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All Journal Chemistry Indonesian Journal of Chemical Research Rumphius Pattimura Biological Journal
Seumahu, Cecillia Anna
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Engineering Environmental Science Immunology & microbiology

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Bioremediation of Pb and Cd Metal from Inner Ambon Bay Sediment Which Contaminated With Heavy Metal Using Aspergillus niger Male, Yusthinus T; Seumahu, Cecillia Anna; Malle, Dominggus
Indonesian Journal of Chemical Research Vol 7 No 2 (2020): Edisi Bulan Januari (Edition for January)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2020.7-yus

Abstract

Bioremediation is a method that use microorganism to extract heavy metal from contaminated waste. In this research Aspergillus niger was used to extract heavy metal such as Pb and Cd of marine sediment from Waiheru shore, Inner part of Ambon Bay, which was detected as significantly high heavy metal contaminated site among seven sites. Bioremediation were done using Aspergillus nigers to extract Pb and Cd metal from sediment, then their solubility being measured in filtrate media. Result shows that Cd metal were only detected 15 days after incubation while Pb were detected since the first day incubation. This result also showing the fluctuating solubility of Pb metal. It is suspected that this occurs due to biosorption ability of the fungi that being used which triggers metal accumulation in the cell structure. It is therefore can be concluded that Aspergillus niger can be used in bioremediation of sediment that are being contaminated by Pb and Cd heavy metals.
ANTIMICROBIAL POTENTIAL OF GARLIC FRESH (Allium sativum L.) AND LAKOR ONION (Allium ascalonicum L.) ON THE GROWTH OF Staphylococcus aureus Pagaya, Joseph; Lysay, Claudia Priscia; Seumahu, Cecillia Anna
RUMPHIUS Vol 3 No 2 (2021): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv3i2p030-033

Abstract

Garlic (Allium sativum L.) and shallot Lakor (Allium ascalonicum L.) local products are plants that are used as spices for various dishes and can be used as herbal medicine. For natural use, safe and easy to consume which can be seen through its potential as herbal medicine, a study was carried out to test the antimicrobial potential of garlic (Allium sativum L.) and Lakor shallot (Allium ascalonicum L.) juice on the growth of bacteria (Staphylococcus aureus). Lakor garlic and shallots are grated, the results of grated Lakor garlic and shallots are squeezed to produce 100% pure onion bulb juice. Lakor garlic and shallot juice was diluted with concentrations of 25%, 35%, 45%, 55%, 65%, 75%, 85% and 95%, respectively. Antimicrobial activity was tested by the diffusion method using a disk to measure the diameter of the clear zone. The results showed that garlic (Allium sativum L.) was able to inhibit the growth of Staphylococcus aureus which could be demonstrated by the clear zone diameter of 16.3 mm at a concentration of 35% and at a concentration of 65% the diameter of the clear zone exceeded the clear zone of the amoxycillin antibiotic. Lakor red onion (Allium ascalonicum L.) showed antimicrobial potential but was unable to damage the bacterial cell wall as indicated by the diameter of the clear zone of 7.7 mm at a concentration of 95%. There is potential for both onion bulbs but have different antimicrobial properties against the growth of Staphylococcus aureus.
Co-Authors DOMINGGUS MALLE Lysay, Claudia Priscia Pagaya, Joseph Yusthinus T. Male