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Mukhsia, Andi
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Micropropagation of Tetramolopium klossii (Moore) Mattfeld, Endemic Subalpine and Alpine Plant Species to New Guinea Ermayanti, Tri Muji; Hafiizh, Erwin Al; Martin, Andri Fadillah; Mandessy, Ary; Hermono, Arief; Mukhsia, Andi
Annales Bogorienses Vol. 15 No. 2 (2011): Annales Bogorienses
Publisher : BRIN

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The protocol of micropropagation of Tetramolopium klossii (Moore) Mattfeld through adventitious shoot proliferation was established. This is the first report of T. klossii tissue culture. Seeds were surface-sterilized by dipping them in 10% of ethanol for 1 min followed by soaking in 1% of sodium hypochlorite for 10 min. The sterile seeds were germinated on MS medium solidified with 8 g/l of agar, supplemented with 20 g/l of sucrose. Two-month old seedlings formed some shoot clumps on MS solid medium without addition of any plant growth regulators. Small clumps containing 4-6 adventitious shoots were used for shoot proliferation on MS solid medium containing 0; 0.1; 0.5; 1.0 and 2.0 mg/l of BAP (benzylaminopurine) or kinetin. The results showed that medium MS without cytokinins (control treatment), and the addition of 0.1 or 0.5 mg/l of BAP was more effective for shoot proliferation compared to the medium with addition of BAP at higher concentration and the addition of kinetin. In further subcultures, multiple shoots grown on MS medium without cytokinins was more vigorous than that of shoots grown on other media, so that the shoots were ready for rooting. Medium containing 0.5 mg/l of BAP was the best for maintaining multiple shoot formation. Shoots formed roots on MS medium containing half strength of macro nutrients.
In vitro Seed Germination and Shoot Multiplication of Seven Endemic Subalpine and Alpine Plant Species Grown on Mount Jaya, Papua, Indonesia Ermayanti, Tri Muji; Hafiizh, Erwin Al; Mandessy, Ary; Setyadi, Gesang; Mukhsia, Andi
Annales Bogorienses Vol. 18 No. 1 (2014): Annales Bogorienses
Publisher : BRIN

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Exploitation on plant population may put the endemic plants into an endangered state, hence, these plants will need to be conserved. In order to pursue conservation on endemic plants, we conducted in vitro seed germination and shoot multiplication of seven alpine and sub-alpine species endemic to Mount (Mt.) Jaya, in Papua, Indonesia, i.e. Tetramolopium klossii, Deschampsia klossii, Papuacalia cartenszensis, Epilobium hooglandii, Gaultheria novoguinensis, Rhododendron correoides, and Rhododendron culminicolum. These species are categorized as slow-growth plants found in higher altitude (over 3700 m above sea level) and low temperature of Mt. Jaya. Seeds were surface-sterilized using Na-hypochloride and germinated aseptically on Murashige and Skoog (MS) medium. Dytikinin benzyl adenine (BA) was used for shoot multiplication. Seedling cultures were maintained in a controlled environment with continuous low light intensity (800 lux) and at temperature 26-27oC. Results showed that most species had more than 80% of germination rate on MS medium after a week in culture. BA was required to enhance shoots multiplication. Woody Plant (WP) (Lloyd & McCown, 1981) medium gave better shoot multiplication for R. culminicolum.
Co-Authors Hafiizh, Erwin Al Hermono, Arief Mandessy, Ary Martin, Andri Fadillah Setyadi, Gesang Tri Muji Ermayanti