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All Journal Jurnal Sinergitas PkM & CSR Nusantara Science and Technology Proceedings Indonesian Journal of Life Sciences
Putu Virgina Partha Devanthi
Indonesia International Institute For Life Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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LATIHAN MANAJEMEN PENGOLAHAN SAMPAH BAGI MASYARAKAT KELURAHAN CEMPAKA PUTIH TIMUR, JAKARTA [WASTE MANAGEMENT TRAINING FOR CEMPAKA PUTIH TIMUR VILLAGE, JAKARTA] Katherine Kho; Solmaz Aslanzadeh; Siti Muslimatun; Putu Virgina Partha Devanthi
Jurnal Sinergitas PKM & CSR Vol 4, No 1 (2019): October
Publisher : Universitas Pelita Harapan

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Abstract

DKI Jakarta is currently in a state of waste emergency because Bantar Gebang landfill is estimated to reach its full capacity by 2021. This problem should not be solely solved by the city’s administrator but must also involve all citizens. As one of the efforts to contribute to the solution, training was given to representatives from RW 1 – 4 in Cempaka Putih Timur village. These representatives consist of community groups (RW) leaders, neighborhood groups (RT) leaders, and environmentalist. The goal of the training was to educate the representatives on how to manage waste properly, starting from reducing waste until the method of handling waste before it is disposed of. The final goal is to apply the content of the training in the village and reduce the amount of waste delivered to Bantar Gebang landfill. The approach used in the training was student – centered learning to improve the participants’ understanding. The activity was started with a discussion, followed by sharing of waste management method, followed by games for deeper understanding. The analysis of the result of the training, analyzed by the difference in pre – game and post game’s score and survey to the trainees, indicates an increased understanding of the participants.
Development of Selective Media for Komagataeibacter intermedius and Dekkera bruxellensis from a Mixed Culture Ferren Pratama; Putu Virgina Partha Devanthi; Katherine Kho
Nusantara Science and Technology Proceedings Join Proceeding "Basic and Applied Science Conference (BASC) 2021 & 1st Education Research and Appli
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1106

Abstract

Komagataeibacter intermedius and Dekkera bruxellensis are some of the most commonly found bacteria and yeast, respectively, during Kombucha fermentation. The development of selective media for separating both species is essential to make an accurate cell enumeration. This study aimed to develop a selective media for the enumeration of K. intermedius and D. bruxellensis from a mixed culture sample. Hestrin and Schramm (HS) agar and Potato Dextrose Agar (PDA) were chosen as the enumeration media for K. intermedius and D. bruxellensis, respectively. To select for K. intermedius, HS agar was added with various concentrations of cycloheximide (0, 1, and 10 mg/mL), NaCl (2% and 5%), and acetic acid (1%). To select for D. bruxellensis, PDA was added with various concentrations of chloramphenicol (0, 0.3, and 3 ?g/mL) and NaCl (2% and 5%). Each culture (~106 log CFU/mL) was serially diluted and plated on the respective agar media, followed by incubation at 30°C for 1-6 days. The results showed that PDA containing 2% NaCl could completely suppress K. intermedius growth while permitting complete recovery of D. bruxellensis. However, it took 6 days until visible growth was observed by D. bruxellensis. Moreover, HS with the addition of 1% acetic acid successfully inhibited D.bruxellensis while allowing the complete recovery of K. intermedius. The findings suggested that the two media were suitable for separating K. intermedius and D. bruxellensis on agar media, thus allowing a more accurate cell counting in studies involving the mixed cultures of both species.
A Review on Bacterial Cellulose: Properties, Applications, Fermentative Production, and Molasses Potential as Alternative Medium Angela, Clara; Devanthi, Putu Virgina Partha
Indonesian Journal of Life Sciences 2021: IJLS Vol 03 No .01
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.106 KB) | DOI: 10.54250/ijls.v3i1.124

Abstract

Bacterial cellulose (BC) is an extracellular homopolymer produced by certain species of bacteria. It has demonstrated the potential as an alternative to plant cellulose with more appealing features such as unique nanostructure, high porosity, high crystallization index, high tensile strength, high water holding capacity, and a high degree of polymerization. These attributes facilitate BC utilization in various applications, ranging from the food industry, cosmetics, pharmaceutical, medical field, waste treatment, textile, and paper industry. Considering the advantages and wide range of applications, it is necessary to explore and improve the current industrial production to achieve a higher yield at a lower cost. This review article summarizes the BC properties and characteristics as well as its application in different fields. Furthermore, the potential of agricultural waste molasses for low-cost BC production is also discussed.
Co-Authors Angela, Clara Ferren Pratama Katherine Kho Siti Muslimatun Solmaz Aslanzadeh