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Djaenuddin, Nurasiah

Balai Penelitian Tanaman Serealia

Author-ID : 1547699

Agriculture, Biological Sciences & Forestry

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Viabilitas kombinasi bakteri dan cendawan pada berbagai komposisi bahan pembawa dan efektifitasnya dalam mendekomposisi biomas jagung Djaenuddin, Nurasiah; Faesal, Faesal; Syafruddin, Syafruddin
Jurnal Penelitian Pertanian Tanaman Pangan Vol 2, No 2 (2018): Agustus 2018
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

One alternative to accelerate the decomposition of lignin or cellulose organic matter is to use microorganisms. BacterialÂ combined with fungi and synergistic will produce good compost. To ensure the viability and effectiveness of the decomposer microorganisms a suitable carrier material is required. The objective of the study were to obtain a combination of microorganisms isolates (bacteria and fungi) in accordance with the formulation of the carrier in supporting their viability and effectiveness to decomposting of maize biomass. The results showed that the combination of isolatesÂ E7.7 + P7 and B7.1 + O5 up to 28 weeks' storage were relatively superior than Promi and EM4. At 28 weeks storage,Â population of bacterial E7.7+P7Â is 2,1-2,75x109 cfu/g and fungusÂ 0,3-0,48x109 cfu/g, isolate B7.1+O5 had bacterialÂ Â 1,38-2,5x109 cfu/g and fungusÂ 0,2-0,3x109 cfu/g. While Promi and EM4 had bacterial were about 0.1-1.5x109 cfu/g and fungus 0.1x109 cfu/g. Both of these isolateÂ Â to decomposition of maize biomass had lower C/N and water content, and higher P and K content than EM4. Therefore, both of the combination of bacteria and fungi (E7.7 + P7 and B7.1 + O5) are worthy to biodecomposer for the making of compost fertilizer. To maintain viability and effectiveness of microbe E7.7+P7 was a better to use sago+compost, while for the combination of B7.1+O5 can using sago+compost and alternative is rice water+zeolit carrier material. The results of identification based on 16S rRNA sequence showed that isolates B7.1 and E7.7, respectively were Bacillus cereus strain ATCC14579 and Brevundimonas diminuta strain NBRC12967, while isolates of fungus O5 and P7 respectively were are Aspergillus fumigatus strains KARVSO4, and Aspergillus fumigatus strains DAOM-21534-28S.

Identifikasi dan Efektivitas Bakteri Dekomposer Terhadap Limbah Batang daun Jagung untuk Pupuk Organik Faesal, Faesal; Djaenuddin, Nurasiah; Soenartiningsih, Soenartiningsih
Jurnal Penelitian Pertanian Tanaman Pangan Vol 1, No 2 (2017): Agustus 2017
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Maize waste is difficult to decompose naturally, therefore needed special treatment to accelerate dissolved of maize waste. Identification and effectivity tested of decomposer bacteria on maize waste for organic fertilizer is one ways to overcome these problem. The research aim to find effective bacteria as decomposer of maize waste as organic fertilizer. Research was conducted in South Sulawesi Indonesia in June to Desember 2013. The first step in this research was collecting soil sample from many natural habitat ie: maize planting site, heap of stem and corn cob, heap of paddy straw or husk, and heap of sorghum stem. The second step where soil sample bring to the Pest and Plant Diseases Laboratory of ICERI for identify decomposer cellulolitic bacteria. We have identified 120 isolate than screened by temperature 50o C and we found of 29 decomposer bacteria by assumption. Furthermore those isolate were tested in vitro 29 sellulolitic bacteria and we found 16 effective sellulolitic bacteria. Than 16 effective sellulolitic bacteria we tested by using stem and leaf of maize in green house and finally found of 6 effective decomposer bacteria, which more effective compared to EM4 as a control. Those isolate decomposer bacteria more effective are: E7.1, E7.3, B7.1 (Bacillus sp), E7.7, H7.1 (Escherichia sp), and C4.1 (Micrococcus sp).

Kombinasi Biopestisida Formulasi Bacillus subtilis BNt8 dan Pestisida Nabati untuk Pengendalian Penyakit Hawar Daun Bipolaris maydis pada Jagung Djaenuddin, Nurasiah; Suriani, Suriani; Talanca, Andi Haris
Jurnal Penelitian Pertanian Tanaman Pangan Vol 2, No 1 (2018): April 2018
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Maydis leaf blight disease caused by Bipolaris maydis can attack corn plants in the vegetative and generative phase and can reduce yield up to 70%. Biological control of plant diseases with the use of biopesticides alone or in combination with other botanical materials can be efficient because it is easy to obtain, cheaper price, also environmentally friendly. The objective of the study was to obtain a combination of B. subtilis formulation with a botanical pesticide which has the potential to control the growth and development of maydis leaf blight. In addition to this, we also observed the effect of the application of the combination of botanical pesticides and B. subtilis formulation on plant growth. The study was conducted on February till June 2016 at the Plant Pathology Laboratory of ICERI with a complete randomized design and the Bajeng Experimental Farm using a randomized block design. In vitro testing, the treatment with vegetable pesticide of clove leaf extract gave better results in suppressing B. maydis. Similarly, in field testing, the application of B. subtilis formulation combined with clove leaf extract also gave a better effect in suppressing the development of maydis leaf blight disease and increasing yield. Applications of B. subtilis formulation can suppress maydis leaf blight disease by up to 13% and potentially increase yield by 26%.

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