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Journal : Jurnal Midpro

Effect of White Oyster Mushroom (Pleurotus ostreatus) Ethanol Extract on Bacillus subtilis Bacteria Handrianto, Prasetyo; Ratnasari, Evy
Jurnal MIDPRO Vol 13 No 2 (2021): JURNAL MIDPRO
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/md.v13i2.342

Abstract

The increasing number of infectious diseases caused by bacteria, fungi, viruses and parasites in Indonesia, and one of the bacteria that causes this disease is Bacillus subtilis. Therefore, the role of antibacterial is very important for the treatment of this disease. One of the antibacterials from nature is the white oyster mushroom (Pleurotus ostreatus). The purpose of this study was to determine the effect of various concentrations of white oyster mushroom (Pleurotus ostreatus) extract which was effective as an antibacterial against Bacillus subtilis. The research method uses the paper disc diffusion method. The results showed that the bacterial inhibition zone at a concentration of 20 g/ml obtained an average of 9.5 mm in the medium category, a concentration of 40 g/ml obtained an average of 11.3 mm in the strong category, a concentration of 60 g/ml obtained an average of 13, 3 mm in the strong category, a concentration of 80 g/ml obtained an average of 15.5 mm in the strong category and a concentration of 100 g/ml obtained an average of 17.3 mm in the strong category. The conclusion of this research is white oyster mushroom can be used as an anti-bacterial for Bacillus subtilis.
Activity Of Lingzhi Fungan Ethanol Extract (Ganoderma Lucidum) Using Soxhlet Method For Bacteria Propionibacterium Acnes Handrianto, Prasetyo
Jurnal Midpro Vol. 12 No. 1 (2020): JURNAL MIDPRO
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/md.v12i1.220

Abstract

The purpose of this study is to know Lingzhi Mushroom extract (Ganoderma Lucidum) has antimicrobial activity against Propionibacterium acnes using Ethanol Solvent, the method used is the disc diffusion method using 6 repetitions, the concentration used is 20 µg / ml, 40 µg / ml, 60 µg / ml / ml, 80 µg / ml and 100 µg / ml, Lingzhi mushroom was extracted using ethanol solvent with the Soxhlet method. The results of the research data showed that the largest inhibitory zone was shown at a concentration of 100 µg / ml of 6.91 mm with a medium inhibitory category. The conclusion of this research is the Lingzhi mushroom is able to inhibit the activity of Propionibacterium acnes in the medium category.