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Trimulyono, Guntur
Jurusan Biologi Universitas Negeri Surabaya
Agriculture, Biological Sciences & Forestry Education

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MEDIA ALTERNATIF UNTUK PERTUMBUHAN SEL MIDGUT SPODOPTERA LITURA Aljauhari, Muhammad Ma?ruf; Asri, Mahanani Tri; Trimulyono, Guntur
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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Abstract

Cell culture is process when viable cell propagated in vitro in the medium. Midgut cell culture of S. litura is very useful as hosts for SpltMNPV virus that can control S. litura. This study aimed to determine the increase in the number of S. litura midgut cells and long incubation time of S. litura midgut cells in RPMI 1640, DMEM, and mixture of RPMI 1640 medium and DMEM as alternative medium with initial inoculum 4.0 x 105 cell/ml. This research used Completely Randomized Design (CRD) with 4 treatments and 3 repetitions so that there are 12 treatment unit. Data was analyzed using ANOVA (Analysis of Variance). Further tests are LSD (Least Significant Difference). The results showed that the highest increase of the number of S. litura midgut cells was obtained in Grace's medium (control) with 23125 times inoculum, the mixture of RPMI 1640 and DMEM medium was  21950 times inoculum, RPMI 1640 medium was  11725 times, DMEM medium was  10400 times inoculum. Spodoptera litura midgut cells form a monolayer in a 24-hour incubation in Grace's medium, the mixture of RPMI 1640 and DMEM medium, and DMEM, whereas the monolayer formation in RPMI 1640 medium is in a 48-hour incubation. The result showed that the mixture of RPMI 1640 and DMEM medium is an .optimum alternative medium that support the growth of Spodoptera litura midgut cell.
PENGGUNAAN EKSTRAK DAUN SRIKAYA (ANNONA SQUAMOSA) SEBAGAI PENGENDALI JAMUR FUSARIUM OXYSPORUM SECARA IN VITRO Purwita, Ayu Anggun; Indah, Novita Kartika; Trimulyono, Guntur
LenteraBio: Berkala Ilmiah Biologi Vol 2, No 2 (2013)
Publisher : Universitas Negeri Surabaya

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This study aimed to determine the phytochemical profile of srikaya extract qualitatively and the antifungal activities srikaya leaves extract to inhibition on the growth of Fusarium oxysporum and find out the optimal concentration of srikaya leaves extract on inhibiting the F. oxysporum growth. The study were designed by randomized completely design with one factor treatments with 5 concentration.Antifungal activity test to be used solid dilution method. Concentration include 0% (control), 5%, 5.5%, 6%, and 6.5%. Each treatment was repeated with four replications. Observation was conducted after incubation for 7 days at room temperature. The parameters observed was inhibition F. oxysporum growth with indicated size of the diameter mycelial colony. The results showed that the srikaya leaves extract had an inhibitory effect on mycelial colony A. flavus growth. Optimum concentration in this study was 6.5% concentration because it can inhibit the growth of F. oxysporum with an average of 2,84cm ± 0,30 dimater colonies and the percentage inhibition is 60%.
Co-Authors Aljauhari, Muhammad Ma?ruf Asri, Mahanani Tri Novita Kartika Indah Purwita, Ayu Anggun