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All Journal JURNAL BIOLOGI INDONESIA Jurnal Kefarmasian Indonesia
Kusumawati, Dinihari Indah
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Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology

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THE GENUS CHITINOPHAGA ISOLATED FROM WANGGAMETI NATIONAL PARK AND THEIR LYTIC ACTIVITIES Meliah, Siti; Kusumawati, Dinihari Indah; Lisdiyanti, Puspita
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3734

Abstract

 ABSTRACTThe utilization of bacterial enzymes in commercial industry, agriculture, waste treatment and health is preferred over other sources like plants and animals sources because they provide many advantages for different applications. The genus Chitinophaga which was first described as chitinolytic Myxobacteria, known as chitin destroyer or chitin eater due to their capability to hydrolyze chitin. The present study aims to isolate, characterize, identify, and assay the indigenous bacteria from Wanggameti National Park for their lytic activity againts chitin, cellulose and protein as an initial step in bio-prospecting of Sumba Island. Eleven yellow pigmented isolates were obtained from soil and decayed wood samples using ST21 and Water Agar media. They formed halo on VY/2CX medium. Physiological charazterization showed that two isolates were able to produce catalase but none of them produced urease. The phylogenetic analysis based on 16S rRNA gene sequences indicated that all isolates belong to the genus Chitinophaga that consisting of Chitinophaga filiformis, Chitinophaga ginsengisoli, Chitinophaga pinensis, and Chitinophaga sancti. They were deposited in InaCC under the name InaCC B1254 to InaCC B1264. Qualitative analysis of their lytic activity exhibited that all strains were able to lyse chitin and cellulose. The strains with the highest chitinase and cellulase activity are InaCC B1260 and InaCC B1258 strains, respectively, both of them are C. pinensis. Hereafter, C. filiformis showed the highest proteolytic activity in skim milk casein amongs all strains at 1.14±0.08. Keywords: Chitinophaga, chitinase, cellulase, protease, Sumba 
Keragaman Bakteri Endofit Penghasil L-Asparaginase Bebas L-Glutaminase sulistiyani, tri ratna; kusumawati, dinihari indah
Jurnal Kefarmasian Indonesia VOLUME 9, NOMOR 1, FEBRUARI 2019
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v9i1.1302

Abstract

Endophytes are potential as a source of active compound producer. Endophytes that is isolated from tropical medicinal plants has been getting attention due to its high biodiversity and active compound producing ability. L-asparaginase is the first enzyme used as chemotherapeutic agent for leukemia. The aim of this study is to collect the endophytic bacteria associated with tropical medicinal plants from Sumba Island and investigate the activity of L-asparaginase without L-glutaminase from endophytic bacteria isolates. The samples Gliricidia sepium, Pittosporum moluccanum, Clerodendrum buchanani, and Zingiberaceae are collected from Wanggameti, East Sumba, NTT. Samples are sterilized using surface-sterilization method and endophytic bacteria are isolated using plant piece method on R2A media. Selected endophytic bacteria are identified by 16S rDNA sequences. L-asparaginase screening is conducted using modified R2A with addition of L-asparagine and phenol red as colour indicator. A total of 34 isolates of endophytic bacteria were collected from 5 samples. A total of 14 genus consisted of 17 different bacterial species were obtained from 34 selected isolates. Endophytic bacteria of P. stutzeri strains of SMKL1 and R. radiobacter strains of SMKW2 from the Kahili plant were needed as L-glutaminase-free L-asparaginase and were potential to be candidate of leukemia cancer chemotherapy
Co-Authors Meliah, Siti PUSPITA LISDIYANTI TRI RATNA SULISTIYANI