 0.478, and 0.529 respectively. While the average of heterozigositas (Ĥ) was 0.492. Subpopulation of Tumbang Lahang was closer genetically to Pendahara (0.169) than Buntut Bali (0.173) and also the subpopulation was closer genetically to PO cattle (0.259) when compared to Buntut Bali (0.311) and Pendahara (0.329). The population of Katingan cattle was within one kluster with PO Cattle. Key Words: Katingan Cattle, Microsatellite, Genetic Diversity

Genetic distance estimation of local swamp buffaloes through morphology analysis approach. Anneke Anggraeni; C. Sumantri; L. Praharani; Dudi .; E. Andreas
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 3 (2011): SEPTEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Information about genetic distances among populations (geographics) of local swamp buffalo is less published. Genetic distance analysis was done among seven local swamp buffalo populations trhough a morphological analysis. Female and male buffaloes were observed for a total number of 905 hds, originating from the Provices of NAD (100 hds), North Sumatera (51 hds), Banten (180 hds), Central Java (203 hds), South Kalimantan (121 hds), NTB (200 hds), dan South Sulawesi (50 hds). Genetic variation among buffalo populations was calculated by discriminant function of Mahalonobis distance. Genetic distance was estimated by MEGA program. Morphometric measurements of female buffalo in North Sumatera were larger (P < 0,01) than those of buffaloes in Banten, South Kalimantan, NAD and South Sulawesi. Morphometric measurements of male buffalo in South Sulawesi were larger than those of buffaloes in Central Java, Banten, and NAD. Chest width was the highest different morphometric factor (0.969). Buffalo from NTB had the highest similarity (95.0%), followed by buffaloes from North Sumatera (74.51%) and South Sulawesi (74.19%); while the lowest one was from South Sulawesi (38.02%) and Banten (49.44%). Morphological distribution map indicated existence of three local swamp buffalo groups. Grup 1 was presented by buffalo from NTB, distributed in the II and III quadrants. Group 2 was presented by buffaloes from five locations (North Sumatera, Central Java, South Kalimantan, South Sulawesi, and Banten), distrributed in I and II quadrants, and a smaller part distributed in III and IV quadrants. Group 3 was presented by buffaloes from NAD and a smaller part from South Kalimantan and Banten, distributed in IV quadrant. The lowest genetic distance was identified between buffaloes in NAD and South Kalimantan (0.348), while the highest one was between buffloes in Banten and South Kalimantan (1.883). Based on phenogram tree structure, the observed local swamp buffaloes could be classified into 3 cluster, namely: Cluster 1 from South Kalimantan, NAD and Banten; Cluster 2 from South Sulawesi, Central Java and North Sumatera, and Cluster 3 from NTB. Key Words: Swamp Buffalo, Morphology, Discriminant Analysis, Genetic Distance

Karakteristik Morfometrik Ukuran Tubuh dan Bentuk Domba Ekor Gemuk Pulau Madura dan Rote dengan Menggunakan Analisis Komponen Utama A. Gunawan; C. Sumantri
Buletin Peternakan Vol 31, No 4 (2007): Buletin Peternakan Vol. 31 (4) November 2007
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v31i4.1236

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Physicochemical, Microbiological, and Organoleptic Characteristics of IPB D1 Braised (Ungkep) Chicken at Different Storage Temperature and Different Storage Time D. Nafisah; Z. Wulandari; A. Apriantini; C. Sumantri
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 12 No. 1 (2024): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.12.1.1-7

Braised (Ungkep) chicken is one of the varieties of processed IPB D1 chicken that can be produced to increase consumer acceptance. The aim of this research was to analyze the physicochemical, microbiological and organoleptic characteristics of IPB-D1 braised chicken at refrigerator and freezertemperatures with storage times of 0, 7, and 14 days. A factorial completely randomized design was used in this study. The pH value and Total Plate Count (TPC) were significantly influenced by storage time (P<0.05). The storage temperature factor and the interaction between temperature and storage time did not have a significant effect on all variables. The TPC value up to the 14th day was still below the maximum limit, therefore it was still safe to consume.

Egg Production and Quality of IPB D3 Chicken and It’s Repeatability Estimation M. F. Hawari; C. Sumantri; S. Darwati
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 12 No. 1 (2024): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.12.1.8-13

High egg production and good egg quality for IPB D3 chicken is very important to produce a lot and quality DOC, then a high repeatability value is also needed to maintain continuous DOC availability. The purpose of this study was to examine the estimated repeatability value of egg production and eggquality as well as the production performance and quality of chicken eggs IPB D3. The observed poultry were 30 IPB D3 G3 females and 90 eggs. Repeatability values were analyzed using a variance table and egg production and quality data were analyzed descriptively. The repeatability value of egg production is 0.42 (high), the Haugh unit repeatability of egg is 0.40 (medium), the egg weight repeatability value 0.67 (high), the egg yolk score repeatability value 0.40 (medium) and the egg index repeatability value 0.95 (high). Egg weight 40.76 g, Haugh unit value 76, and egg production 44.8%.

Weight Performance of 4th Generation IPB-D3 Local Chickens Aged 1-3 Months and It`s Heritability Value I. Galib; C. Sumantri; S. Darwati; S. Murtini
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 12 No. 1 (2024): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jipthp.12.1.14-20

Bogor Agricultural University local chicken which named IPB-D3 is a prospective male line obtained from the chicken selection program IPB-D1. The establishment of IPB-D3 chickens aims to produce local chickens with fast growth weight. This research aims to evaluate the performance of local chickens from IPB-D3 Generation 4 (G4) and its heritability values. The observed samples include 10 males and 30 females of IPB-D3 chickens of Generation 3 (G3) aged 21-40 weeks as parent and 100 Day Old Chickens (DOC) IPB-D3 G4 up to 12 weeks old. The results obtained that IPB-D3 G4 chickens show an average body weight for males 1021.44 g and females around 648.33 g at the age of 12 weeks. The feed given to IPB-D3 G4 chickens is the same as the parents, namely it has nutrients below commercial feed because the feed given uses a mixture of local feed which are bran and corn which already used in Sinar Harapan Farm Sukabumi. Weight gain is the change in body weight that occurs over a specific period, and the weight gain of IPB-D3 G4 chickens is relatively fast, especially from the 2nd to the 3rd month, with a weight gain rate reaching 406.27 g. Growth rate from IPB-D3 G4 chickens experiences a slowdown at the age of 2 months and falls below their optimal growth rate. Another aspect to report is the mortality rateof IPB-D3 G4 chickens, which is quite high at the DOC and female age due to environmental conditions and disease attacks. However, the mortality rate decreases with the increasing age of the chickens, and no deaths are found in 12 weeks old male chickens. The heritability value of body weight in IPB-D3 G4 chickens is high at the age of 4 weeks and can be used as a basis for selection based on body weight.

Co-Authors A. Anang A. Anggraeni A. Apriantini A. B. Dharmayanthi A. GUNAWAN A. K. Alfiah A. S. Harahap A. U. Khairiyah ACHMAD FARAJALLAH Anneke Anggraeni Asep Gunawan Bambang Ngaji Utomo D. Herdiana D. Nafisah D. Rahmat Dudi . E. Andreas E.D. Gurnadi F. Amri G. S. Afifah Gurnadi E I Supriatna I. Galib I. Khaerunnisa Jakaria Jakaria L. Cyrilla L. Praharani M. A. Pagala M. A. Rizqi M. Akramullah M. F. Al-Habib M. F. Hawari N. N. Miraj N. Ulupi Nena Hilmia P. Liong R. Afnan R. Priyanto R.R Noor R.R. Noor Restu Misrianti S. Darwati S. Murtini Sumiati Z. Wulandari

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