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All Journal Jurnal Hortikultura Indonesia (JHI) JURNAL BIOLOGI INDONESIA Annales Bogorienses
Leksonowati, Aryani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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IRADIASI SINAR ? PADA BIAK TUNAS KENTANG HITAM (SOLANOSTEMON ROTUNDIFOLIUS) EFEKTIF UNTUK MENGHASILKAN MUTAN Witjaksono, Witjaksono; Leksonowati, Aryani
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3075

Abstract

Irradiation of ??ray at shoot culture of Kentang hitam (Solanostemon rotundifolius) is effective for mutant production. Kentang hitam is sterile and vegetatively propagated andtherefore its genetic diversity is narrow. Mutation is an alternative way to increase genetic heterogeneity. Irradiation of shoot cultures with different doses followed by culturing of the inoculum (leaf, petiole and internodes) from that irradiated culture on a regeneration medium MS containing 5 mg/l BA and 0.1 mg/l NAA has resulted in curvilinear respon of growth, morphogenesis and plant regeneration. The growth variables increase from 0 to 6 Gy and thendecrease to 25 and plateu or increase a little at 35 Gy and growth death wes observed at 50 Gy. Leaf and petiole inocula were more responsive than the internode. Respon of growth of shootregeneration of 50% were obtained at doses of 10-12.5 Gy. However higher level of doses, such as 25 Gy had also been effective for inducing mutant. Morphological and growth different were observed from growth in tissue culture to the field. Mutants were recovered, forexample, the one with early flowering.Keywords : Solanostemon rotundifolius, Irradiation, gammarays, cultur in vitro, mutan
Growth Response of Moringa oleifera Lam. Shoot Culture to Benzyladenine and Nitrogen Modification Handayani, Tri; Leksonowati, Aryani; Riastiwi, Indira; Ridwan; Witjaksono
Jurnal Hortikultura Indonesia (JHI) Vol. 12 No. 1 (2021): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.12.1.59-68

Abstract

Pada perbanyakan tanaman secara in vitro, sitokinin dan hara nitrogen merupakan beberapa komponen utama yang mempengaruhi pertumbuhan dan morfogenesis tanaman. Pada penelitian ini, komponen utama tersebut diuji untuk optimasi pertumbuhan kultur tunas kelor dengan perlakuan berikut: (1) konsentrasi BA yaitu 0.00, 0.05, 0.10, 0.25, 0.50, 1.00 mg L-1, (2) total konsentrasi N dalam medium yakni 20, 40, 60, 80 mM; (3) perlakuan nisbah NO3-:NH4+ yakni 0:1, 1:3, 1:1, 3:1, 1:0. Rancangan yang digunakan adalah acak lengkap dengan 10 ulangan. Hasil penelitian menunjukkan bahwa perlakuan BA 0.25 mg L-1 menghasilkan proliferasi tunas terbaik dengan jumlah buku tertinggi (8.07±3.48). Respon pertumbuhan tunas terhadap perbedaan konsentrasi nitrogen cenderung bersifat parabolik dengan konsentrasi optimum 40–60 mM pada medium standar MS. Perlakuan nisbah nitrat/ amonium menunjukkan pertumbuhan dan biomassa tunas in vitro yang optimal pada nisbah NO3-:NH4+ 3:1. Medium MS dengan modifikasi total konsentrasi nitrogen 40-60 mM dengan nisbah NO3-:NH4+ 3:1 dapat digunakan untuk proliferasi tunas kelor secara in vitro untuk tujuan perbanyakan klonal dari genotipe terpilih. Kata kunci: Benzyladenin, in vitro, kelor, nisbah nitrat/ amonium, total N
Tissue Culture Propagation of “Hausa potato” (Solenostemon rotundifolius (Poir) JK Morton) Witjaksono, Witjaksono; Leksonowati, Aryani
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
Publisher : BRIN

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Abstract

We have developed an effective plant propagation method of „hausa potato‟ through shoot proliferation in vitro, acclimatization in the greenhouse and transplantation of the plantlet on the individual media container for planting material production. The shoot cultures were initiated from shoots that grew from tuber collected from the field, disinfested using standard method and transferred to MS medium with 1 mg/l of BA for shoot proliferation. The proliferating shoots were then subcultured to MS medium with no growth regulators to induce rooting and enlarge the shoot. The plantlets were acclimatized in a plastic box containing medium of a mixture of cocopeat, sands, rice husk charcoal and top soil covered with plastic film to maintain humidity. The plantlets survived at 85% and almost 100% grew into planting materials ready for transplanting in the soil. The planting materials survived in the field and produced tuber normally. 
Co-Authors Riastiwi, Indira Ridwan Tri Handayani Witjaksono Witjaksono Witjaksono