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All Journal EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup International Journal of Health, Medicine, and Sports
Pertiwi, Anindita Aulya
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Environmental Science Health Professions Mathematics Medicine & Pharmacology Nursing Public Health

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AKLIMATISASI PLANLET JAHE PUTIH BESAR (Zingiber officinale) HASIL PERBANYAKAN MELALUI KULTUR IN VITRO Ismanto, .; Djarot, Prasetyorini; Pertiwi, Anindita Aulya
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 24, No 2 (2024): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekologia.v24i2.10881

Abstract

Giant ginger is a rhizome plant known as a spice and herbal medicine originating from South Asia, for preventing or treating various diseases because it contains gingerol. To increase the production of giant ginger plant seeds besides cultivation it can be done by utilizing in vitro culture techniques. The aim and novelty of the research is to obtain a new in-vitro medium that is suitable for producing ginger plant seeds with good and uniform quality.The research was carried out in January-December 2023. The explants used were giant ginger culture in vitro collected from the Tissue Culture Laboratory, Center for Biotechnology and Agricultural Genetic Resources. The explants were grown on MS media with the addition of a combination treatment of growth regulators BAP (0, 1,  and 5 mg L-1) and TDZ (0.0, 0.1 and 0.2 mg L-1) then continued with the acclimatization process. The experimental design used was a Completely Randomized Factorial Design. The research results showed that in in-vitro the combination of 1.0 mg L-1 BAP treatment without the addition of TDZ was chosen as the ZPT concentration treatment level because it produced the best response to the growth of large white ginger explants. And in ex-vitro, the treatment combination of BAP 1.0 + TDZ 0.1, BAP 1.0 mg L-1 without the addition of TDZ, and control was chosen as the ZPT concentration treatment level because it produced the best response to the growth of giant ginger explants.
Antioxidant Activity, Toxicity Test and Antimicrobal Test of Streptococcus mutans and Candida albicans Extract Of Ultrasound Assisted Extraction of Java Chili (Piper retrofactum Vahl.) Leaves Djarot, Prasetyorini; Pertiwi, Anindita Aulya; Yulianita; Dewi, Fitria; Safira, Nurul; Firda, Varisa Yulia
International Journal of Health, Medicine, and Sports Vol. 3 No. 3 (2025): International Journal of Health, Medicine, and Sports
Publisher : Corespub

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46336/ijhms.v3i3.239

Abstract

P. retrofractum leaves are widely cultivated by the community to be used as traditional medicine, this is because of its secondary metabolite content which has several bioactive components such as steroids, flavonoids, alkaloids, saponins, tannins and has pyhtol and piperine compounds as dominant compounds that have antimicrobial potential. This study aims to determine flavonoid content, antioxidant activity and cytotoxic test of P. retrofractum leaf extract. The extraction method used was Ultrasound Assisted Extraction (UAE) with 3 solvents of different polarity, namely n-Hexan, ethyl acetate and 70% ethanol. Determination of flavonoid content using UV-Vis spectrophotometric method, determination of antioxidant activity using DPPH method and cytotoxic activity test using Brine Shrimp Lethality Test (BSLT) method. Antibacterial activity test was conducted by measuring the Minimum Inhibitory Concentration (KHM) and Diameter of Inhibitory Area (DDH). KHM test was done by agar dilution method, DDH test by disc paper diffusion method. The results of the extraction of P retrofractum leaves produce the highest yield is ethanol extract which is 9.92%. The result of determining the highest flavanoid content is 70% ethanol extract at 1.2702%. The strongest antioxidant activity is 70% ethanol extract with an IC 50value of 40.7475 ppm with a very strong category. The results of the cytotoxic test showed that the three types of extracts were toxic because they had LC50 less than 1000 µg/mL. The best KHM result for S. mutans is 10% n-hexane extract and 10% ethyl acetate and for Candida albicans is 5% n-hexane extract. The best DDH of S. mutans was 20% n-hexane extract with a value of 17.37 mm approaching Susceptible (Intermedate) and the best DDH of Candida albicans was obtained by 20% n-Hexan extract with an average DDH of 17.12 mm approaching Susceptible (Intermedate).
Co-Authors Dewi, Fitria Djarot, Prasetyorini Firda, Varisa Yulia Ismanto, . Safira, Nurul Yulianita