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All Journal YARSI Medical Journal JOURNAL LA MEDIHEALTICO
A. Rantam, Fedik
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Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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DETEKSI INTERLEUKIN-10 (IL-10) PADA MICE BALB/C YANG DIINFEKSI DENGAN VIRUS DENGUE: TELAAH PATOGENESIS ANTARA TEORI INFEKSI SEKUNDER DAN VIRULENSI Yusuf, Iwang; Konthen, P.G.; A. Rantam, Fedik
Jurnal Kedokteran YARSI Vol 13, No 1 (2005): JANUARI - APRIL 2005
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.106 KB) | DOI: 10.33476/jky.v13i1.1053

Abstract

Since the pathogenesis/immunopathogenesis of vasculopathy plasma leakage in DHF/ DSS was not clearly understood, this study was conducted. Considering the equilibrium between pro and anti inflammatory cytokine in homeostatic process in inflammation, this study was aimed to; first, detect the increase of secretion of IL-10 of immune responses of Mice Balb/c that were challenged with dengue virus serotype Den-1 Den-3. Second, compare the secretion of IL-10 of immune responses of Mice Balb/c that were challenged with dengue viruses in primary infection, secondary monospecific infection and secondary heterologous infection. This study was experimental laboratory, with randomised pretest-posttest with spread control group design. Balb/C mice were used as animal laboratory models. The dengue viruses Den-1 Den-3 that used in this study were isolated from dengue suspected patients and the viruses were innoculated and bred in BHK 21 clone 13 cell line, before they were injected to the models. Serum samples were collected from blood by centrifuging the blood for 10 minutes at 1000 rpm. The data of this experiment were obtained by applying the serum samples in Murine IL-10 Elisa Kit product of Diaclone Research France. In primary infection, significant increase of IL-10 mean titer in experimental group challenged with dengue viruses Den-1 was obtained (p=0.03) compared with the control group. Conversely the experimental group challenged with dengue viruses Den-3 showed no significant increase of mean titer (p=0.127). In the secondary infection, group treated with dengue Viruses Den-1 showed decrease of mean titer of IL-10 (p=0.034 p=0.003). Meanwhile, group treated with dengue Viruses Den-3 showed no significant different compared to primary infection group (p=0.571 p=0.359). The results of the study, support both virulence theory and the secondary heterologous dengue infection theory.
Research on the Selection of Vaccine Seeds with the HI Method Approach to Improve the Quality of Avian Infuenza Vaccines Nuraeni, Nining; Bustanudin, Entang; Susanti, Dine Resti; Rachmanita, Intan Nur; Wijayanti , Desri; A. Rantam, Fedik
Journal La Medihealtico Vol. 5 No. 2 (2024): Journal La Medihealtico
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallamedihealtico.v5i2.1305

Abstract

AI virus is a virus that is easily mutated because it has a single chain of RNA that is unstable, so many strains appear in the field. Currently in the field (in Indonesia), it is known that there are 2 vaccine strains that dominate, namely AI H5N1 Clade 2.1.3 and AI H5N1 Clade 2.3.2. The incompatibility of seed viruses used as vaccine seeds will hinder the eradication of AI disease in Indonesia. Therefore, it is necessary to select and develop seed viruses as new vaccine seed candidates to obtain vaccines with high quality, safe, homologous, and able to cover viruses in the field. Development activities carried out by researchers aim to obtain vaccine seeds that are able to protect against AI clade 2.1.3 virus and AI clade 2.3.2 virus. The vaccine candidates to be developed are several vaccine candidates derived from the isolation of chicken organs from AI outbreak areas in several areas on the island of Java which include West Java, Central Java and East Java which were found around 2010 to 2013. These isolates are processed and then propagated on the SPF TAB and passaged until a high viral titter is obtained. After that, identification, pathogenicity, immunogenicity and protective tests were carried out. From the results of this development, AI C-8 and C-11 virus seeds were obtained which are able to protect against both AI Clade 2.1.3 and AI Clade 2.3.2.
Co-Authors Bustanudin, Entang Iwang Yusuf Konthen, P.G. Nuraeni, Nining Rachmanita, Intan Nur Susanti, Dine Resti Wijayanti , Desri