Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal CORD
Anitha Karun
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Library & Information Science

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
PHOTOSYNTHETIC ACCLIMATIZATION IN ZYGOTIC EMBRYO CULTURED PLANTLETS OF COCONUT (COCOS NUCIFERA L.) S. Naresh Kumar; V. Rajagopal; Anitha Karun
International Coconut Community Journal Vol 17 No 02 (2001): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v17i02.352

Abstract

It is important to understand the physiological acclimatization process in order to provide the favorable growing conditions, to increase the survival rate and to produce vigorous seedlings for field planting for better establishment. These aspects assume significance because the embryo culture technique is used for germplasm collection in coconut. The zygotic embryos from eight month old selfed nuts from West Coast Tall (WCT) cultivar of coconut were collected for embryo culture. The field planted seedlings produced by embryo culture and those raised from nursery were compared for photosynthetic activity under field grown conditions. Photosynthetic and water relation parameters were studied in the embryo cultured plantlets at different stages of acclimatization under green net house conditions. The photosynthetic rate increased gradually during acclimation with maximum rate atfour months after transfer to pots (under 50% shade; stage 4). The embryo cultured plantlets had higher photosynthetic rates compared to the nursery raised plants under field conditions. Transpiration rate increased with stage during acclimation. Embryo cultured plants had low transpiration rate as compared to the nursery raised plants. Consequently embrvo cultured plants had higher WUE (A/E). The WUE increased during acclimation. The Fv/Fm ratio increased gradually from 0.69 to 0.72 till stage 4. The field‑grown embryo culture plants had higher Fv/Fm compared to the nursery raised plant. This increase in Fv/Fm during acclimation indicated the increase in photochemical activity and photosystem II electron transport efficiency. The study indicated increase in photosystem efficiency and carbon assimilation efficiency of embryo cultured plantlets of coconut during acclimatization.
DESIGN AND ANALYSIS OF COCONUT EMBRYO CULTURE EXPERIMENTS Anitha Karun; K. Muralidharan; K. K. Sajini; V.A. Parthasarathy
International Coconut Community Journal Vol 19 No 01 (2003): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v19i01.366

Abstract

The experimental design and data analysis procedures followed for standardizing the CPCRI protocol of coconut zygotic embryo culture are described. The availability of human resources (for initiating the cultures as well as periodic sub-culturing), laboratory space for maintaining culture, infrastructure for preparation of adequate culture media etc, are the important factors to be considered at the planning stage. Since the response of interest per experimental unit is discrete (germinated or not), it is desired to have a “plot size” of 20. Based on the experimental errors obtained for various treatments, attempts were made to obtain the replication size for a specified value of treatment difference to be tested. In most of the experiments, per cent germination was not following the assumption of ANOVA , even after transformation. In such situation, the chi-square test was employed to study the influence of treatments on germination. The coefficient of variation in most of the experiments was below 20 %.
Cryopreservation of mature coconut embryos by desiccation method Anitha Karun; K.K. Sajini; V.A. Parthasarathy
International Coconut Community Journal Vol 21 No 1 (2005): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v21i01.395

Abstract

Mature embryos of West Coast Tall variety of coconut could be cryopreserved after desiccation pretreatments and retrieved into plantlets. The desiccation pretreatment and optimum duration for dehydration of zygotic embryos were standardized. The maximum retrieval of healthy plantlets was obtained from the embryos subjected to 18 hours silica gel or 24 hours laminar airflow desiccation pretreatment. Irreversible damage caused by desiccation to the death of the shoot meristem was noticed when the water content reduced to 20%.
Co-Authors K. K. Sajini K. Muralidharan K.K. Sajini S. Naresh Kumar V. Rajagopal V.A. Parthasarathy