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All Journal EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Ismanto, .
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Mathematics

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RESPON PERTUMBUHAN STEK PUCUK DAN STEK BATANG GANITRI (Elaeocarpus ganitrus Roxb.) TERHADAP HORMON PERTUMBUHAN Filopor, .; Ismanto, .; Prameswari, Diana
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 23, No 1 (2023): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v23i1.6234

Abstract

Ganitri (Elaeocarpus ganitrus Roxb) is a fast-growing tree species that is widely found in Indonesia, Malaysia, Myanmar, and Thailand. Ganitri seedlings’ generative propagation takes a long time because the seed coat is very hard, causing difficulty to germinate, therefore it is conducted vegetatively using stem and shoot cuttings with hormone treatment. The research objective was to obtain growth response of shoot cuttings and ganitri stem cuttings to hormone administration. The research was conducted in Silviculture greenhouse at Center for Standardization of Instruments for Sustainable Forest Management in Bogor. It used a Completely Randomized Design with a factorial pattern consisting of 2 factors. The first factor is cutting material: shoot cuttings and stem cuttings. The second factor is phytohormones: Rootone-F and coconut water. The results showed shoot cuttings with Rootone-F treatment gave the best growth compared to other treatment combinations. This was indicated by percent growing cuttings, percent rooted cuttings, root length, shoot wet weight, root wet weight, shoot dry weight and root dry weight which were higher with values of 63.33%, 53.0%, 8.10 cm, 2.92 grams, 0.28 grams, 1.33 grams, and 0.21 grams respectively. Thus, shoot cuttings survival with Rotoone-F hormone had a higher result than stem cuttings, reaching 63.33%.
AKLIMATISASI PLANLET JAHE PUTIH BESAR (Zingiber officinale) HASIL PERBANYAKAN MELALUI KULTUR IN VITRO Ismanto, .; Djarot, Prasetyorini; Pertiwi, Anindita Aulya
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 24, No 2 (2024): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v24i2.10881

Abstract

Giant ginger is a rhizome plant known as a spice and herbal medicine originating from South Asia, for preventing or treating various diseases because it contains gingerol. To increase the production of giant ginger plant seeds besides cultivation it can be done by utilizing in vitro culture techniques. The aim and novelty of the research is to obtain a new in-vitro medium that is suitable for producing ginger plant seeds with good and uniform quality.The research was carried out in January-December 2023. The explants used were giant ginger culture in vitro collected from the Tissue Culture Laboratory, Center for Biotechnology and Agricultural Genetic Resources. The explants were grown on MS media with the addition of a combination treatment of growth regulators BAP (0, 1,  and 5 mg L-1) and TDZ (0.0, 0.1 and 0.2 mg L-1) then continued with the acclimatization process. The experimental design used was a Completely Randomized Factorial Design. The research results showed that in in-vitro the combination of 1.0 mg L-1 BAP treatment without the addition of TDZ was chosen as the ZPT concentration treatment level because it produced the best response to the growth of large white ginger explants. And in ex-vitro, the treatment combination of BAP 1.0 + TDZ 0.1, BAP 1.0 mg L-1 without the addition of TDZ, and control was chosen as the ZPT concentration treatment level because it produced the best response to the growth of giant ginger explants.
Co-Authors Diana Prameswari, Diana Djarot, Prasetyorini Filopor, . Pertiwi, Anindita Aulya