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All Journal Indonesian Journal of Natural Pigments
Kurniawan, Jovine M
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Chemistry Other

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Simple Photometer Development For Educational Purposes in Natural Pigment Analysis Kurniawan, Jovine M; Adhiwibawa, Marcelinus AS
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.17

Abstract

In this paper we will discuss the making of the simple photometer instrument to measure the absorbance of natural pigment crude extract. To make simple photometer, cuvette holder was printed using 3D Printing Machine, the development of simple photometer instrument also uses some components such as LED light (633 nm) as the light source, Photodiode-TSL250 as a detector and a variable resistor to set the initial intensity of light source. In this development, Arduino Uno was also used as USB data acquisition device to capture the signal from the instrument. The results of sample measurements between simple photometer instrument and UV-Vis 1800 spectrophotometer showed 98% regression coefficient of determination and simple photometer has a LOD and LOQ absorbance at 0.0267 and 0.0344. Simple photometer instrument was able to show similar response as commercial instrument. Through the development of simple photometer, student can easily understand the working principles of a spectrophotometer in natural pigment analysis that difficult to studied in detailed before.
Intensity Ratio of LH2 Complexes from Rhodopseudomonas palustris and Rhodobacter sphaeroides for Purity Determination Prihastyanti, Monika NU; Kurniawan, Jovine M; Yusuf, Melisa M; Azmi, Sherly S; Ilmi, Mustafavi C; Indarto, Michelle L; Hoetomo, Stefan C; Wijaya, Melina E; Jatiyati, Greta; Brotosudarmo, Tatas H.P.; Heriyanto, Heriyanto
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.13

Abstract

Purification procedures to obtain light-harvesting complexes were performed on two photosynthetic bacteria, i.e. Rhodospseudomonas (Rps.) palustris and Rhodobacter (Rb.) sphaeroides. In this study, purification was initially carried out using series of centrifugation towards the bacteria cell to acquire chromatophore of the bacteria. Application of detergent (lauryldimethylamine oxide, or LDAO) to the chromatophore enabled the extraction of membrane containing photosynthetic apparatus. Further purification involved the application of sucrose density gradient centrifugation and ion exchange chromatography. The purity of collected LH2 complexes can be determined by calculating the ratio of AB850 : Aprotein. The resulting LH2 complexes from both bacteria showed relatively high ratio suggesting the purity of the complexes.
Co-Authors Adhiwibawa, Marcelinus AS Azmi, Sherly S Hoetomo, Stefan C Ilmi, Mustafavi C Indarto, Michelle L Jatiyati, Greta Prihastyanti, Monika NU Tatas H.P. Brotosudarmo Wijaya, Melina E Yundari, Yundari Yusuf, Melisa M