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All Journal Indonesian Journal of Biotechnology and Biodiversity Journal of Stem Cell Research and Tissue Engineering
Naroeni, Aroem
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Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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SEL FEEDER DAN CONDITIONED MEDIUM UNTUK KULTUR SEL PUNCA EMBRIONIK MENCIT SEBAGAI MODEL UNTUK PROPAGASI SEL PUNCA EMBRIONIK Naroeni, Aroem
Indonesian Journal of Biotechnology and Biodiversity Vol 1, No 2 (2017): Indonesian Journal of Biotechnology and Biodiversity
Publisher : Universitas Esa Unggul

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Abstract

AbstractStem cell is very promising for regenerative medicine. Unfortunately, the sources of stem cells are limited. Umbilical cord, bonne marrow are now the most used as stem cell source. Many techniques are now being developed to isolate, propagate and differentiate stem cells. The development of induced-pluripotent stem cells that reprogram adult cells to stem cells are being developed as well. In this research we have developed stem cell culture for the propagation of stem cells by using feeder cells and conditioned medium. Stem cells were isolated from mice embryonic  13 days after copulation. We obtained bulks of stem cells surrounded with mouse fibroblast cells. Stem cells require this mouse fibroblast cells as a feeder and conditioned medium, Medium that have been added with supernatant of 3 days cell culture. Keywords : stem cell, conditioned medium, induced-pluripotent stem cell AbstrakSel punca sangat menjanjikan dalam bidang kedokteran regeneratif karena dapat menyembuhkan berbagai penyakit karena kerusakan jaringan. Meskipun begitu, sumber sel punca yang dapat digunakan untuk keperluan tersebut sangat terbatas. Sel darah pusat dan sumsum tulang belakang saat iniadalah merupakan sumber sel punca yang paling banyak digunakan. Beberapa macam teknik sedang dikembangkan untuk mengisolasi, propagasi dan differensiasi sel punca. Perkembangan induced-pluripotent stem cell (iPS) untuk propagasi sel punca juga sedang dikembangkan. Pada penelitian ini, kami mengembangkan kultur sel untuk propagasi sel punca dengan menggunakan sel feeders dan conditioned medium. Sel punca diisolasi dari embrio mencit 13 hari setelah kopulasi. Setelah dikultur dalam media tersebut maka diperoleh sekelompok sel punca yang dikelilingi oleh sel fibroblas. Sel punca dapat dikembangkan dan dipertahankan sifat kepuncaannya dengan menggunakan sel fibroblas sebagai sel feeder dan penambahan conditioned medium pada media kulturnya. Kata kunci : sel punca, conditioned medium, induced-pluripotent stem cell
BIOSCAFFOLD FROM MOUSE EMBRYONIC FIBROBLAST MAINTAINS THE PLURIPOTENCY OF MOUSE EMBRYONIC STEM CELLS Jannah, Rifqah Mifthahul; Naroeni, Aroem; Novianti, Titta
Journal of Stem Cell Research and Tissue Engineering Vol. 7 No. 1 (2023): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v7i1.38140

Abstract

Cell culture using a 3D method provides Various cell culture strategies have been developed using synthetic or biological materials; most existing publications use many reagents. Bioscaffold from mouse embryonic fibroblast (MEF) enhances cell attachment, interaction, and production of growth factors. Since bioscaffolds could maintain and stimulate pluripotency of stem cells, we conducted this study to prove bioscaffold function. Bioscaffold was prepared from MEF cultured in  DMEM complete medium supplemented with dextran sulfate and L-ascorbic acid to increase extracellular matrix production. This medium acts as an embryo stem cell (ESC) culture medium. We used a Tali-cytometer to identify and quantify stem cells based on Sox2 and Oct4 proteins, markers of stemness. ESC culture using bioscaffold maintained the pluripotency of ESC as indicated by the presence of  Oct 4 and Sox2 as ESC markers compared to MEF culture. From this research, the bioscaffold from MEF can be developed as media for ESC to improve propagation. Furthermore, it is a model for tissue engineering and in vitro organ development.
Co-Authors Jannah, Rifqah Mifthahul Novianti, Titta