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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Ners
Pelupessy, Ninny Meutia
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Nursing

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Diagnostic Value of Typhidot and TUBEX-TF in Pediatric Typhoid Fever Using Composite Reference Standard Salipadang, Eunike Jaequeline; Rauf, Syarifuddin; Pelupessy, Ninny Meutia; Rahimi, Rahmawaty; HK, Adhariana; Sarmila, Besse
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 31 No. 2 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v31i2.2368

Abstract

Typhoid fever, a systemic infection remains a significant global health concern, especially in endemic areas. Typhidot and TUBEX-TF present a quick, easy, and cost-effective, however, their diagnostic performance still needs to be evaluated. This study aims to evaluate Typhidot and TUBEX-TF diagnostic accuracy for typhoid fever in children using CRS composed of blood culture and PCR and recording its performance on fever days. This was a diagnostic study with a cross-sectional design involving children suspected of typhoid fever aged 1 to 18 years, in 7 hospitals in South Sulawesi, Indonesia from November 2023 to April 2024. Venous blood samples were collected from each respondent and tested using TUBEX-TF, Typhidot, and composite reference standard, a combination of blood culture and nested PCR, to improve diagnostic accuracy. Of 85 samples, 27 (31.8%) were positive for Typhidot, 14 (16.4%) for TUBEX-TF +4, and 44 (51.7%) for TUBEX-TF >+6. The positive rate for Typhidot dan TUBEX-TF >+6, was highest in the first week of fever (p<0.01). The sensitivity, specificity, PPV, and NPV (95% CI) of Typhidot were 43.55% (31.0-56.7), 100% (85.2-100), 100%, and 39.66%, respectively, and TUBEX-TF >+6 were 70.97% (58.1-81.8), 73.91% (51.6-89.8), 88%, and 48.57%, respectively. The combination of Typhidot and TUBEX-TF was also tested and resulted in higher diagnostic values (p=0.000). Therefore, combined Typhidot and TUBEX-TF testing is proposed as an effective strategy in the diagnosis of typhoid fever.
Divisi Infeksi dan Penyakit Tropis Infeksi Measles Pada Anak Perempuan 3 Tahun Rifani, Muhammad; Pelupessy, Ninny Meutia; Artati, Ratna Dewi; Baso, Aidah Juliaty A.
Jurnal Ners Vol. 8 No. 1 (2024): APRIL 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v8i1.20190

Abstract

Measles is a highly contagious infection that spreading especially in populations that are dense with exhibit low immunity. The best protection is active immunization during childhood. Without vaccination, measles morbidity and mortality is very high. Here, we report a case of a 3-year-old child presenting with fever, malaise, productive cough, runny nose, conjunctivitis with Koplik's spot and maculopapular rash. Suspected measles infection is confirmed by positive measles virus IgM antibody and reverse transcriptase polymerase chain reaction in blood and urine. The patients are isolated and treated symptomatically. Management is given to these patients includes educating the patient's parents about the patient's illness and complications of the disease and the provision of medical form of infusion RL 16 ml / hour, Paracetamol 150 mg / hour, Ambroxol 7 mg / 8 hours, and Vitamin A 200.000 IU for 2 days. Guidelines for measles immunization in infants given one at the age of 9 months given the measles antibody toddlers whose mothers had decreased at the age of 9 months. Methode : This case study was researched only in the form of a single unit, but was analyzed in depth covering a fairly wide range of aspects. Conclution : In young patients, complete recovery was achieved within two weeks. In patients with fever and maculopapular rash and fatigue, measles should be considered even in all children patients. Early diagnosis, followed by isolation and patient registration are important measures to prevent local outbreaks.
Co-Authors Artati, Ratna Dewi Baso, Aidah Juliaty A. HK, Adhariana Muhammad Rifani, Muhammad Rahimi, Rahmawaty Salipadang, Eunike Jaequeline Sarmila, Besse Syarifuddin Rauf