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Asri, Mahanani Tri
Jurusan Biologi Universitas Negeri Surabaya
Agriculture, Biological Sciences & Forestry Education

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PATOGENITAS SPODOPTERA LITURA MULTIPLE NUCLEAR POLYHEDROSIS VIRUS (SPLTMNPV) YANG DILINDUNGI EKSTRAK KENCUR (KAEMPFERIA GALANGA) TERHADAP LAMA HIDUP LARVA SPODOPTERA LITURA Ainun, Irma Nur; Asri, Mahanani Tri; Rahayu, Yuni Sri
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Spodoptera litura is pest which causes leaf damage and distrub the process of photosynthesis that could result in loss of yields. This research aimed to describe the effect of adding the Kampferia galanga extract on SpltMNPV and to certain the effective concentration of Kampferia galanga extract as chemical protective material SpltMNPV of sun exposure to the length of life of S. litura larvae. The research design used was completely randomized design (CRD) with 5 treatments and 5 replications. The treatment was SpltMNPV+kaolin 1:4 which added with various concentrations of the Kampferia galanga extract 0%, 5%, 10%, 15%, and 20%. Each unit experiments used 10 S. litura larvaes. Data obtained in the form length of life of S. litura larvae were analyzed descriptively. The results showed that the treatment of SpltMNPV+kaolin 1:4 with various concentrations of Kampferia galanga extract (0%, 5%, 10%, 15%, and 20%) affected the length of life S. litura at 6 days, 6 days, 6 days, 5 days, and 5 days respectively with percentage of S. litura survive larvae at 28%, 22%, 12%, 12%, and 10% respectively. Treatment SpltMNPV + 1:4 kaolin + 15% Kampferia galanga extract was the most effective treatment in lenght of life of S. litura larvae.
PATOGENITAS SPODOPTERA LITURA MULTIPLE NUCLEOPOLYHEDROSIS VIRUS (SPLTMNPV) DENGAN BAHAN PEMBAWA TEPUNG BENGKUANG YANG TERPAPAR SINAR MATAHARI TERHADAP LAMA HIDUP LARVA SPODOPTERA LITURA Machfiroh, Aprilianti Nindya; Asri, Mahanani Tri; Rahayu, Yuni Sri
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Spodoptera litura Multiple Nucleopolyhedrosis Virus (SpltMNPV) is one of insects pathogens that infect armyworm so they can be as a natural armyworm enemies. The SpltMNPV are sensitive against the sun so that could reduce its pathogenicity level. This research aimed to determine the effective concentration of bengkuang flour to protect SpltMNPV against the sun exposure to maintain the length of life against armyworms. This research used a completely randomized design (CRD). The treatment consisted of one factors, namely bengkuang flour concentration with 4 treatments. The treatments were SpltMNPV + kaolin 1:4 added by bengkuang flour concentrations 0%, 1%, 2.5%, and 5% respectively. The result showed that SpltMNPV + kaolin 1:4 added by bengkuang flour concentrations of 0%, 1%, 2.5%, and 5% affected the length of armyworm life in 7 day, 7 day, 6 day, and 5 day respectively with percentage of armyworms 25%, 25%, 20%, and 5% respectively. The 5% concentration of bengkuang flour was the effective concentration to protect of SpltMNPV against the sun exposure.
MEDIA ALTERNATIF UNTUK PERTUMBUHAN SEL MIDGUT SPODOPTERA LITURA Aljauhari, Muhammad Ma?ruf; Asri, Mahanani Tri; Trimulyono, Guntur
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Cell culture is process when viable cell propagated in vitro in the medium. Midgut cell culture of S. litura is very useful as hosts for SpltMNPV virus that can control S. litura. This study aimed to determine the increase in the number of S. litura midgut cells and long incubation time of S. litura midgut cells in RPMI 1640, DMEM, and mixture of RPMI 1640 medium and DMEM as alternative medium with initial inoculum 4.0 x 105 cell/ml. This research used Completely Randomized Design (CRD) with 4 treatments and 3 repetitions so that there are 12 treatment unit. Data was analyzed using ANOVA (Analysis of Variance). Further tests are LSD (Least Significant Difference). The results showed that the highest increase of the number of S. litura midgut cells was obtained in Grace's medium (control) with 23125 times inoculum, the mixture of RPMI 1640 and DMEM medium was  21950 times inoculum, RPMI 1640 medium was  11725 times, DMEM medium was  10400 times inoculum. Spodoptera litura midgut cells form a monolayer in a 24-hour incubation in Grace's medium, the mixture of RPMI 1640 and DMEM medium, and DMEM, whereas the monolayer formation in RPMI 1640 medium is in a 48-hour incubation. The result showed that the mixture of RPMI 1640 and DMEM medium is an .optimum alternative medium that support the growth of Spodoptera litura midgut cell.
Co-Authors Ainun, Irma Nur Aljauhari, Muhammad Ma?ruf Machfiroh, Aprilianti Nindya Trimulyono, Guntur YUNI SRI RAHAYU