Pantouw, Carla Frieda
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Pengaruh peningkatan suhu pada fase pembentukan umbi tanaman kentang (Solanum tuberosum) cv. Granola Pantouw, Carla Frieda; Hapsari, Betalini Widhi; Hastilestari, Bernadetta Rina
Jurnal AGRO Vol 9, No 1 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/18117

Abstract

Kentang (Solanum tuberosum) merupakan salah satu bahan makanan yang penting di dunia. Budidaya komoditas ini umumnya berada di dataran tinggi dengan suhu yang rendah. Jumlah lahan pertanian di dataran tinggi semakin kecil disebabkan antara lain karena alih fungsi lahan. Penanaman kentang di dataran yang lebih rendah menjadi kendala karena adanya peningkatan suhu. Penelitian ini dirancang untuk mengetahui pengaruh ketinggian tempat dan perubahan suhu terhadap tanaman kentang pada fase pembentukan umbi. Tanaman kontrol ditanam pada ketinggian 2921 meter diatas permukaan laut (m dpl) dengan suhu siang/malam (190C/120C). Setelah fase pembentukan umbi, sebagian tanaman dipindah ke daerah dengan ketinggian 115 m dpl dengan suhu siang/malam (300C/240C). Perubahan ketinggian tempat dengan suhu yang berbeda mengakibatkan shade avoidance, perubahan akumulasi biomasa pada batang tanaman dan penurunan hasil panen. Hal ini disebabkan karena penurunan hasil fotosintesa, sukrosa, serta kadar klorofil yang disebabkan oleh faktor genetik dan metabolisme enzim. Oleh karena untuk mendukung permintaan komoditas kentang yang semakin meningkat, pemuliaan tanaman kentang tahan terhadap cekaman suhu diperlukan untuk memperluas area penanaman kentang di dataran menengah maupun dataran rendah.ABSTRACTPotato (Solanum tuberosum) is one of the important staple foods in the world. This plant is mostly cultivated in high-altitude regions with low temperatures. As the number of lands for potato cultivation is getting smaller due to land conversion. Potato cultivation in low-altitude regions with high temperatures yields low productivity. This study was designed to determine the effect of altitude and temperature changes on potato plants in the tuber formation phase. Control plants were planted in an area with an altitude of 2921 meters above sea level (m asl), with day/night temperatures (190C/120C). After the tuber formation phase, some plants were transferred to areas with an altitude of 115 m above sea level and day/night temperatures (300C/240C). Change in altitude with different temperatures resulted in shade avoidance, changes in the accumulation of biomass on plant stems, and yield reduction. This is due to decreasing sucrose content as photosynthesis assimilates, and chlorophyll content due to genetic factors and enzyme metabolism. Therefore, to support the increasing demand for potato commodities, breeding potato plants resistant to heat stress is needed to expand the potato planting area in middle or low altitudes. 
Pengaruh peningkatan suhu pada fase pembentukan umbi tanaman kentang (Solanum tuberosum) cv. Granola Pantouw, Carla Frieda; Hapsari, Betalini Widhi; Hastilestari, Bernadetta Rina
Jurnal AGRO Vol. 9 No. 1 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/18117

Abstract

Kentang (Solanum tuberosum) merupakan salah satu bahan makanan yang penting di dunia. Budidaya komoditas ini umumnya berada di dataran tinggi dengan suhu yang rendah. Jumlah lahan pertanian di dataran tinggi semakin kecil disebabkan antara lain karena alih fungsi lahan. Penanaman kentang di dataran yang lebih rendah menjadi kendala karena adanya peningkatan suhu. Penelitian ini dirancang untuk mengetahui pengaruh ketinggian tempat dan perubahan suhu terhadap tanaman kentang pada fase pembentukan umbi. Tanaman kontrol ditanam pada ketinggian 2921 meter diatas permukaan laut (m dpl) dengan suhu siang/malam (190C/120C). Setelah fase pembentukan umbi, sebagian tanaman dipindah ke daerah dengan ketinggian 115 m dpl dengan suhu siang/malam (300C/240C). Perubahan ketinggian tempat dengan suhu yang berbeda mengakibatkan shade avoidance, perubahan akumulasi biomasa pada batang tanaman dan penurunan hasil panen. Hal ini disebabkan karena penurunan hasil fotosintesa, sukrosa, serta kadar klorofil yang disebabkan oleh faktor genetik dan metabolisme enzim. Oleh karena untuk mendukung permintaan komoditas kentang yang semakin meningkat, pemuliaan tanaman kentang tahan terhadap cekaman suhu diperlukan untuk memperluas area penanaman kentang di dataran menengah maupun dataran rendah.ABSTRACTPotato (Solanum tuberosum) is one of the important staple foods in the world. This plant is mostly cultivated in high-altitude regions with low temperatures. As the number of lands for potato cultivation is getting smaller due to land conversion. Potato cultivation in low-altitude regions with high temperatures yields low productivity. This study was designed to determine the effect of altitude and temperature changes on potato plants in the tuber formation phase. Control plants were planted in an area with an altitude of 2921 meters above sea level (m asl), with day/night temperatures (190C/120C). After the tuber formation phase, some plants were transferred to areas with an altitude of 115 m above sea level and day/night temperatures (300C/240C). Change in altitude with different temperatures resulted in shade avoidance, changes in the accumulation of biomass on plant stems, and yield reduction. This is due to decreasing sucrose content as photosynthesis assimilates, and chlorophyll content due to genetic factors and enzyme metabolism. Therefore, to support the increasing demand for potato commodities, breeding potato plants resistant to heat stress is needed to expand the potato planting area in middle or low altitudes. 
Identification of Drought Tolerant Related Insertional Mutant Lines Using PEG 6000 Nugroho, Satya; Windiastri, Vincentia Esti; Widyajayanti, Dwi; Pantouw, Carla Frieda
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : BRIN

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Abstract

Drought is one of the most important abiotic stresses in rice (Oryza sativa) productivity. The development of drought tolerant cultivars are therefore highly desireable. We have developed insertional mutant based on the Japonica rice cv Nipponbare rice by transposons Ac/Ds insertions containing activation-tag and gene trap. Screening of the mutant population for drought tolerant related phenotypes is of our priority. The screening protocol based on PEG 6000 has been developed and was being used to screen 70 mutant lines tocharacterize their responds to the treatment based on different parameters (number of leaf, total weight, plant height, root length and number of germinating seeds). These characters were used to score the Degradation Index and Vigour Index. Results showed varyingresponds of the lines to the osmotic pressure. Some lines showing a good performance indicated by lower Degradation Index and higher Vigour Index have been identified. Some inconsistencies in the performances scored by both indices were thought to be due to seed quality.
Phenotypic Screening of Ds Transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya; Zannati, Anky; Situmorang, Apriadi; Windiastri, Vincentia Esti; Widyajayanti, Dwi; Pantouw, Carla Frieda; Astuti, Dwi; Indrayani, Sri; Rachmawati, Syamsidah; Mulyaningsih, Enung Sri; Santoso, Tri Joko; Trijatmiko, Kurniawan Rudi
Annales Bogorienses Vol. 15 No. 1 (2011): Annales Bogorienses
Publisher : BRIN

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Abstract

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice, combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller.