Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Makara Journal of Science Jurnal Kefarmasian Indonesia
TRI RATNA SULISTIYANI
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jalan Raya Bogor-Jakarta Km. 46, Cibinong 16911 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
BAKTERI ENDOFIT YANG DIISOLASI DARI AKAR Eurycoma longifolia DAN POTENSINYA SEBAGAI PENGENDALI JAMUR PATOGEN TANAMAN Sulistiyani, Tri Ratna; Meliah, Siti; Damayanti, .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6303.007 KB) | DOI: 10.29122/jbbi.v7i1.3997

Abstract

Eurycoma longifolia (pasak bumi) is known as a medicinal plant that contains biologically active compounds. Studies on endophytic bacteria associated with pasak bumi and their biocontrol activities have not been widely reported. The objective of this study is to isolate potential endophytic bacteria associated with E. longifolia possessing biocontrol activity against plant pathogenic fungi, Sclerotium rolfsii, Fusarium solani, F. oxysporum, and Colletotrichum gloeosporioides. Endophytic bacteria were isolated from the roots of E. longifolia using the plant piece method and identified based on 16S rRNA genes analysis. Antagonist test of bacterial isolates was conducted by dual confrontation method. The mechanisms of fungal growth inhibition were evaluated based on their ability to produce hydrolytic enzymes, antibiotic, and volatile organic compounds. Two isolates were obtained and identified as Stenotrophomonas maltophilia (Apb1) and Serratia marcescens (Apb2). Apb2 was able to inhibit the growth of four tested fungi and showed protease, chitinase as well as cellulase activities. The crude extract and volatile organic compound produced by Apb2 were active against F. solani growth.Keywords: biocontrol, endophytic, Eurycoma longifolia, fungi, inhibition mechanism ABSTRAKEurycoma longifolia (pasak bumi) dikenal sebagai tanaman obat yang mengandung beberapa senyawa aktif secara biologis. Penelitian mengenai bakteri endofit yang berasosiasi dengan tanaman pasak bumi berikut aktivitas biokontrolnya belum banyak dilaporkan. Penelitian ini bertujuan mengisolasi bakteri endofit potensial dari tanaman E. longifolia yang memiliki aktivitas biokontrol terhadap empat strain uji jamur patogen tanaman, yaitu Sclerotium rolfsii, Fusarium solani, F. oxysporum, dan Colletotrichum gloeosporioides. Bakteri endofit diisolasi dari akar E. longifola menggunakan metode plant piece dan diidentifikasi berdasarkan analisis gen 16S rRNA. Uji antagonis isolat bakteri dilakukan dengan metode konfrontasi ganda. Mekanisme penghambatan jamur patogen tanaman dievaluasi berdasarkan kemampuannya dalam memproduksi enzim hidrolisis, senyawa antibiotik, dan senyawa organik volatil. Dua isolat bakteri endofit berhasil diperoleh dan teridentifikasi sebagai Stenotrophomonas maltophilia  (Apb1) dan Serratia marcescens (Apb2). Isolat Apb2 mampu menghambat pertumbuhan keempat jamur yang diuji dan menunjukkan aktivitas protease, kitinase dan selulase. Ekstrak kasar dan senyawa organik volatil yang dihasilkan oleh isolat Apb2 aktif menghambat pertumbuhan F. solani.
DIVERSITY OF FRUIT FLIES (DIPTERA: TEPHRITIDAE) ATTRACTED BY ME LURE IN CSC-BG GERMPLASM CARAMBOLA PLANTATION Riastiwi, Indira; Paradisa, Yashanti Berlinda; Mambrasar, Yasper Michael; Raunsai, Marlin Megalestin; Perwitasari, Urip; Volkandari, Slamet Diah; Sari, Nurul Fitri; Sulistiyani, Tri Ratna; Ibo, Leberina Kristina
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221151-157

Abstract

Diversity of fruit flies (Diptera: tephritidae) attracted by me lure in CSG-BG germplasm carambolae plantation. Germplasm Garden (KPN) Cibinong Science Center-Botanical Garden (CSC-BG) is one of the gardens that conserve and utilizes Indonesian local germplasm, one of which is carambolae. The current problem in KPN is the attack of fruit flies (Bactrocera spp.). This study was conducted to determine the diversity of fruit flies in the KPN carambola plantation area. A total of eight traps were placed randomly in the plantation area. Each trap contained 1 mL of methyl eugenol pheromone solution. Identification of fruit flies was carried out based on three morphological differences, they were wings, abdomen, and hind legs. There were 317 fruit flies collected and separated into three species, namely Bactrocera carambolae, B. papayae, dan B. umbrosa, of which B. carambolae had a dominance index of 0.74 and abundance index of 85%. Data on the types of fruit flies can be used as primary data to determine the preparation of pest prevention efforts.
Purification of Biotransformation Products of Cis-Isoflavan-4-ol by Biphenyl Dioxygenase of Pseudomonas pseudoalcaligenes KF707 Strain Expressed in Escherichia coli Sulistiyani, Tri Ratna
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Isoflavone has multiple beneficial effects on human health, especially through its antioxidant and anticancer activities. The biotransformation of isoflavone using byphenyl dioxygenase could be performed to extend the diversity of flavonoids and to improve their biological and physiological properties. Biotransformation of two enantiomers (3R, 4R)-cis-isoflavan-4-ol and (3S, 4S)-cis-isoflavan-4-ol by E. coli JM109 (pJHF108) carrying a biphenyl dioxygenase gene from P. pseudoalcaligenes KF707 produced two products, designated as CM1 and CM2. The products had a retention time of 11.9 and 14.6 min, respectively, and the same absorption peaks at 204, 220, and 275 nm. CM1 and CM2 had [M-H2O+H]+ at m/z 225. Based on the molecular mass and hydrolysis products, we proposed that epoxidation occurred on cis-isoflavan-4-ol. Chloroform extraction instead of ethyl acetate extraction was performed to improve the stability of cis metabolites, CM1 and CM2.
Keragaman Bakteri Endofit Penghasil L-Asparaginase Bebas L-Glutaminase sulistiyani, tri ratna; kusumawati, dinihari indah
Jurnal Kefarmasian Indonesia VOLUME 9, NOMOR 1, FEBRUARI 2019
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v9i1.1302

Abstract

Endophytes are potential as a source of active compound producer. Endophytes that is isolated from tropical medicinal plants has been getting attention due to its high biodiversity and active compound producing ability. L-asparaginase is the first enzyme used as chemotherapeutic agent for leukemia. The aim of this study is to collect the endophytic bacteria associated with tropical medicinal plants from Sumba Island and investigate the activity of L-asparaginase without L-glutaminase from endophytic bacteria isolates. The samples Gliricidia sepium, Pittosporum moluccanum, Clerodendrum buchanani, and Zingiberaceae are collected from Wanggameti, East Sumba, NTT. Samples are sterilized using surface-sterilization method and endophytic bacteria are isolated using plant piece method on R2A media. Selected endophytic bacteria are identified by 16S rDNA sequences. L-asparaginase screening is conducted using modified R2A with addition of L-asparagine and phenol red as colour indicator. A total of 34 isolates of endophytic bacteria were collected from 5 samples. A total of 14 genus consisted of 17 different bacterial species were obtained from 34 selected isolates. Endophytic bacteria of P. stutzeri strains of SMKL1 and R. radiobacter strains of SMKW2 from the Kahili plant were needed as L-glutaminase-free L-asparaginase and were potential to be candidate of leukemia cancer chemotherapy
Co-Authors Damayanti, . Ibo, Leberina Kristina Kusumawati, Dinihari Indah Meliah, Siti Paradisa, Yashanti Berlinda Raunsai, Marlin Megalestin Riastiwi, Indira Sari, Nurul Fitri Slamet Diah Volkandari, Slamet Diah Urip Perwitasari, Urip Yasper Michael Mambrasar, Yasper Michael