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All Journal JURNAL BUDIDAYA PERTANIAN Biofaal Journal Media Pertanian Tropical Small Island Agriculture Management Rumphius Pattimura Biological Journal
Patty, Jogeneis
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Keanekaragaman Jamur Makroskopis Di Kecamatan Amahai Kabupaten Maluku Tengah Uspitany, Lucas; Patty, Jogeneis; Uruilal, Costanza
Tropical Small Island Agriculture Management Vol 4 No 2 (2024): Tropical Small Island Agriculture Management
Publisher : Pattimura University Ambon Maluku Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/tsiam.2024.4.2.58

Abstract

The research objectives are: 1) Identifying the types of macroscopic fungi found in Amahai District; 2) Calculate the frequency of presence and population of macroscopic fungi found in Amahai District, and 3) Describe the morphological characteristics of macroscopic fungi found in the Amahai sub-district. This research was carried out in Amahai District in the villages of Yafila and Nuweletetu. This research was also carried out at the Plant Disease Laboratory, Faculty of Agriculture, Pattimura University, from March to May 2024. The survey was carried out using the roaming method to obtain macroscopic fungi in Amahai District. The transect line created as a sampling location measures 100 ´ 20 m. Laboratory identification is carried out to determine the morphological characteristics of the fungus. Mushroom characteristics include fruit body shape, color, size, and habitat. The results (PCA) of 13 morphological characters from 38 fungi samples showed that between the two groups of macroscopic fungi, there was a similarity in morphological characters of 62%. Principle component analysis (PCA-Biplot) was performed on nine morphological characters on 38 macroscopic fungi samples. PCA was carried out to obtain characteristic characters in the fungi groups found. Based on the results of the analysis (PCA), the fruit's body shape and pores can be recommended as characterizing characteristics.
IN VITRO ANTAGONISM OF THE ISOLATE MUSHROOM Trichoderma harzianum TO Fusarium oxyzporum f. sp. Cubense CAUSES WILT DISEASE IN BANANA KEPOK (Musa paradisiaca normalis) Pattikawa, Apriyanti Santi; Pagaya, Joseph; Patty, Jogeneis
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p050-055

Abstract

Trichorderma harzianum is a soil native fungus that is beneficial because it has high antagonistic properties against plant pathogenic fungi. Control mechanisms can be: antibiosis, parasitism, competition and hyphae interference. T. harzianum as a biological control agent for the pathogenic fungus Fusarium oxyforum f. sp. Cubense is an alternative to control this pathogenic fungus, without causing a negative impact on the environment. The aim of the study was to determine whether Trichodernma harzianum isolates Piru, Kairatu and Uraur could suppress the growth of the fungus Fusarium oxyforum f. sp. cubense and Trichorderma harzianum which isolates (Piru isolate, Kairatu isolate and Uraur isolate) had faster inhibition against Fusarium oxyforum f. sp. cubense causes wilt disease on banana kepok (Musa paradisiaca normalis) in vitro with antagonism test. The research was conducted in 2 places, namely in the HPT BPTPH laboratory in Maluku province and continued in the microbiology laboratory at FMIPA Unpatti. The research method used was laboratory experimental in nature by using a completely randomized design with factorial pattern with three treatments and three replications. The results showed that the cause of wilt disease in banana plants was Fusarium oxyforum f. sp. Cubense. Antagonism test in vitro showed that T. harzianum Piru isolate showed the highest inhibition zone (70.87%), both Kairatu isolates (45.27%), and the lowest by Uraur isolate (32.61%) so that it can be said that T. harzianum has the potential to inhibit the growth of Fusarium fungus. oxyforum f. sp. Cubense.
Antagonisme In-Vitro Lima Isolat Lokal Trichoderma spp. Asal Rhizosfer Terhadap Rhizoctonia solani Penyebab Busuk Pelepah Jagung Soplanit, Rainhart C; Patty, Jogeneis; Talahaturuson, Abraham
JURNAL BUDIDAYA PERTANIAN Vol 17 No 2 (2021): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp/2021.17.2.89

Abstract

Environmentally friendly biological control can be an alternative for suppressing the pathogen Rhizoctonia solani, the cause of sheath rot in corn plants. This can be done by utilizing biological agents, such as Trichoderma spp. Research on in vitro antagonism of five local isolates of Trichoderma spp. of rhizospheric origin against R. solani was carried out at the Plant Disease Diagnosis Laboratory, Faculty of Agriculture Unpatti, Ambon. This research aimed to determine the inhibition of five Trichoderma spp isolates against R. solani in vitro and to the mechanism of its inhibition. This study used a Completely Randomized Design to test the five local isolates of Trichoderma spp. The experiment was carried out with 4 replications, and each experimental unit consisted of 2 Petri dishes for testing. The results showed that the five local isolates of Trichoderma spp. from the rhizosphere could suppress the growth of R. solani on corn plants in vitro. On the seventh day of observation, the highest inhibition occurred in Trichoderma isolate from the Banda Baru coconut rhizosphere (TrichoRKlBB), which was 100% and the local Trichoderma isolate rhizosphere of Nuruwe cocoa (TrichoRKN) 100%, and the lowest percentage occurred using the Trichoderma isolate rhizosphere of Waisamu coconut (TrichoRKlW), namely (80.13%). The mechanisms of antagonism that occured were the mechanisms of competition and microparasitism.
AGIHAN PENYAKIT PADA TANAMAN JERUK MANIS KISAR (Citrus spp.) DI PULAU KISAR, KABUPATEN MALUKU BARAT DAYA Patty, Jogeneis; Leiwakabessy, Ch.; Uruilal, Costanza; Rumahlewang, Wilhelmina
Biofaal Journal Vol 3 No 2 (2022): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v3i2pp96-104

Abstract

Jeruk Kisar merupakan komoditas buah andalan di pulau Kisar. Penelitian ini bertujuan untuk mengetahui jenis-jenis penyakit pada tanaman jeruk manis Kisar (Citus spp), intensitas penyakit dan kategori serangannya, serta sebaran sebaran jenis penyakit jeruk manis Kisar di Pulau Kisar Kabupaten Maluku Barat Daya. Penelitian ini merupakan penelitian deskriptif dengan menggunakan metode survey dan penentuan tanaman sampel secara acak sederhana (Simple random sampling). Jenis-jenis penyakit dan intensitas kerusakan serta kategori serangannya pada tanaman jeruk manis Kisar adalah CVPD (Liberobacter asiaticum) 8.58% (ringan), kulit Diplodia (Botrydiplodia theobomae) 39.67% (sedang), kanker jeruk (Xanthomonas axonopodis pv. citri), 46.39% (sedang), dan jamur upas (Corticium salmonicolor) 43.78% (sedang). Kondisi areal yang mempengaruhi terjadinya penyakit adalah teknik budidaya yang menyimpang yang menunjang perkembangan penyakit adalah tindakan pemeliharaan yang kurang baik, tidak dilakukan sanitasi terhadap bagian tanaman yang sakit sehingga sumber inokulum selalu ada, dan tidak pernah dilakukan pengendalian penyakit dengan pestisida.
Pengaruh Konsentrasi dan Frekuensi POC Biofouling Tiram Mutiara terhadap Kerapatan Stomata Hotong (Setaria italica (L.) Nendissa, Jeanne Ivonne; Riry, Johan; Patty, Jogeneis; Amba, Martha; Wahditiya, Andi Adriani; Lating, Raudatul Janna
Media Pertanian Vol 10, No 2 (2025): Media Pertanian
Publisher : Program Studi Agroteknologi Universitas Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37058/mp.v10i2.16433

Abstract

Ketersediaan nutrisi berperan penting dalam mengatur fungsi fisiologis tanaman, termasuk pembentukan dan kerapatan stomata yang memengaruhi efisiensi fotosintesis serta penggunaan air. Salah satu sumber nutrisi organik potensial adalah Pupuk Organik Cair (POC) berbasis biofouling Tiram Mutiara, yang mengandung unsur hara esensial seperti nitrogen, fosfor, dan kalium. Penelitian ini bertujuan untuk menganalisis pengaruh konsentrasi dan frekuensi aplikasi POC biofouling Tiram Mutiara terhadap kerapatan stomata tanaman hotong (Setaria italica (L.)). Penelitian dilaksanakan di Desa Mamala, Kabupaten Maluku Tengah, dari Oktober 2023 hingga Januari 2024, menggunakan rancangan acak kelompok lengkap (RAKL) dua faktor, yaitu konsentrasi POC (0, 10, 20, dan 30 mL/L) serta frekuensi aplikasi (satu kali pada 25 Hari Setelah Tanam [HST] dan dua kali pada 25 dan 50 HST). Hasil analisis varians menunjukkan bahwa kedua faktor berpengaruh signifikan terhadap kerapatan stomata, tinggi tanaman, dan jumlah daun. Perlakuan P3W2 (30 mL/L, dua kali aplikasi) memberikan nilai tertinggi dengan kerapatan stomata 366,67 stomata/cm². Hasil ini menunjukkan bahwa peningkatan konsentrasi dan frekuensi aplikasi POC biofouling meningkatkan aktivitas fisiologis tanaman melalui pembentukan stomata yang lebih padat dan efisien. Dengan demikian, POC biofouling Tiram Mutiara berpotensi digunakan sebagai pupuk organik ramah lingkungan yang mendukung pertumbuhan dan efisiensi fotosintesis tanaman hotong.  Nutrient availability plays a crucial role in regulating plant physiological functions, including stomatal formation and density, which directly affect photosynthetic efficiency and water use. One promising organic nutrient source is Pearl Oyster biofouling-based liquid organic fertilizer (POC), which contains essential elements such as nitrogen, phosphorus, and potassium. This study aimed to examine the effects of different concentrations and application frequencies of Pearl Oyster biofouling POC on the stomatal density of foxtail millet (Setaria italica (L.)). The research was conducted in Mamala Village, Central Maluku Regency, from October 2023 to January 2024, using a randomized complete block design (RCBD) with two factors: POC concentration (0, 10, 20, and 30 mL/L) and application frequency (once at 25 Days After Planting [DAP] and twice at 25 and 50 DAP). Analysis of variance revealed significant effects of both factors on stomatal density, plant height, and leaf number. The highest stomatal density (366.67 stomata/cm²) was observed in treatment P3W2 (30 mL/L applied twice). These findings indicate that higher concentrations and more frequent applications of biofouling-based POC enhance plant physiological activity by promoting denser and more efficient stomatal development. Therefore, Pearl Oyster biofouling POC can serve as an environmentally friendly organic fertilizer that supports improved growth and photosynthetic efficiency in Setaria italica (L.).
Co-Authors Abraham Talahaturuson, Abraham Amba, Martha Andi Adriani Wahditiya Lating, Raudatul Janna Leiwakabessy, Christoffol Nendissa, Jeanne Ivonne Pagaya, Joseph Pattikawa, Apriyanti Santi Soplanit, Rainhart C Uruilal, Costanza Uspitany, Lucas Wilhelmina Rumahlewang