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All Journal JPK: Jurnal Proteksi Kesehatan Journal of Medical Laboratory Research
rahmita, mellysa
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Isolation and Identification of Endophytic Bacteria in Blimbing Wuluh (Averrhoa blimbii) Leaves that have potential as Lactic Acid Bacteria Lestari, Kustiasih; Rahmita, Mellysa; Fitria, Fitria
JURNAL PROTEKSI KESEHATAN Vol 13 No 1 (2024): JPK: Jurnal Proteksi Kesehatan
Publisher : Poltekkes Kemenkes Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36929/jpk.v13i1.868

Abstract

Lactic acid bacteria (LAB) are widely recognized for their numerous benefits. Apart from their medicinal properties, they can also serve as additional ingredient in food. Their high acidity is believed to be a plant's defense mechanism associated with endophytic microbes, which greatly benefit plant growth. Lactic acid bacteria (LAB) can offer advantages to humans and are widely distributed in nature. However, the collection of indigenous lactic acid remains limited, necessitating exploration of lactic acid from Indonesia's natural environment. This study aims to isolate and identify endophyt bacteria in the leaves of Averrhoa bilimbi (Blimbing wuluh) that potentially serve as sources of LAB. The research employed purposive sampling for sample collection and laboratory experiments. Results revealed that Blimbing wuluh leaves contain endophytic bacteria which have the potential to be lactic acid bacteria, with positive carbohydrate tests result to ferment lactose, sucrose, and glucose. The results of the Lactic Acid Bacteria test were positive on MRSB media and MRSA media. The results of the endophytic bacteria isolation characterization test succeeded in identifying 3 isolates from Blimbing wuluh (Averrhoa blimbii) which had the potential to be Lactic Acid Bacteria (LAB).
Isolasi dan Identifikasi Bakteri Endofit pada Batang dan Daun Tanaman Songgolangit (Tridax procumbens (Lour.)) Hamtini, Hamtini; Nurhati, Wida; Rahmita, Mellysa; Trisna, Citra; Rahmawati, Juli; Shufiyani, Shufiyani
Journal of Medical Laboratory Research Vol. 1 No. 1 (2022): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.141 KB) | DOI: 10.36743/jomlr.v1i1.430

Abstract

Bakteri endofit adalah bakteri yang hidup di dalam jaringan tumbuhan secara simbiotik dengan membentuk koloni selama periode tertentu dari siklus hidupnya dan dapat menghasilkan metabolit sekunder. Salah satu tanaman yang memiliki potensi untuk dikembangkan bakteri endofit dari obat tradisional adalah tanaman Songgolangit (Tridax procumbens (Lour)). Tanaman ini merupakan tanaman herba menahun yang digunakan sebagai tanaman obat yang hampir seluruh bagian tanamannya dapat digunakan. Penelitian ini bertujuan untuk mengetahui ada atau tidaknya bakteri endofit serta mengetahui jenis bakteri endofit yang terdapat pada batang dan daun tanaman Tridax procumbens. Bakteri endofit diisolasi dari batang dan daun tanaman Tridax procumbens menggunakan metode sterilisasi permukaan kemudian dikulturkan pada media Trypticase Soy Agar (TSA) + antibiotik nistatin 100 ppm lalu diidentifikasi melalui pewarnaan Gram serta dilakukan preservasi bakteri endofit pada agar miring Trypticase Soy Agar (TSA) dan gliserol 40%. Hasil penelitian diperoleh 25 isolat bakteri endofit yang terdiri dari 16 isolat bakteri pada batang dan 9 isolat bakteri pada daun tanaman Tridax procumbens. Isolat bakteri endofit yang diperoleh dari pengamatan Gram didapatkan hasil 17 isolat bakteri endofit Gram (+) dan 8 isolat bakteri endofit Gram (-). Jumlah bakteri endofit di dalam tanaman tidak dapat ditentukan secara pasti, namun bakteri endofit dapat dideteksi dengan mengisolasi pada media agar dan Teknik isolasi yang tepat.
Co-Authors Fitria Fitria Lestari, Kustiasih Nurhati, Wida Rahmawati, Juli Shufiyani, Shufiyani Trisna, Citra