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OPTIMASI DAN VALIDASI METODE KLT-DENSITOMETRI FASE TERBALIK UNTUK ANALISIS OKSIBENZON DALAM LOSION TABIR SURYA Andayani, Regina; Andira, Retno Permata; Armin, Fithriani
Indonesian Journal of Pharmaceutical Science and Technology Vol 11, No 3 (2024)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v11i3.43555

Abstract

 AbstractSunscreen is useful for protecting the skin against sun exposure.  Oxybenzone is one of the active ingredients in sunscreen which can cause skin allergies, redness, dryness, and acne. This study aims to optimize and validate the reverse phase densitometry thin layer chromatography (TLC) method for the analysis of oxybenzone in sunscreen lotions. The TLC silica gel 60 RP-18 F254 was used as the stationary phase, the mobile phase was acetonitrile: aquabidest (18:3). The Rf values of oxybenzone in lotion A and B were 0.63. The resolution of oxybenzone in lotions A and B were 2.53 and 3.2, respectively. The correlation coefficient (r) of oxybenzone was 0.999 in the range of 50 – 250 μg/mL, the limit of detection was 16.37 μg/mL and the limit of quantitation was 49.39 μg/mL. The precision of lotions A and B was ≤ 2%. The % recovery of oxybenzone in lotion A and lotion B was  99.33-99.63%. The results showed that the levels of oxybenzone lotion A and B were 3.4% and 2.59% successively.  The reverse phase TLC-Densitometry method is a valid method for the analysis of oxybenzone in sunscreen lotions.Keywords: Oxybenzone, sunscreen, validation, thin layer chromatography-densitometry, reversed-phase. OPTIMASI DAN VALIDASI METODE KLT-DENSITOMETRI FASE TERBALIK UNTUK ANALISIS OKSIBENZON DALAM LOSION TABIR SURYA Abstrak Tabir surya berguna untuk melindungi kulit dari paparan sinar matahari. Oxybenzone merupakan salah satu bahan aktif tabir surya yang dapat menyebabkan alergi kulit, kemerahan, kering dan berjerawat. Penelitian ini bertujuan untuk mengoptimalkan dan memvalidasi metode kromatografi lapis tipis (KLT) densitometri fase terbalik untuk analisis oksibenzon dalam lotion tabir surya. Silika gel TLC 60 RP-18 F254 digunakan sebagai fase diam, fase geraknya adalah asetonitril: akuabides (18:3). Nilai Rf oxybenzone pada lotion A dan B adalah 0,63. Resolusi oxybenzone pada lotion A dan B berturut-turut adalah 2,53 dan 3,2. Koefisien korelasi (r) oxybenzone adalah 0,999 dalam kisaran 50 – 250 μg/mL, batas deteksi adalah 16,37 μg/mL dan batas kuantitasi adalah 49,39 μg/mL. Nilai presisis lotion A dan lotion B adalah ≤ 2%. Persentase perolehan kembali oxybenzone pada lotion A dan lotion B adalah 99,33-99,63%. Hasil penelitian menunjukkan bahwa kadar oxybenzone lotion A dan B berturut-turut adalah 3,4% dan 2,59%. Metode TLC-Densitometry fase terbalik adalah metode yang valid untuk analisis oxybenzone dalam lotion tabir surya.Kata kunci: Oxybenzone, tabir surya, validasi, kromatografi lapis tipis-densitometri, fase terbalik.
Analisis Rendang Daging Sapi dan Daging Babi Hutan Menggunakan Metode Spektroskopi FTIR Kombinasi Kemometrik untuk Autentikasi Halal Andayani, Regina; Kesumaningrum, Ditya; Nisa, Tadzkia; Husni, Elidahanum; Suryati, Suryati; Syofyan, Syofyan; Dachriyanus, Dachriyanus
JSFK (Jurnal Sains Farmasi & Klinis) Vol 10 No 1 (2023): J Sains Farm Klin 10(1), April 2023
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.10.1.78-88.2023

Abstract

ABSTRAKRendang merupakan salah satu makanan tradisional Sumatra Barat dari olahan daging sapi. Harga daging sapi yang mahal, mendorong pemalsuan menggunakan daging babi hutan. Penelitian ini bertujuan untuk mengembangkan metode analisis autentikasi rendang sapi menggunakan spektroskopi FTIR kombinasi kemometrik. Sampel yang digunakan adalah campuran daging sapi dan babi hutan yang dibuat dalam 11 konsentrasi (training data set) serta rendang rumah makan (testing data set). Komponen lemak rendang diekstraksi menggunakan metode Folch dan Bligh Dyer kemudian dianalisis dengan spektroskopi FTIR. Spektra FTIR yang dihasilkan digunakan sebagai variabel pemodelan kemometrik. Untuk kuantifikasi, model PLS di bilangan gelombang 1250-950 cm-1 memberikan model terbaik pada metode Folch dengan nilai R2 kalibrasi 0,9946, R2 prediksi 0,9954, RMSEC 0,0328,  RMSEP 0,0402 dan pada metode Bligh Dyer, model PCR di bilangan gelombang 1800-500 cm-1 memberikan model terbaik dengan nilai R2 kalibrasi 0,9940, R2 prediksi 0,9919, RMSEC 0,0345, RMSEP 0,0457. Untuk klasifikasi, model DA di daerah gabungan bilangan gelombang 1800-1600 cm-1 dan 1250-950 cm-1 dengan metode Folch dan bilangan gelombang 1800-650 cm-1 dengan metode Bligh Dyer, berhasil mengelompokkan training data set menjadi beberapa kelas dan mengelompokkan testing data set ke dalam kelas sapi. Dengan demikian disimpulkan bahwa analisis spektroskopi FTIR kombinasi kemometrik merupakan metode screening yang cepat, sederhana dan murah untuk autentikasi rendang sapi dan babi hutan.Kata kunci: autentikasi, rendang sapi, babi hutan, FTIR, kemometrik ABSTRACTRendang is one of West Sumatra's traditional foods made from processed beef. The high price of beef encourages counterfeiting using wild boar meat. This study aims to develop a method of authentication analysis of beef rendang using FTIR spectroscopy in combination with chemometrics. The sample used is a mixture of beef and boar meat made in 11 concentrations (training data set) and restaurant rendang (testing data set). The rendang fat component was extracted using the Folch and Bligh Dyer methods and then analyzed by FTIR spectroscopy. The resulting FTIR spectra are used as a chemometric modelling variable. For quantification, the PLS model at wave number 1250-950 cm-1 gives the best model in the Folch method with a calibration R2 value of 0.9946, prediction R2 of 0.9954, RMSEC 0.0328, RMSEP 0.0402 and in the Bligh Dyer method, the model PCR at wavenumber 1800-500 cm-1 gave the best model with a calibration R2 value of 0.9940, prediction R2 of 0.9919, RMSEC 0.0345, RMSEP 0.0457. For classification, the DA model in the combined area of wave numbers 1800-1600 cm-1 and 1250-950 cm-1 with the Folch method and wave numbers 1800-650 cm-1 with the Bligh Dyer method, successfully grouped the training data set into several classes and grouped testing data set into cow class. Thus it was concluded that FTIR spectroscopic analysis combined with chemometrics is a fast, simple, and inexpensive screening method for the authentication of beef rendang and wild boar.Keywords: authentication, beef rendang, wild boar, FTIR, chemometrics 
Analisis Kadar Vitamin B1 dalam Kacang Panjang (Vigna sinensis L.) Menggunakan Spektrofotometri Ultraviolet-Visible Armin, Fithriani; Andayani, Regina; Hilson, Denny; Azzaky, Azzaky
Jurnal Geografi, Lingkungan dan Kesehatan Vol 1 No 1 (2023): Jurnal Geografi, Lingkungan dan Kesehatan
Publisher : Program Studi Pendidikan Geografi Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jglk.1.1.9977

Abstract

A Research of vitamin B1 in long beans (Vigna sinensis L.) analyzed using ultraviolet-visible (uv-vis) spectrophotometry has been carried out at a wavelength of 435.6 nm. Vitamin B1 reacted with bromthymol blue to form a yellow ion-associated complex in an ammonia-ammonium chloride as a buffer solution pH 7.6 which could be observed with a polyvinyl alcohol solubilizing agent. Long beans were boiled in water for 5, 10 and 15 minutes at 100oC. Vitamin B1 in long beans (Vigna sinensis L.) without boiling 0.0981 % ± 0.00021, boiling 5, 10 and 15 minutes were 0.0935 % ± 0.00015; 0.0834 % ± 0.00010 and 0.0743 % ± 0.00010 and the vitamin B1 in the long bean boiled water at 5, 10 and 15 minutes boiling were 0.00102 % ± 0.000004; 0.00110 % and 0.00117 % ± 0.00001. It’s can be concluded that boiling long beans (Vigna sinensis L.) can cause a decrease in vitamin B1 which decreases with increasing boiling time and vitamin B1 in the cooking water increase due to the nature of vitamin B1 which is easily soluble in water