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All Journal Jurnal Fitopatologi Indonesia Plumula : Berkala Ilmiah Agroteknologi JUSTER: Jurnal Sains dan Terapan
Amara, Khansa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Energy Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics

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Composition of Functional Bacteria on Suppressive and Conducive Soil for Fusarium Wilt on Chilli Amara, Khansa; Giyanto; Widodo; Sudiana, I Made
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.215-229

Abstract

Composition of Functional Bacteria on Suppressive and Conducive Soil for Fusarium Wilt on Chilli The phenomenon of suppressive soil has been widely studied to control plant diseases in the field. Suppresive soil is characterized by its contain of functional microbes that can suppress pathogen populations. This study aims to determine and compare the composition of functional bacteria on suppressive and conducive soil of fusarium wilt. The research method consists of soil sampling in the field, isolation of functional bacteria from suppressive soil (SS) and conducive soil (CS) samples, calculation of bacterial population and type, biosafety screening, and characterization of functional bacteria in suppressing of Fusarium oxysporum. The results showed that the bacterial population in SS was higher than those in CS, especially for heat-tolerant bacteria and the fluorescent Pseudomonads. The high population of bacteria is supported by the high value of functional bacterial diversity in SS (H’ 3.70 > 3) compared to CS, which is classified as medium (1 < H’ 2.07 < 3), with the distribution of functional bacteria classified as almost evenly distributed and no dominance of certain types in SS and CS. The percentage of nonpathogenic bacteria in SS is higher than in CS, i.e. 51% and 23%, respectively. These bacteria have potential as plant growth promoting bacteria (PGPB) by producing IAA or dissolving phosphate alone of 24% and 10%, respectively in suppressive soils; and of 14% and 29%, respectively in conducive soils. Only bacteria derived from suppressive soils were able to produce IAA while dissolving phosphate with a percentage of as much as 48%.
Identifikasi dan Karakterisasi Bakteri Penyebab Hawar Daun Padi (Xanthomonas oryzae pv. oryzae) Af’idzatuttama, Af’idzatuttama; Anwar, Khairul; Amara, Khansa
JUSTER : Jurnal Sains dan Terapan Vol. 4 No. 2 (2025): JUSTER: Jurnal Sains dan Terapan
Publisher : Jompa Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57218/juster.v4i2.1786

Abstract

Salah satu penyakit penting yang menyerang tanaman padi yaitu Hawar Daun Bakteri (HDB). Penyakit HDB ini disebabkan oleh bakteri Xanthomonas oryzae pv. oryzae (Xoo). Gejala khas penyakit ini yaitu gejala diawali dari pucuk daun yang berwarna keabu-abuan berupa bercak pada satu atau kedua sisi daun, bercak akan meluas kearah pangkal daun dan melebar sehingga terbentuk hawar (blight). Isolasi bakteri Xoo dilakukan berdasarkan tanaman padi yang terserang hawar daun di lapang. Sampel diambil dari 4 Provinsi yaitu Jawa Tengah, Jawa Barat, Jawa Timur, dan Sumatera Selatan. Masing-masing sampel daun padi diisolasi pada media spesifik Xoo yaitu Xanthomonas Agar (XA). Identifikasi X. oryzae pv. oryzae dilakukan berdasarkan pada uji sifat Gram, uji hipersensitivitas, uji Starch Hydrolysis, dan uji patogenesitas dengan tanaman padi. Hasil pengujian menunjukkan bakteri Xanthomonas oryzae bersifat Gram negatif, uji hipersensitivitas positif, tidak menunjukkan zona bening di area koloni pada uji Starch Hydrolysis, dan menunjukkan gejala lesion pada uji patogenesitas. Koloni bakteri hasil identifikasi Xanthomonas oryzae pv. oryzae (Xoo) berwarna kuning cerah, berlendir, bulat, dan tepian rata.
Potensi Trichoderma spp. dari Koleksi Tanah Tanaman Perkebunan asal Jawa Timur sebagai Antagonis Fusarium oxysporum Amara, Khansa; Indrawan, Arga Dwi; Af’idzatuttama
Plumula : Berkala Ilmiah Agroteknologi Vol. 13 No. 2: Juli 2025
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v13i2.261

Abstract

Aplikasi Trichoderma spp. hasil eksplorasi pada tanah tanaman sehat dapat berpotensi dalam pengendalian penyakit layu yang ramah lingkungan dan dapat diterapkan dalam jangka panjang. Penelitian ini bertujuan untuk menilai kualitas spora dan kemampuan antagonis Trichoderma spp. yang diisolasi dari tanah tanaman kakao, kopi, dan cengkeh terhadap F. oxysporum secara in vitro. Penelitian disusun dengan rancangan acak lengkap (RAL), dengan perlakuan jenis Trichoderma dari sumber sampel tanah berbeda. Variabel pengamatan meliputi: kerapatan dan viabilitas spora, serta tingkat hambatan relatif yang selanjutnya dianalisis ragam (ANOVA) menggunakan SAS on Demand, dan uji lanjut Tukey pada taraf 5%. Seluruh isolat Trichoderma spp. menunjukkan hasil melebihi kriteria penilaian standar mutu sebagai agens hayati berdasarkan SNI 8027.3:2014, dengan kerapatan spora 108 spora/ml. Namun, hanya isolat Trichoderma spp. asal tanah tanaman kopi yang memiliki kualitas spora dan persentase penghambatan terbaik dalam menekan pertumbuhan diameter koloni F. oxysporum, yaitu sebesar 59%.
Co-Authors Af’idzatuttama Af’idzatuttama, Af’idzatuttama Giyanto I Made Sudiana Indrawan, Arga Dwi Khairul Anwar Widodo