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All Journal Tropical Small Island Agriculture Management Rumphius Pattimura Biological Journal
Patty, Jogeneis
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Keanekaragaman Jamur Makroskopis Di Kecamatan Amahai Kabupaten Maluku Tengah Uspitany, Lucas; Patty, Jogeneis; Uruilal, Costanza
Tropical Small Island Agriculture Management Vol 4 No 2 (2024): Tropical Small Island Agriculture Management
Publisher : Pattimura University Ambon Maluku Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/tsiam.2024.4.2.58

Abstract

The research objectives are: 1) Identifying the types of macroscopic fungi found in Amahai District; 2) Calculate the frequency of presence and population of macroscopic fungi found in Amahai District, and 3) Describe the morphological characteristics of macroscopic fungi found in the Amahai sub-district. This research was carried out in Amahai District in the villages of Yafila and Nuweletetu. This research was also carried out at the Plant Disease Laboratory, Faculty of Agriculture, Pattimura University, from March to May 2024. The survey was carried out using the roaming method to obtain macroscopic fungi in Amahai District. The transect line created as a sampling location measures 100 ยด 20 m. Laboratory identification is carried out to determine the morphological characteristics of the fungus. Mushroom characteristics include fruit body shape, color, size, and habitat. The results (PCA) of 13 morphological characters from 38 fungi samples showed that between the two groups of macroscopic fungi, there was a similarity in morphological characters of 62%. Principle component analysis (PCA-Biplot) was performed on nine morphological characters on 38 macroscopic fungi samples. PCA was carried out to obtain characteristic characters in the fungi groups found. Based on the results of the analysis (PCA), the fruit's body shape and pores can be recommended as characterizing characteristics.
IN VITRO ANTAGONISM OF THE ISOLATE MUSHROOM Trichoderma harzianum TO Fusarium oxyzporum f. sp. Cubense CAUSES WILT DISEASE IN BANANA KEPOK (Musa paradisiaca normalis) Pattikawa, Apriyanti Santi; Pagaya, Joseph; Patty, Jogeneis
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p050-055

Abstract

Trichorderma harzianum is a soil native fungus that is beneficial because it has high antagonistic properties against plant pathogenic fungi. Control mechanisms can be: antibiosis, parasitism, competition and hyphae interference. T. harzianum as a biological control agent for the pathogenic fungus Fusarium oxyforum f. sp. Cubense is an alternative to control this pathogenic fungus, without causing a negative impact on the environment. The aim of the study was to determine whether Trichodernma harzianum isolates Piru, Kairatu and Uraur could suppress the growth of the fungus Fusarium oxyforum f. sp. cubense and Trichorderma harzianum which isolates (Piru isolate, Kairatu isolate and Uraur isolate) had faster inhibition against Fusarium oxyforum f. sp. cubense causes wilt disease on banana kepok (Musa paradisiaca normalis) in vitro with antagonism test. The research was conducted in 2 places, namely in the HPT BPTPH laboratory in Maluku province and continued in the microbiology laboratory at FMIPA Unpatti. The research method used was laboratory experimental in nature by using a completely randomized design with factorial pattern with three treatments and three replications. The results showed that the cause of wilt disease in banana plants was Fusarium oxyforum f. sp. Cubense. Antagonism test in vitro showed that T. harzianum Piru isolate showed the highest inhibition zone (70.87%), both Kairatu isolates (45.27%), and the lowest by Uraur isolate (32.61%) so that it can be said that T. harzianum has the potential to inhibit the growth of Fusarium fungus. oxyforum f. sp. Cubense.
Co-Authors Pagaya, Joseph Pattikawa, Apriyanti Santi Uruilal, Costanza Uspitany, Lucas