Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Jurnal Bios Logos BIOMA : Jurnal Biologi Makassar Caraka Tani: Journal of Sustainable Agriculture Biosaintifika: Journal of Biology & Biology Education
Setiaji, Arkan
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
INDUCTION AND GROWTH KINETICS CALLUS OF TOMATO (SOLANUM LYCOPERSICUM) Setiaji, Arkan; Annisa, RR Rifka; Rumiyati, Rumiyati; Semiarti, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21704

Abstract

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ?Permata?) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.
Pemanfaatan Kriopreservasi untuk Penyimpanan Bibit Anggrek Jangka Panjang Setiaji, Arkan; Annisa, Rr Rifka
JURNAL BIOS LOGOS Vol 10, No 2 (2020): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.11.2.2020.28793

Abstract

ABSTRAKTanaman anggrek (Orchidaceae) telah menjadi komoditas penting di pasar perdagangan internasional karena keeksotisan dan daya tahan bunganya. Meskipun keragaman anggrek spesies menduduki peringkat kedua setelah Asteraceae dan hibridanya menduduki peringkat pertama, namun anggrek menghadapi ancaman kepunahan dan penurunan kualitas genetik. Program pemuliaan, seperti persilangan dan penyisipan gen unggul, serta perbanyakan in vitro perlu didukung dengan cara memastikan ketersediaan eksplan. Dalam rangka penyimpanan untuk kebutuhan jangka panjang, eksplan dapat disimpan menggunakan teknik kriopreservasi. Review ini bertujuan untuk memberikan gambaran umum terhadap kemajuan penelitian di bidang kriopreservasi, khususnya tanaman anggrek. Melalui program penyimpanan jangka panjang, gen-gen dan eksplan unggulan baik untuk pengembangan anggrek hibrida atau khususnya untuk tujuan konservasi anggrek langka bisa dipastikan ketersediaannya. Dalam artikel review ini telah dibahas berbagai riset tentang teknik kriopreservasi pada biji, protokorm, dan serbuk sari anggrek. Kemajuan pengembangan teknik telah dicapai, namun masih diperlukan banyak modifikasi agar suatu protokol kriopreservasi dapat diterapkan pada semua spesies dan hibrida anggrek.Kata kunci: kriopreservasi; anggrek; suhu dingin; eksplan ABSTRACTOrchid plants (Orchidaceae) have become important commodities in the international trades market because of their exoticism and flower endurance. Although the diversity of orchid species is ranked second after Asteraceae and number of hybrids are ranked first, but orchids face the threat of extinction and genetic degradation. Breeding programs, including such as crossing and insertion of superior genes, and in vitro propagation need to be supported by ensuring the availability of explants. In the context of maintaining storage for long-term needs, explants can be stored using cryopreservation techniques. This review aims to provide an overview of the progress of research in the field of cryopreservation, specifically orchid plants. Through a long-term storage program, superior genes and explants both for the development of hybrid orchids or in particular for the purpose of conservation of rare orchids can be ensured availability. In this review article various researches on cryopreservation techniques in seeds, protocorms, and pollen have been discussed in this article. Advances in technical development have been achieved, but many modifications are still needed so that a cryopreservation protocol can be applied to all orchid species and hybrids.Keywords: cryopreservation; orchids; cold temperatures; explants
OPTIMASI DOSIS PEMUPUKAN UREA UNTUK PERTUMBUHAN VEGETATIF CABAI BESAR (Capsicum annuum L.) DALAM POT Ardani, Windarti; Setiaji, Arkan
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 11 No. 1 (2026): Bioma : Januari - Juni 2026
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v11i1.47750

Abstract

Plants cultivated in pots face limitations in access to nutrients and water, as well as other growth-supporting factors, compared to those planted in the field. Therefore, understanding the plant's response to the potted cultivation model warrants attention. This study aimed to determine the optimal dose of Urea fertilizer that supports the vegetative growth of large chili (Capsicum annuum). Large chili plants of the Panex F1 variety were sown for 28 days and then transplanted into treatment pots arranged in a Randomized Complete Block Design with 4 replications. The treatments consisted of varying urea doses: 0, 1, 3, 5, 7, and 9× the reference dose of 388.5 mg, equivalent to 0 – 3.497 g urea per plant. This fertilizer dose was split into 5 applications via watering over a 40-day growth period. The variables observed were plant height, number of leaves, and shoot dry weight. Analysis of variance results indicated that urea dose variations did not significantly affect plant height and number of leaves but had a highly significant effect on the shoot dry weight variable (p<0.01). Through quadratic regression model analysis (R2=0.305), the optimal urea fertilizer dose for large chili pepper was determined to be 3 grams per plant, with a predicted shoot dry weight of 2.42 grams. Keywords: Kata kunci : large chili pepper, pot experiment, urea nitrogen, vegetative phase                                               
Potential Role of Plant Growth-Promoting Halotolerant Bacteria in Enhancing Shallot Growth under Salinity Stress Setiaji, Arkan; Sulastri, Sulastri; Sopandie, Didy
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 3 (2025): July
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i3.98106

Abstract

Soil salinization, driven by seawater intrusion, significantly challenges agricultural productivity in coastal regions. Horticultural crops, such as shallots, are especially sensitive to salinity stress, which impairs growth, nutrient uptake, and bulb yield. This study explored halotolerant plant growth-promoting bacteria from saline soils in East Nusa Tenggara, Indonesia, to reduce salinity stress in shallots. Seventeen bacterial isolates were screened for halotolerance, and eight of them were capable of growing at 1,250 mM NaCl (OD600 ≥ 0.5). Selected halotolerant isolates also exhibited the ability to produce indole-3-acetic acid (IAA) and exopolysaccharides (EPS), solubilize P, K, and Zn, produce siderophores, and exhibit 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity with varying tolerance at salinity levels up to 1,000 mM NaCl. Inoculation with these isolates significantly improved shallot seedling growth under 90 to 230 mM NaCl, with Enterobacter hormaechei demonstrating the best performance. Bacterial inoculation elevated 47 to 64% proline and 15 to 107% NO3− levels in shallot leaves compared to uninoculated plants, contributing to osmotic adjustment and enhanced nutrient assimilation under salt stress in laboratory trials. Single-strain (E. hormaechei) and a consortium of compatible strains (E. hormaechei strain R11 and M119.1, Klebsiella pneumoniae strain A95, K. variicola strain R198, and Pseudochrobactrum asaccharolyticum strain C167.1) inoculation significantly increased shoot dry weight (100% and 69% each) compared to uninoculated plants under salt stress. These findings advance the current understanding of microbial-assisted salinity mitigation and support broader strategies for climate-resilient, sustainable agriculture in saline-prone coastal regions.
Co-Authors Annisa, RR Rifka Ardani, Windarti Didy Sopandie Endang Semiarti Rumiyati, Rumiyati Sulastri Sulastri