Article Per Year (5 Year)
p-Index From 2020 - 2025
1.978
P-Index
This Author published in this journals
All Journal Natural Science: Journal of Science and Technology ILMU KELAUTAN: Indonesian Journal of Marine Sciences JURNAL KIMIA SAINS DAN APLIKASI Media Penelitian dan Pengembangan Kesehatan AL KAUNIYAH Proceeding of the Electrical Engineering Computer Science and Informatics Indonesian Journal of Chemistry Journal of Epidemiology and Public Health Indonesian Journal of Natural Pigments Jurnal Aisyah : Jurnal Ilmu Kesehatan JURNAL REKAYASA KIMIA & LINGKUNGAN
Tatas H.P. Brotosudarmo
Department Of Food Technology, Universitas Ciputra Surabaya
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Electrical & Electronics Engineering Energy Engineering Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary Other

Published : 20 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 20 Documents
Search

 1   2 
Economically Potential Pigments from Marine Blue-Green Algae for the Application in Food and Health Salim, Katarina Purnomo; Chandra, Rosita Dwi; Heriyanto, Heriyanto; Susilaningsih, Dwi; Limantara, Leenawaty; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 1 No 2 (2019): Agustus 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.2.37

Abstract

Current efforts to explore the potency of marine resources have been made to promote economic development in Indonesia, especially in the industrial sector. Marine cyanobacteria such as Arthospira sp. and Scytonema sp. are two potential candidates of the blue-green algae that can be used in the application for food and health industries. This study focused on the identification of the composition of the dominant chlorophylls and carotenoids. The results showed the presence of Zeaxanthin, Chlorophyll a, and β-carotene. The economic potency of those dominant pigments will be reviewed in this article.
Multispectral Imaging with Raspberry Pi for Assessment of Plant Health Status Bosse, Jan Lukas; Adhiwibawa, Marcelinus A. S.; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 1 No 2 (2019): Agustus 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.2.30

Abstract

Non-destructive measurement of plant chlorophyll concentration using the normalized difference vegetation index (NDVI) has long been standard practice to determine the plant health status. This is, because the NDVI value is correlated with the chlorophyll concentration which in turn is highly correlated with other vital plant parameters such as nitrogen and magnesium concentration. Initially the NDVI values were obtained from satellite imagery and thus could only be used to assess the health status of bigger ecosystems like forests and crop fields. With the introduction of handheld chlorophyll meters like the Chlorophyll Meter SPAD-502 Plus made by Konica Minolta, the same principle could be used to determine the chlorophyll concentration of single leaves. However, these devices still have one major shortcoming: They can only measure the chlorophyll concentration on one single spot on the leaf at a time. But depending on the species the chlorophyll concentration tends to vary significantly over the leaf. To overcome this shortcoming, we developed our PlantAnalyzer which offers better spatial resolution of the NDVI values and hence the chlorophyll concentration. Its technical realization and precision shall be elaborated in the following article.
Isolation, Encapsulation, Stability and Characteristics of Thylakoid from Suji Leaves (Pleomele angustifolia) as Natural Food Coloring Agent Chandra, Rosita Dwi; Indrawati, Renny; Heriyanto, Heriyanto; Brotosudarmo, Tatas H.P.; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 1 No 2 (2019): Agustus 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.2.53

Abstract

Suji (Pleomele angustifolia) is one kind of Indonesian typical plants which can be used as natural green food coloring agent. The susceptibility of natural pigment to external environment forces the protection in order to prolong its shelf life. Encapsulation has been known in the art of food preparation to provide protection for several ingredients including food coloring agent. The objective of this study was to observe the method for isolation and encapsulation of thylakoid, and to investigate the stability and characteristics of thylakoid of suji leaves encapsulated in maltodextrin during dark storage at 30 °C, 45 °C, and 60 °C. The degradation of the encapsulated pigments was identified through chromametric analysis which resulted in the increase of L* (lightness), a* (redness), and b* (yellowness) values. In addition, it was also indicated by the decrease of total chlorophyll (TC) which was determined using spectrophotometer. Chromatography analysis confirmed the presence of four major peaks in the fresh encapsulated thylakoid powder and five major peaks in the encapsulated thylakoid powder stored at the highest temperature (60 °C), with Chl a as the dominant pigments in both powder. The vivid green powder was able to preserve its color without any obvious change to an untrained eye up to 60 d of storage at 30 °C, becoming a promising ingredient to replace the synthetic colorants.
KARAKTERISASI ANTOSIANIN BUAH MURBEI SPESIES MORUS ALBA DAN MORUS CATHAYANA DI INDONESIA Sitepu, Rehmadanta; Heryanto, Heryanto; Brotosudarmo, Tatas H.P.; Limantara, Leenawaty
Natural Science: Journal of Science and Technology Vol 5, No 2 (2016): Volume 5 Number 2 (August 2016)
Publisher : Univ. Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.355 KB)

Abstract

Buah murbei kaya antioksidan karena kandungan antosianin yang banyak. Sayangnya,  pemanfaatannya sebagai sumber antioksidan di Indonesia masih  minim. Pemanfaatan tanaman murbei hanya sebatas daun yang dijadikan pakan ternak dan teh. Penelitian ini bertujuan untuk melakukan karakterisasi antosianin buah murbei spesies Morus alba dan Morus cathayana. Karakterisasi antosianin ekstrak buah murbei Morus cathayana dan Morus alba dilakukan menggunakan pelarut 0,1% asam klorida (HCl)  dalam metanol dan dianalisis menggunakan spektrofotometer UV-tampak dan Kromatografi Cair Kinerja Tinggi (KCKT). Hasil serapan spektra UV-tampak yang diperoleh dari ekstrak kasar menunjukkan spektra serapan ekstrak buah M. cathayana memberikan nilai yang lebih tinggi ± 1,3 a.u dibandingkan serapan yang diperoleh dari ekstrak kasar buah M. alba dengan serapan ± 0,4 a.u. Nilai total antosianin yang diperoleh dari M. cathayana sebanding dengan serapan ekstrak segarnya, yaitu 40,39 ± 7,64 mg/g berat kering dibandingkan  M. alba yang memiliki nilai total antosianin 11,57 ± 3,02 mg/g berat kering. Hasil kromatogram dari KCKT menggunakan kolom XR-ODS dengan pengelusi A adalah 0,1% asam formiat dalam asetonitril dan pengelusi B adalah 0,1% asam formiat dalam air menunjukkan baik M. cathayana maupun M. alba memiliki dua pigmen antosianin dominan. Walaupun bergitu, intensitas kromatogram M. cathayana lebih tinggi dibandingkan intesitas pada M. alba. Nilai intensitas M. cathayana adalah 50 mAU untuk waktu tambat 7,86 menit dan 15 mAU untuk waktu tambat 8,38 menit. Nilai intensitas M. alba adalah 10 mAU untuk waktu tambat 7,35 menit dan 3 mAU untuk waktu tambat 7,76 menit. Dua antosianin dominan pada M. alba dan M. cathayana  diprediksi  merupakan sianidin-3-O-glukosida dan sianidin-3-O-rutinosida.
Intensity Ratio of LH2 Complexes from Rhodopseudomonas palustris and Rhodobacter sphaeroides for Purity Determination Prihastyanti, Monika NU; Kurniawan, Jovine M; Yusuf, Melisa M; Azmi, Sherly S; Ilmi, Mustafavi C; Indarto, Michelle L; Hoetomo, Stefan C; Wijaya, Melina E; Jatiyati, Greta; Brotosudarmo, Tatas H.P.; Heriyanto, Heriyanto
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.13

Abstract

Purification procedures to obtain light-harvesting complexes were performed on two photosynthetic bacteria, i.e. Rhodospseudomonas (Rps.) palustris and Rhodobacter (Rb.) sphaeroides. In this study, purification was initially carried out using series of centrifugation towards the bacteria cell to acquire chromatophore of the bacteria. Application of detergent (lauryldimethylamine oxide, or LDAO) to the chromatophore enabled the extraction of membrane containing photosynthetic apparatus. Further purification involved the application of sucrose density gradient centrifugation and ion exchange chromatography. The purity of collected LH2 complexes can be determined by calculating the ratio of AB850 : Aprotein. The resulting LH2 complexes from both bacteria showed relatively high ratio suggesting the purity of the complexes.
Thermal Degradation Kinetics of Encapsulated Palm Carotenes Using Different Combination of Wall Materials Hong, Khoo Mun; Indrawati, Renny; Brotosudarmo, Tatas HP; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 2 No 1 (2020): February 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.1.21

Abstract

Palm oil is known as one of the richest sources of carotenes. Carotenes concentrate could be prepared from crude palm oil and possessing potency to be used as nutraceutical materials. Due to the susceptibility of carotenes against heat, the encapsulation procedure might be applied to provide protections. The present study was aimed to learn the influence of using different combination of wall materials toward the thermal degradation kinetics of palm carotenes. Maltodextrin was used as primary wall material, being combined with various surface-active agents, i.e. Tween-80 (A), Tween-20 (B), and Arabic gum (C). The carotenes concentrate was encapsulated through emulsification steps, and the vivid yellow powder was obtained after freeze drying process. Then, encapsulated palm carotenes were stored in 200C, 350C, and 500C for several days, and the color, carotenes spectrum, as well as water content were monitored periodically. The kinetics of thermal degradation of carotenes were estimated using Arrhenius equation. The results showed that the use of different combination of wall materials exhibited distinct degradation rate. The combination of maltodextrin and Tween-80 gave preferable protection compared to the others, being half-degraded after 51 days in 200C.
Humans’ Body Immunity and Natural Pigments Indrawati, Renny; Limantara, Leenawaty; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.26

Abstract

The emerging pandemic of Corona Virus Disease (COVID-19) has attracted special concerns regarding the case of very contagious viral infection. Besides the profound waiting toward vaccine development, public awareness was also addressed into the effort of increasing humans’ body immunity. Nowadays, the people are encouraged to consume adequate amount of vitamin C and increase the consumption of fruits and vegetables. In addition, various kinds of food supplements are offered and claimed to give us protection and prevention against viral infection by boosting our immune system. However, the communities are actually lack of proper information regarding the true mechanism of immune system. How is the infection developed in our body? How does the immune system work? Could it be modulated by consuming the bioactive compounds? Here, the stages of infection, the immune system, as well as the role of natural pigments to support the humans’ body immunity will be briefly discussed.
Characterization of Tambjamines Pigment from Marine Bacterium Pseudoalteromonas sp. PM2 Indigenous from Alor Island, Indonesia Setiyono, Edi; Adhiwibawa, Marcelinus Alfasisurya Setya; Prabowo, Matheus Randy; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 3 No 1 (2021): February 2021
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2021.03.1.16-23

Abstract

Pigments from marine bacteria have attracted the attention for scientists because of their extensive applications and currently exploration of new pigment sources from marine bacteria is still ongoing. Recently, we have successfully isolated six new yellow-pigmented marine bacteria, strain PS2, PM2, SB11, SB13, SB21, and SB23, isolated from seawater from different sampling sites on Alor Island, Indonesia. The UV−Vis and FTIR spectra of the crude pigment extracts of the six strains showed the characteristics of tambjamines, a group of yellow pigments commonly found in nudibranchs and bryozoans. Moreover, separation and characterization of crude tambjamines extract resulted in five different types of tambjamine with maximum absorbance at the wavelength of 374−392 nm. Based on the analysis of 16S rRNA gene sequences, strain PM2 was closely related to several species in genus Pseudoalteromonas with a similarity of more than 99%. Strain PM2 was designed as Pseudoalteromonas sp. PM2 with accession number LC505058. So far, only two marine bacteria have been known to produce tambjamine and they are from genus Pseudoalteromonas. Our new finding indicated that in the group of marine bacteria, tambjamine might be only synthesized by members from genus Pseudoalteromonas.
Marine bacterium Seonamhaeicola algicola strain CC1 as a potential source for the antioxidant carotenoid, zeaxanthin Tatas Hardo Panintingjati Brotosudarmo; Edi Setiyono; Koichiro Awai; Delianis Pringgenies
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 4 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.4.215--224

Abstract

Currently, there are only six species in the genus Seonamhaeicola, i.e., Seonamhaeicola aphaedonensis, S. algicola, S. marinus, S. acroporae, S. maritimus, and S. sediminis. These bacteria have typical yellow or orange color. Among the identified strains, only S. marinus that had been reported to have a yellow polyene flexirubin pigment. However, the presence of carotenoid pigments has not been reported in this genus. Recently, we successfully isolated a new strain, S. algicola strain CC1, bacterium that was found in association with a red seaweed, Halymenia sp., collected from the coast of South Malang, Indonesia. The strain was grown well in the Zobell marine agar 2216E producing yellowish pigments. According to the 16S rRNA sequencing analysis and BLAST search, the strain is closely related to S. algicola strain Gy8, with 99.78% identity. The pigment composition was separated and analyzed by a high-performance liquid chromatography with tandem mass spectrometry detection (HPLC-MS/MS) and the strain was found to produce zeaxanthin as the major component, which appeared at a retention time (tR) of 28.89 min, showing a typical mass spectrum with a molecular ion at m/z 568.5 [M]+ and four product ions at m/z 261.4 [M−307]+, 476.6 [M−92]+, 429.3 [M−139]+, and 536.5 [M− 32]+. Other carotenoids, including zeaxanthin cis isomers, β-cryptoxanthin, β-carotene cis isomer, and β-carotene, are as minor components. The novel and noteworthy finding of this report is the identification of a Seonamhaeicola species that produces carotenoids and can be used as a source of zeaxanthin.
Estimasi Produk Degradasi Ekstrak Kasar Pigmen Alga Merah Kappaphycus alvarezii (Doty) Doty Varian Merah, Coklat, dan Hijau: Telaah Perbedaan Spektrum Serapan Helly de Fretes; A.B. Susanto; Budi Prasetyo; Heriyanto Heriyanto; Tatas H.P. Brotosudarmo; Leenawaty Limantara
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 1 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.571 KB) | DOI: 10.14710/ik.ijms.17.1.31-38

Abstract

Penelitian ini bertujuan untuk mengestimasi produk degradasi pigmen ekstrak kasar alga merah Kappaphycus alvarezii (Doty) Doty varian merah, coklat dan hijau yang terbentuk selama perlakuan iradiasi dan pemanasan, melalui perbedaan spektrum serapannya. Ketiga varian diekstraksi menggunakan 100% metanol. Uji fotostabilitas ekstrak pigmen dilakukan dengan iradiasi menggunakan lampu Volpi intralux 4100 pada intensitas cahaya 39300 lux, 56700 lux dan 76400 lux.  Lama waktu penyinaran 0, 5, 10, 15, dan 20 menit. Sedangkan uji termostabilitas  dilakukan pada suhu 25 dan 90 ° C selama 0, 3, 6 24, dan 48 jam. Pola spektra diukur pada panjang gelombang 300-800 nm sebelum dan sesudah perlakuan dengan spektrofotometer UV-Tampak Shimadzu 1700. Data dianalisis dengan SPINA Versi 3, untuk memperoleh intensitas maksimum dan perbedaan spektra serapan. Hasil penelitian menunjukkan bahwa produk degradasi pigmen ekstrak kasar alga merah K. alvarezii dapat diidentifikasi melalui spektrum serapan dan hasil perbedaan spektra serapan. Isomer cis karotenoid, karotenoid tidak berwarna, dan feofitin a diestimasi sebagai produk degradasi yang terbentuk selama perlakuan iradiasi dengan intensitas cahaya 76400 lux dan perlakuan pemanasan pada suhu 90°C selama 48 jam. Penurunan intensitas warna larutan pigmen juga mengindikasikan terbentuknya produk degradasi selama perlakuan. Kata kunci: Kappaphycus alvarezii, perbedaan spektrum serapan, produk degradasi The aims of this study were to estimate the degradation products of crude pigment extracts from red, brown, and green varieties of red alga Kappaphycus alverezii (Doty) Doty that formed during irradiation and heating treatment from their difference absorption spectra. All three variants were extracted using 100% methanol. The photostability assay of crude pigment extracts was conducted by irradiating the crude pigment extracts with a day light lamp (Volpi, Intralux 4100) at 39300 lux, 56700 lux and 76400 lux light intensity during 0, 5, 10, 15, and 20 minutes treatment, whereas the thermostability assay was performed at 25 and 90 °C for 0, 3, 6 24, and 48 hours. The absorption spectra of the crude pigment extract before and after each treatment were monitored successively at 300-800 nm using MultiSpec 1501 UV-VIS spectrophotometer. Data were analyzed with spina Version 3, to obtain maximum intensity and the difference absorption spectra. The results showed that the  degradation products of crude pigment extracts from red alga K. alvarezii could be identified by absorption spectra and difference absorption spectra. Cis isomers carotenoids, colorless carotenoids, and feofitin-a were estimated as degradation product formed during the irradiation treatment with 76400 lux of light intensity and heating treatment at a temperature of 90 ° C for 48 hours. Decrease in the intensity of the color of pigment solution also indicates the formation of degradation products during treatment.Key words: Kappaphycus alvarezii, difference absorption spectra, degradation products
Co-Authors A.S. Adhiwibawa, Marcelinus Adhiwibawa, Marcelinus A. S. Adhiwibawa, Marcelinus Alfasisurya Setya Agus Suryanto Anam, Syaiful Antonius Budi Susanto Azmi, Sherly S Bosse, Jan Lukas Budi Prasetyo Chandra Ayu Siswanti David Sukardi Kodrat Dece Elisabeth Sahertian Delianis Pringgenies Devi Natalia Chrisdiyanti DWI SUSILANINGSIH Edi Setiyono Edi Setiyono Fanny Tanzilia, May Gonaldy, Vincent Aurelius Hana Susanti Maleta Helly de Fretes Hendrik O. Lintang Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heriyanto Heryanto Heryanto Hoetomo, Stefan C Hong, Khoo Mun Ilmi, Mustafavi C Indarto, Michelle L Indriatmoko Indriatmoko Ivan C. Onggara Jatiyati, Greta Jodiawan Jodiawan Jovine Marcella Kurniawan Kestrilia Rega Prilianti Koichiro Awai Kurniawan, Jovine M Leenawaty Limantara Lia Hapsari Lidwina Angelica Soetantijo Maleta, Hana Susanti Marcelinus A.S. Adhiwibawa Melisa Megawati Yusuf Minarni Wartiningsih Mitha Ayu Pratama Handojo Monika Nur Utami Prihastyanti Ni Njoman Juliasih Nur Vi’atin Onggara, Ivan C. Prabowo, Matheus Randy Prihastyanti, Monika NU Renny Indrawati Renny Indrawati, Renny Richard J. Cogdell Rosita Dwi Chandra Rosita Dwi Chandra, Rosita Dwi Salim, Katarina Purnomo Silitonga, Hanna Sitepu, Rehmadanta Sugiyatmi, Tri Astuti Suryanto, Agus Tanzilia, May Fanny Wijaya, Melina E Yundari, Yundari Yusuf, Melisa M