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Pengaruh Penyuluhan terhadap Kepadatan Aedes aegypti dalam Pencegahan Demam Berdarah Zulaikhah, Siti Thomas; Yusuf, Iwang
Kes Mas: Jurnal Fakultas Kesehatan Masyarakat Vol 12, No 1 (2018): Kes Mas: Jurnal Fakultas Kesehatan Masyarakat
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.209 KB) | DOI: 10.12928/kesmas.v12i1.6627

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Background: Dengue infection is a public health problem and until now, the dengue eradication efforts have not been successfuly. In Indonesia, the incidence is still high and widely transmitted there fore the more intensive vector control is required. The purpose of this study was to verify that the counseling program about mosquitonest eradication effect the density of Aedes. Method: The research was quasi experiment using one group pre and post test design, interventions about counseling of mosquito to eradication. Samples were gathered from health center around Ngaliyan area which consisted of 6 districts. Each district was taken 100 houses by simple random sampling, so the total samples are 600 and each samples were testedor the presence of Aedes aegypti’s larvaes. Pretest was conducted on May 2013 and postest on August 2013 by conducting entomological survey to determine the density of Aedes aegypti’s larvae. Respondents are received 3 times counseling. Results: Data about Larva Free Index (ABJ), Container Index (CI), House Index (HI) and Breteau Index (BI) were tested for normality using Shapiro Wilk, and all were normally distributed (p>0.05), the data were analyzed using t-paired test. The result of Larva Free Index variable analysis obtained were p=0.011, CI p=0.000, HI p=0.035 and BI p=0.004. Conclusion: Counseling about mosquito eradication effected on Aedes aegypti’s larvae so it can prevent the outbreak of dengue fever.
Kualitas Spermatozoa Mencit Balb /C Jantan Setelah Pemberian Ekstrak Buah Kepel (Stelechocarpus Burahol) Fatmawati, Dina; Isradji, Israhnanto; Yusuf, Iwang; Suparmi, Suparmi
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (574.654 KB) | DOI: 10.15395/mkb.v48n3.845

Abstract

Buah kepel (Stelechocarpus burahol) terbukti memiliki kandungan fitoestrogen yang diduga memiliki pengaruh terhadap kualitas sperma, namun sejauh ini kajian ilmiah mengenai efek buah kepel terhadap kualitas sperma belum banyak dilakukan. Penelitian ini bertujuan mengetahui efek ekstrak buah kepel terhadap motilitas, konsentrasi, viabilitas, dan morfologi spermatozoa mencit jantan (Mus musculus). Penelitian ini merupakan penelitian eksperimental dengan rancangan post test only control group design. Ekstraksi buah kepel menggunakan metode sokletasi dengan pelarut etanol 96%. Sebanyak 24 ekor mencit yang dibagi ke dalam 4 kelompok, yaitu kelompok kontrol yang diberi akuades, kelompok I, II, dan III yang disonde ekstrak buah kepel (EBK) dengan dosis 0,65; 1,3; dan 2,6 mg/ekor maisng-masing selama 14 hari. Motilitas, konsentrasi, viabilitas, dan morfologi spermatozoa dianalisis dengan mengambil sampel sperma dari kauda epididimis pada hari ke-15. Berdasarkan hasil uji ANOVA menunjukkan bahwa viabilitas, morfologi, dan konsentrasi spermatozoa rata-rata antara kelompok kontrol dan perlakuan (I, II, III) tidak berbeda signifikan (p>0,05). Persentase motilitas spermatozoa mengalami penurunan secara signifikan pada kelompok III (2,6 mg/ekor) dibanding dengan kontrol, kelompok I, dan kelompok II (p<0,05). Simpulan yang dapat ditarik dari penelitian ini adalah ekstrak buah kepel dengan dosis 2,6 mg/ekor/hari dapat menurunkan kualitas spermatozoa sehingga berpotensi sebagai senyawa antifertilitas pada pria. [MKB. 2016;48(3):155–59]Kata kunci: Kepel (Stelechocarpus buharol), konsentrasi spermatozoa, kualitas spermatozoa, Sperm Quality of Male Balb/C Mice after Kepel (Stelechocarpus burahol) Fruit Extract AdministrationKepel (Stelechocarpus burahol) fruits contains phytoestrogen that have been shown to have a potential phytoestrogen that may affect sperm quality. However, few studies on this effect have been conducted. The aim of this study was to evaluate the effect Stelechocarpus burahol administration on sperm quality in mice. This experimental study used post-test only with control-group design. The sox-chlelation method was performed to obtain the ethanol extract from kepel fruit. Twenty four mice were randomly divided into 4 groups of kepel fruit extract dose: 0 mg/mice (control or group I), 0.65 mg/mice (group II), 1.3 mg/mice (group III), and 2.6 mg/mice (group IV).  One milliliter of kepel fruit extract was administered orally every day for 14 days. On day 15 the sperm were collected and analyzed for motility, concentration, viability, and morphology. There was no effect of kepel fruit extract found among the treated groups (group I, II, III, IV) on the percentage of sperm concentration, morphology, and viability (p>0.05). However, the percentage of motility in group IV (kepel fruit extract at a dose of 2.6 mg/mice) was decreased significantly compared to control and other treatment groups (p<0.05). In conclusion, 2.6 mg/mice Kepel extract  decreases sperm quality; hence, it is a potential candidate for antifertility in men. [MKB. 2016;48(3):155–9]Key words: Kepel (Stelechocarpus burahol), sperm concentration, sperm quality
DETEKSI INTERLEUKIN-10 (IL-10) PADA MICE BALB/C YANG DIINFEKSI DENGAN VIRUS DENGUE: TELAAH PATOGENESIS ANTARA TEORI INFEKSI SEKUNDER DAN VIRULENSI Yusuf, Iwang; Konthen, P.G.; A. Rantam, Fedik
Jurnal Kedokteran YARSI Vol 13, No 1 (2005): JANUARI - APRIL 2005
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.106 KB) | DOI: 10.33476/jky.v13i1.1053

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Since the pathogenesis/immunopathogenesis of vasculopathy plasma leakage in DHF/ DSS was not clearly understood, this study was conducted. Considering the equilibrium between pro and anti inflammatory cytokine in homeostatic process in inflammation, this study was aimed to; first, detect the increase of secretion of IL-10 of immune responses of Mice Balb/c that were challenged with dengue virus serotype Den-1 Den-3. Second, compare the secretion of IL-10 of immune responses of Mice Balb/c that were challenged with dengue viruses in primary infection, secondary monospecific infection and secondary heterologous infection. This study was experimental laboratory, with randomised pretest-posttest with spread control group design. Balb/C mice were used as animal laboratory models. The dengue viruses Den-1 Den-3 that used in this study were isolated from dengue suspected patients and the viruses were innoculated and bred in BHK 21 clone 13 cell line, before they were injected to the models. Serum samples were collected from blood by centrifuging the blood for 10 minutes at 1000 rpm. The data of this experiment were obtained by applying the serum samples in Murine IL-10 Elisa Kit product of Diaclone Research France. In primary infection, significant increase of IL-10 mean titer in experimental group challenged with dengue viruses Den-1 was obtained (p=0.03) compared with the control group. Conversely the experimental group challenged with dengue viruses Den-3 showed no significant increase of mean titer (p=0.127). In the secondary infection, group treated with dengue Viruses Den-1 showed decrease of mean titer of IL-10 (p=0.034 p=0.003). Meanwhile, group treated with dengue Viruses Den-3 showed no significant different compared to primary infection group (p=0.571 p=0.359). The results of the study, support both virulence theory and the secondary heterologous dengue infection theory.
Pengaruh Lendir Bekicot (Achatina fulica) terhadap Jumlah Sel Fibroblas pada Penyembuhan Luka Sayat Studi Eksperimental pada Kulit Mencit (Mus musculus) Purnasari, Perez Wahyu; Fatmawati, Dina; Yusuf, Iwang
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 4, No 2 (2012): Juli-Desember 2012
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.648 KB) | DOI: 10.30659/sainsmed.v4i2.376

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Background: Wound healing is very important to return skin as soon as possible and the integration is a complex and dynamic process. Proliferation phase involving fibroblasts is one of important step-on wound healing. Use of drugs such as povidone iodine on cuts aims to accelerate wound healing is still being debated. Healing by land snails’ mucus can be an alternative because it has many benefits. This study wants to know about the influence of land snails’ mucus on the number of fibroblasts in healing cuts.Design and methods: The study is an experimental research with post test only randomized control group design. The subjects were 18 Mus musculus strain mice that were divided into 3 groups. 1st group as a control group of untreated, 2nd group was given povidone iodine 10%, and 3rd group was given land snails’ mucus during 5th days. On the 6th day the mices were killed for histological study.Results: The data showed that average number of fibroblast in 1st group is 93,85; 2nd group is 126,37; and 3rd is group 217,5. The results of one way anova statistical test showed significant difference among the three groups p = 0,000. The results of post hoc test showed significant difference in all groups (p < 0,05).Conclusion: The mucus of land snail is influence the number of fibroblast in healing cuts (Sains Medika, 4(2):195-203).
Cytotoxic Effect of Ethanolic Extract of Sarang Semut (Myrmecodia pendens) on HeLa Cervix Cancer Cell Line In Vitro Experimental Study Fatmawati, Dina; Puspitasari, Prista Karina; Yusuf, Iwang
Sains Medika : Jurnal Kedokteran dan Kesehatan Vol 3, No 2 (2011): Juli-Desember 2011
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.338 KB) | DOI: 10.30659/sainsmed.v3i2.393

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Background: Sarang semut (Myrmecodia pendens) is a traditional plant that has been known to contain anti cancer components. Flavonoids and tannins were contained in sarang semut plant which are believed has cytotoxic effect against cancer cell line. This study aims at cytotoxic effect ethanolic extract of sarang semut at various concentrations on HeLa cervical cancer cell line.Design and Method: The method was quasi experimental with post test only non equivalent control group design. HeLa cell was divided into two groups. The first group as positive control with doxorubicin, second group as treatment with ethanolic extract of sarang semut at various concentrations. Ethanolic extract of sarang semut concentrations used were 3,91 μg/ml; 7,81 μg/ml; 15,63 μg/ml; 31,25 μg/ml; 62,50 μg/ml; 125 μg/ml; 250 μg/ml; 500 μg/ml; 1000 μg/ml. Cytotoxic effect was evaluated by direct counting method with tryphan blue dye then using probit regression analysis to find IC50 value.Result: Inhibitory concentration 50 (IC50) value ethanol extract of sarang semut was 33,28 μg/ml. Ethanol extract of sarang semut had a cytotoxicity effect categorized as the moderately active (20 ìg/ml< IC50< 100ìg/ ml). Inhibitory concentration 50 (IC50) value doxorubicin was 5,56 μg/ml. Cytotoxicity effect of doxorubisin higher than cytotoxicity effect of ethanolic extract of sarang semut.Conclusion: Ethanolic extract of sarang semut (Myrmecodia pendens) had a cytotoxic effect categorized as the moderately active on HeLa cell (Sains Medika, 3(2):112-120).
Phagocytic Index of Peritoneal Macrophages after Propolis Suplementation in Mice (Mus musculus) Mustafiah, Siti Eva; Fatmawati, Dina; Yusuf, Iwang
Sains Medika : Jurnal Kedokteran dan Kesehatan Vol 3, No 2 (2011): Juli-Desember 2011
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.499 KB) | DOI: 10.30659/sainsmed.v3i2.394

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Background: Various diseases depend on individual’s immunity. Many of which nowadays have not had the effective and formal therapy that makes experts researches the best and the most effective way for it; the use of Propolis as immunomodulator is one of them. This research aims for knowing the effect of propolis towards peritoneal macrophage phagocytic index on mice.Design and Method: This research is an experiment with post test study design randomized control group design. This study used mice that were divided into four groups randomly. The first group / Group-I were for negative control (standard feed and aquadest); The second group/Group-II were fed standard-feed, aquadest, and propolis at a dose of 1.25 mg/kgBM; the third group/group-III were fed standard-feed, water, and propolis at a dose of 2.5 mg/KgBM; The fourth group/Group-IV were fed standard feed, water, and propolis at a dose of 5 mg/KgBM. Treatment where conducted for 3 days.Result: The average macrophage phagocytic index, were at the highest level of it (7.82 1.63) while the lowest one were the first group 3.43 0.13. The Kruskall Wallis result stated that there is index difference among various groups with p 0.002 (p < 0.05).Conclusion: Propolis effected on mice peritoneal macrophage phagocytosis index (Sains Medika, 3(2):121-128).
Pemeriksaan Kesehatan dan Edukasi tentang Latihan Sendi Bahu untuk Mengatasi Nyeri bagi Warga Getasan Kabupaten Semarang Rosdiana, Ika; Masfiyah, Masfiyah; Listiarini, Dian Ayu; Santosa, Wignyo; Yusuf, Iwang; Suparmi, Suparmi
Jurnal Pengabdian Masyarakat Kedokteran Vol 3, No 2 (2024): Mei
Publisher : Fakultas Kedokteran, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/abdimasku.3.2.39-46

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Nyeri bahu merupakan nyeri yang sering terjadi seiring dengan bertambahnya usia. Nyeri tersebut membatasi kemampuan untuk mengangkat lengan di atas kepala atau memutar bahu. Faktor risiko nyeri bahu dilaporkan berhubungan dengan berbagai faktor sosio-demografis, klinis, pekerjaan, dan berbagai paparan fisik dalam pekerjaan. Mata pencaharian penduduk yang sebagian besar bekerja sebagai petani dan bercocok tanam di kebun, serta kondisi geografis yang naik turun memungkinkan seseorang harus berjalan dengan tenaga lebih dibandingkan dengan yang tinggal di perkotaan sehingga berpotensi untuk mengalami nyeri pada sendi sendi. Tujuan kegiatan pengabdian kepada masyarakat (PkM) ini adalah untuk memberikan pemeriksaan kesehatan dan edukasi latihan sendi bahu untuk mengurangi nyeri bagi masyarakat Dusun Mulungan. Selain itu, jika dibutuhkan pasien diberikan pengobatan gratis sesuai dengan keluhan yang dialami berdasarkan hasil anamnesa dokter yang terlibat. Metode pelaksanaan PkM terdiri dari 2 tahap: (1) penyuluhan kesehatan mengenai penyakit nyeri bahu dan tatalaksana latihan sederhana, (2) pemeriksaan dan pengobatan gratis. Sebagian besar masyarakat yang memeriksaan kesehatan adalah perempuan 44(77,2%), dan usia lanjut 25 (43,9%). PkM di dusun Mulungan, Getasan, Kabupaten Semarang bermanfaat sebagai upaya peningkatan kesehatan masyarakat yang memiliki keterbatasan akses layanan kesehatan.A frozen shoulder is a pain that often occurs with age. The pain limits the ability to raise the arm above the head or rotate the shoulder. Risk factors for shoulder pain are reported to be related to various factors, including socio-demographic, clinical, and occupational, as well as different occupational physical exposures. The livelihoods of the population, most of whom work as farmers and grow crops in gardens, as well as the ups and downs of geographical conditions, mean that people have to walk with more energy than those who live in urban areas, so they have the potential to experience joint pain. This community service activity (PkM) aims to provide health checks and education on shoulder joint exercises to reduce pain for the people of Mulungan Hamlet. Apart from that, if necessary, patients are given free treatment according to the complaints they are experiencing based on the results of the anamnesis of the doctors involved. The PkM implementation method consists of 2 stages: (1) health education on shoulder pain and simple exercise management, and the second stage is free examination and treatment. Most who had health checks were women 44 (77.2%), and 25 (43.9%) elderly. PkM in Dusun Mulungan, Getasan, Semarang Regency, helps improve people's health with limited access to health services.
PENGARUH EKSTRAK KAYU MANIS (CINNAMOMUM BURMANNII) TERHADAP INDEKS ULKUS GASTER STUDI EKSPERIMENTAL PADA TIKUS JANTAN GALUR WISTAR YANG DIINDUKSI IBUPROFEN Ramadhani, Syifa Eka; Cahyono, Erwin Budi; Yusuf, Iwang; ., Sumarno
Jurnal Ilmiah Penelitian Mahasiswa Vol 3, No 4 (2024): Desember 2024
Publisher : Jurnal Ilmiah Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kayu manis merupakan salah satu tanaman tropis yang memiliki banyak spesies utama, salah satunya yaitu Cinnamomum burmannii. Kayu manis memiliki kandungan senyawa utama yaitu synalmadehide, flavonoid, dan tannin. Senyawa tersebut berfungsi sebagai antiinflamasi, analgesic, antioksidan, antibakteri, dan menurunkan aktivitas radikal bebas. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kayu manis terhadap indeks ulkus gaster.Jenis penelitian ini adalah eksperimental dengan desain post-test randomized controlled group. Subjek uji yang digunakan adalah tikus putih jantan galur wistar yang dihitung jumlahnya menggunakan rumus Federer. Sampel pada penelitian ini berjumlah 28 tikus yang telah memenuhi kriteria inklusi dan eksklusi. Penelitian ini menggunakan kulit batang kayu manis yang telah diekstraksi dengan metode infundasi. Penilaian pada subjek uji menggunakan tabel indeks ulkus gaster untuk menilai adanya jumlah dan keparahan tukak. Analisis statistik menggunakan uji deskriptif yang kemudian diolah menggunakan uji normalitas Saphiro-Wilk dan uji homogenitas Levene”s Test yang kemudian dilanjutkan uji non parametrik Kruskal-Walls dan uji signifikansi menggunakan post hoc Mann-Whitney dengan program SPSS.Hasil uji non parametrik menggunakan uji analisis Kruskal-Walls didapatkan nilai p value = 0,021 (p < 0,05) sehingga dapat disimpulkan bahwa terdapat perbedaan rerata skor indeks ulkus gaster pada seluruh kelompok. Hasil uji analisis menggunakan uji post hoc Mann-Whitney untuk mengetahui perbedaan rerata skor indeks ulkus gaster antar kelompok kontrol dan percobaan didapatkan nilai p value = 0,060 (p < 0,05). Dari hasil tersebut dapat disimpulkan bahwa pemberian ekstrak kayu manis dengan metode infundasi memiliki pengaruh yang tidak signifikan terhadap skor indeks ulkus gaster.Kata Kunci: ekstrak kayu manis, antiinflamasi, flavonoid, ulkus gaster
How Female Adolescents Exposed To Pornography? Sari, Rita Kartika; Anggara, Andhika Dwi; Intan, Yulice Soraya Nur; Mayangsari, Citra Primavita; Yusuf, Iwang; Trisnadi, Setyo; Rinawati
Public Health of Indonesia Vol. 11 No. 3 (2025): July - September
Publisher : YCAB Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36685/phi.v11i3.1008

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Background: The digital era has markedly increased adolescents’ exposure to explicit content, including pornography, raising significant concerns regarding its impact on sexual and reproductive health. This is particularly critical among female adolescents in Indonesia, where access to comprehensive sexual education remains limited. Objective: This study aims to examine the exposure of female adolescents to pornographic content, with a focus on patterns of media consumption and their implications for reproductive health. Methods: A descriptive cross-sectional study was conducted among 58 female adolescents aged 13 to 18 years in Karanganyar, Indonesia. Data were collected using a validated questionnaire and analyzed through descriptive statistical methods to identify patterns of media usage and exposure to pornography. Results: The findings indicated that the internet and handheld devices were the predominant sources of media consumption (96.6%), significantly contributing to exposure to pornographic material (86.2% and 96.6%, respectively). Television and print media also played contributory roles, particularly through soap operas and Western films. Exposure to pornography was associated with limited awareness of reproductive health, especially among younger participants. The exposure was further linked to distorted perceptions of sexual behavior, negative body image, and potential psychological consequences such as anxiety and low self-esteem. Conclusion: This study highlights the urgent need for targeted interventions to enhance critical media literacy and provide comprehensive sexual education for adolescents. Addressing these issues is essential to mitigate the adverse effects of pornography exposure on reproductive health, while taking into account cultural and contextual factors. Future research should investigate the longitudinal effects and gender-specific outcomes to inform the development of tailored preventive strategies. Keywords: adolescents; pornography; media exposure; reproductive health; sexual education
HUBUNGAN DURASI ANESTESI UMUM DENGAN TINGKATAN NYERI TENGGOROKAN PASCA ANESTESI PENELITIAN OBSERVASIONAL PADA PASIEN DI RUMAH SAKIT ISLAM SULTAN AGUNG SEMARANG Maulana, Lalu Arya Sahasika; Listiarini, Dian Ayu; Yusuf, Iwang
Jurnal Ilmiah Penelitian Mahasiswa Vol 4, No 3 (2025): SEPTEMBER 2025
Publisher : Jurnal Ilmiah Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar Belakang: Nyeri tenggorokan pasca anestesi umum adalah komplikasi umum yang dapat memengaruhi kenyamanan pasien dan memperlambat pemulihan. Penelitian menunjukkan bahwa durasi anestesi yang lebih lama berhubungan dengan peningkatan insiden nyeri tenggorokan. Oleh karena itu, penelitian ini bertujuan untuk mengeksplorasi hubungan antara durasi anestesi umum dan tingkat nyeri tenggorokan pada pasien di Rumah Sakit Islam Sultan Agung Semarang. Metode: Studi observasional dengan desain cross-sectional dilakukan terhadap 35 pasien yang menjalani anestesi umum dengan intubasi. Durasi anestesi dikategorikan menjadi kurang dari 2 jam dan 2 jam atau lebih. Tingkat nyeri tenggorokan diukur menggunakan Visual Analogue Scale (VAS). Analisis bivariat menggunakan uji Chi-square dilakukan untuk mengidentifikasi hubungan antara durasi anestesi dan tingkat nyeri tenggorokan. Hasil: Hasil menunjukkan bahwa 40% pasien mengalami nyeri ringan, 34,3% tidak mengalami nyeri, 13,1% mengalami nyeri sedang, dan 8,6% mengalami nyeri berat. Analisis bivariat mengungkapkan hubungan signifikan antara durasi anestesi dan tingkat nyeri tenggorokan (p = 0,032). Kelompok dengan durasi anestesi ≥ 2 jam memiliki proporsi lebih tinggi untuk nyeri sedang dan berat.. Kesimpulan: Durasi anestesi umum yang lebih lama berkorelasi signifikan dengan peningkatan tingkat nyeri tenggorokan pasca anestesi. Temuan ini menekankan pentingnya pengelolaan durasi anestesi untuk meminimalkan komplikasi pascaoperasi. Kata Kunci: anestesi umum, nyeri tenggorokan, durasi anestesi, VAS