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All Journal El-Hayah : Jurnal Biologi Biofaal Journal
Lathif, Yudrik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Berlian, SOJ A3 and Jawa Local Rice Varieties Phylogenetically Distinct From Oryza sativa Indica and Oryza sativa Japonica Lathif, Yudrik; Retnaningtyas, Riri Wiyanti; Listyorini, Dwi; Suharti, Suharti
El-Hayah : Jurnal Biologi Vol 6, No 4 (2018): EL-HAYAH (VOL 6, NO 4,March 2018)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i4.5883

Abstract

The genetic resources identification of Indonesian local rice varieties is a crucial work should be done to conserve our native germplasm. This research aimed to know the taxonomical position of East Java local rice varieties including Jawa (JW), Berlian (BR), and SOJ A3 (SJ) using DNA barcode based on rbcL gene. Total DNA of each sample was isolated from leaves. A pair of forward 5'-ATG TCA CCA CAA ACA SJA AC-3' and reverse 5'-TCG GTA CCT GCA GTA GC-3' primers were used to amplify fragments of rbcL gene resulting in 751bp, 755bp, and 754bp fragments from BR, SJ, and JW varieties, respectively. Phylogenetic tree reconstruction revealed that our three local varieties were forming a cluster separated from the widely cultivated subspecies Oryza sativa Indica and Oryza sativa Japonica. However, further studies are necessary to reveal a more precise position of the local varieties in a phylogenetic tree on the species level.
BIOAKTIF ALAMI DARI TAPAK DARA (Catharanthus roseus) TERHADAP PENYAKIT HIPERTENSI DENGAN PENDEKATAN DASAR PENELITIAN IN SILICO Taihuttu, Monalisa Pertiwi Jeriska; Watung, Fernando A; Lathif, Yudrik
Biofaal Journal Vol 5 No 2 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i2pp116-122

Abstract

Ajmalicine is a natural secondary metabolite compound found in the Catharanthus roseus plant, which has potential as an antihypertensive agent. Docking simulations were used to explore the molecular interactions between Ajmalicine and its identified target protein, the Alpha-2A adrenergic receptor, using three servers: PharmMapper, SwissTargetPrediction, and SuperPred. The analysis was supported by software such as PyMOL and LigPlus, Discovery Studio 2016 Client, and PyRx 0.8. The results showed that Ajmalicine interacts with the target protein, and this interaction was compared with a control compound, Clonidine, a chemical already used as an antihypertensive drug. The findings revealed that Ajmalicine binds to the same site on the target protein as Clonidine.
Co-Authors Dwi Listyorini Fernando Andre Watung Jayawarsa, A.A. Ketut Riri Wiyanti Retnaningtyas Taihuttu, Monalisa Pertiwi Jeriska