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Daya Hambat Ekstrak Buah Okra (Abelmoschus esculentus L.) terhadap Pertumbuhan Candida albicans Iflachiana, Usykuri Naila; Dewi, Leni Rokhma; Prihanti, Ayu Mashartini
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 21 No 1 (2024)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v21i1.47338

Abstract

Oral candidiasis is a fungal infection of the superficial epithelium of the oral cavity caused by an overgrowth of Candida species. Effective therapy for oral candidiasis is nystatin. However, this drug has a variety of side effects. Therefore, natural therapeutic agent with minimal side effects is needed to be safe in long-term use. Okra fruit contains active ingredients that can be used as an antifungal agent. Aims to prove the ability of okra fruit extract and analyze the optimal concentration of okra fruit extract in inhibitting the growth of C. albicans. The antifungal activity test was carried out using the disk diffusion method. The research sample was divided into six groups, namely K (+) (nystatin), K (-) (DMSO 10%), and okra fruit extract with concentrations of 100%, 75%, 50%, and 25% obtained through the maceration process. Each research group was taken with a micropipette and then dripped on a paper disk. Paper disks were placed and distributed evenly on the surface of Saboroud Dextrose Agar (SDA) media that had been inoculated with C. albicans then incubated for 48 hours. The results showed the formation of a clear area around the paper disk that has been dropped with concentrations of 100%, 75%, and 50% of okra fruit extract. Okra fruit extract had an inhibitory effect on the growth of C. albicans and 100% concentration of okra fruit extract had the optimal ability to inhibit the growth of C. albicans.
The effectiveness of Edel varieties cacao leaf extract as an antibacterial against Staphylococcus aureus and Porphyromonas gingivalis: an experimental study Silvia, Rizka Maya; Viandisa, Chrysmyta Fiola; Prihanti, Ayu Mashartini; Wahyukundari, Melok Aris; Arina, Yuliana Mahdiyah Da'at
Padjadjaran Journal of Dentistry Vol 35, No 2 (2023): July 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no2.46171

Abstract

ABSTRACTIntroduction: Staphylococcus aureus (S. aureus) and Porphyromonas gingivalis (P. gingivalis) are the two main bacteria that cause dental and oral disease. Cacao leaves have been reported to have antibacterial properties. However, the active compounds in cacao leaves vary depending on the age of the leaves and the place where these plants grow. This research aims to analyze the active ingredients and antibacterial effectiveness of the Edel varieties cacao leaves against S. aureus and P. gingivalis. Methods: This is an experimental study. The Cacao leaves from PTPN 12 Plantation Banjarsari Jember were extracted by the maceration technique in concentrations of 25, 50, 75%, and 100%. The  phytochemical test was then made using the Thin Layer Chromatography (TLC). The antibacterial activity of Cacao leaf extract against S. aureus ATCC 25923 and P. gingivalis ATCC 33277 was examined using the Disc Diffusion technique on Mueller Hinton Agar (MHA) media. Results: The Cacao leaf extract of the Edel varieties from Jember contained flavonoids, saponins, and tannins but did not contain alkaloids. In all concentrations tested, the Cacao leafmextract of the Edel varieties from Jember showed the inhibition zones of S. aureus and P. gingivalis. The concentration of 100% showed the greatest antibacterial activity (The highest antibacterial activity was observed at a concentration of 100%), which was moderate against S. aureus (10.98 mm ± 0,93) and strong against P. gingivalis (11.54 mm ± 0,41). There was a significant difference among concentrations in both the P.gingivalis and S.aureus bacterial groups (p < 0.001). At the same concentration, there was no significant difference between P. gingivalis and S. aureus (p > 0.001). Conclusion: Cacao leaf  extract of the Edel varieties contains flavonoids, saponins and tannins and has antibacterial activity against S. aureus and P. gingivalis.