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All Journal AL KAUNIYAH Journal of Biotechnology and Natural Science DIMASLIA

Putri, Diah Asta

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Author-ID : 1454101

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Methanolic Extracts of Waru Leaves (Hibiscus tiliaceus L.) for Controlling Armyworms (Spodoptera litura F.) Putri, Diah Asta; Saputra, Arib Cahyo
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021): June
Publisher : Universitas Ahmad Dahlan

Botanical extracts have been reported as biopesticides, which act as antifeedants, insect growth regulators and repellents. As biopesticides, botanical extracts offer a more sustainable solution to pest control as chemical pesticides have a residual problem and are prone to pest resistance. The present study was conducted to evaluate the efficacy of waru leaves extract to control S. litura larvae in vitro conditions. The extract was prepared by maceration with methanol as a solvent. Third instar larvae of S. litura were randomly grouped into 6 treatments consisting of 0%, 3%, 6%, 9%, 12% and positive control (Lannate). Each treatment consisted of 3 replications. Leaf Dipping method was used in the treatment. Observations were made for up to 4 days. The parameters observed were changes in larval morphology, mortality, LC50 and LT50. Data were analyzed using One Way ANOVA (followed by LSD) and Probit analysis. The results showed that the highest activity of waru leaf extract was found at a concentration of 12%, which caused a mortality of 53% larvae. The LC50 value was at a concentration of 11.72%, and the LT50 value was 3.287. It can be concluded that the leaf extract has the potential to control S. litura larvae.

Ethanolic Extracts of Shallot Leaves (Allium ascalonicum L.) as Botanical Pesticide for Controlling Fall Armyworms (Spodoptera fugiperda J.E. Smith) Putri, Diah Asta; Nur Aini, Pradipta
Journal of Biotechnology and Natural Science Vol. 1 No. 2 (2021): December
Publisher : Universitas Ahmad Dahlan

The application of botanical pesticide is a positive long term approach to control S. frugiperda. Shallot leaves contain acetogenin compound, hence it has the potential to become botanical pesticide. This study is aimed at finding out the effects of ethanolic extract of shallot leaves on S. frugiperda larvae in vitro. Extract is prepared using maceration method with ethanol solvent. The method of treatment is Leaf Dipping. The study applies Completely Randomized Design with concentration of shallot leaf extract as treatment at 0 ppm, 250 ppm, 500 ppm, 750 ppm dan 1000 ppm. Each treatment consists of four repetitions. Examination is conducted for 24 hours. Examination parameter is mortality of larvae and LC50. Data are analyzed with One Way ANOVA (proceeded with LSD) and Probit analysis. The results suggest that shallot leaf extract at the concentration of 1000 ppm appears to be the most effective on the mortality of larvae. The obtained LC50 value is 263,02 ppm. The study indicates that shallot leaf extract can be applied as an alternative synthetic insecticide to control ini S. frugiperda.

Vinca alkaloids effects on the morphological characters of Canavalia ensiformis Putri, Diah Asta; Rahyuni, Mita; Sarwati, Mega
Journal of Biotechnology and Natural Science Vol. 2 No. 1 (2022): June
Publisher : Universitas Ahmad Dahlan

Productivity and quality of crops can be increased through polyploidy plants induced by antimitotic compounds such as vinka alkaloids.The use of vinka alkaloids has never been applied to Sword bean (Canavalia ensiformis), a tropical legume belonging to the Fabaceae family. This study aimed to determine the effects of vinka alkaloids treatment on the morphological characters of Sword bean. The treatment consist of four different vinka alkaloids concentrations (0.5%, 1.00%, 1.50%, 2.0%, and untreated (0.0%) act as control. The plants were arranged in a completely randomized design. Treated plants were characterized based on morphological traits such as plant height, leaf area and dry seed weight. The results obtained revealed significant difference (p≤0.05) in the morphological traits of the treated plants when compared with the control. Overall, 2.0% vinka alkaloids concentration resulted in higher average plant height by 193.8 ± 1.86, leaf area increments by 88.6 ± 5.1 (left leaf), 115.1 ± 4.7 (middle leaf), 86.2 ± 3.8 (right leaf) and the dry seed weight by 1.7 ± 3.6. The improvement of the traits is concentration dependent and increases with increment in vinka alkaloids concentration. Thus, we suggested that 2.0% concentration should be employed in improving Sword bean growth and yield.

Morphological Variation of “Jamur So” (Scleroderma sp.) from Purworejo Regency, Central Java Pratiwi, Ambar; Putri, Diah Asta; Gultom, Putri Syifa; Indah, Rosiana; Sasongko, Hadi
Journal of Biotechnology and Natural Science Vol. 2 No. 1 (2022): June
Publisher : Universitas Ahmad Dahlan

Fungal diversity is high in Indonesia, one of which is Scleroderma sp. Scleroderma forms a symbiotic relationship with Gnetum gnemon. Its fruiting body is puff-ball shaped, and its gleba can be consumed because of its taste and nutritious. Study on the Scleroderma’s diversity in Purworejo Regency has not been done. In this study, morphological characteristics were used as main identification character. Scleroderma samples were collected from Grabag, Kemiri, and Kaligesing and then observed their morphology and spore characteristics. Morphological traits observed on fruit body shape (globose and kidney-shaped); and ornamentation on peridium layer (cracked, nodule and peeling). Chemical content, temperature, and humidity of the soil were also observed as supporting data.

Co-Authors Gultom, Putri Syifa Hadi Sasongko, Hadi Indah, Rosiana Nur Aini, Pradipta Oktira Roka Aji Pratiwi, Ambar Rahyuni, Mita Saputra, Arib Cahyo Sarwati, Mega Suwartiningsih, Nurul

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