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Isroni, Muhson
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Aktivitas Bakteri Kitinolitik dari Cangkang Perna viridis sebagai Antifungi Phytophthora palmivora Penyebab Penyakit Busuk Buah Kakao (Theobroma cacao L.) Agustin, Debby; Khikmah, Ulfia Nurul; Isroni, Muhson; Maulidiya, Anisa
bionature Vol 18, No 2 (2017): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.543 KB) | DOI: 10.35580/bionature.v18i2.6139

Abstract

Abstract. The emphasis on the growth of Phytophthora palmivora was important in order to reduce Pod Rot Disease of cacao (Theobrorna cacao L.) which could harm agriculture sector. Some bacteria had chitinolytic enzyme activity that is potentially used as an antifungal against Phytophthora palmivora, because the cell wall of the fungi composed of chitin. The purpose of this research was to know chitinolytic bacteria from Perna viridis shell which had higher activity of chitinase enzyme, the amount of chitinase enzyme activity of each selected isolate, and to know the effect of chitinolytic bacterial isolates from Perna viridis shell to reduce the growth of Phytophthora palmivora. The bacteria were isolated from Perna viridis shell at Depok Beach, Kretek, Bantul, Yogyakarta. This research was an explorative research which include bacterial characterization and experimental research which include antagonistic test of chitinolytic bacteria against Phytophthora palmivora. The chitinolytic bacteria was isolated using selective chitin agar medium by pour plate method and then screening the isolates that had chitinase enzyme activity by measuring the enzyme activity of each bacterial isolates by spectrophotometric method. Selected bacterial isolates were characterized by macroscopic, microscopic and physiological characters. The bacteria that had been selected tested for their ability to reduce the growth of Phytophthora palmivora by Kirby Bauer modification method. The result showed that there were 10 isolates that had chitinase enzyme activity which two selected isolates had the higher chitinase enzyme activity. There were 7D and 6B isolates. The isolate 7D had 1,258 u/ml chitinase enzyme activity and isolate 6B had 1,212 u/ml chitinase enzyme activity. The result of chitinolytic bacterial antagonist test on Phytophthora palmivora growth showed that both bacterial isolates were potential to antifungal Phytophthora palmivora and showed a real effect in inhibiting the growth of Phytophthora palmivora with significance value < 0,05.Keywords: Chitinolytic Bacteria, Perna viridis, Phytophthora palmivora
Aktivitas Bakteri Kitinolitik dari Cangkang Perna viridis sebagai Antifungi Phytophthora palmivora Penyebab Penyakit Busuk Buah Kakao (Theobrorna cacao L.) Agustin, Debby; Khikmah, Ulfia Nurul; Isroni, Muhson; Maulidiya, Anisa
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 14, No 1 (2017): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The emphasis on the growth of Phytophthora palmivora was important in order to reduce Pod Rot Disease of cacao (Theobrorna cacao L.) which could harm agriculture sector. Some bacteria had chitinolytic enzyme activity that is potentially used as an antifungal against Phytophthora palmivora, because the cell wall of the fungi composed of chitin. The purpose of this research were to know chitinolytic bacteria from Perna viridis shell which had higher activity of chitinase enzyme, the amount of chitinase enzyme activity of each selected isolate, and to know the effect of chitinolytic bacterial isolates from Perna viridis shell to reduce the growth of Phytophthora palmivora. The bacteria were isolated from Perna viridis shell at Depok Beach, Kretek, Bantul, Yogyakarta. This research were an explorative research which include bacterial characterization and experimental research which include antagonistic test of chitinolytic bacteria against Phytophthora palmivora. The chitinolytic bacteria was isolated using selective chitin agar medium by pour plate method and then screening the isolates that had chitinase enzyme activity by measuring the enzyme activity of each bacterial isolates by spectrophotometric method. Selected bacterial isolates were characterized by macroscopic, microscopic and physiological characters. The bacteria that had been selected tested for their ability to reduce the growth of Phytophthora palmivora by Kirby Bauer modification method. The result showed that there were 10 isolates that had chitinase enzyme activity which two selected isolates had the higher chitinase enzyme activity. There were 7D and 6B isolates. The isolate 7D had 1,258 u/ml chitinase enzyme activity and isolate 6B had 1,212 u/ml chitinase enzyme activity. The result of chitinolytic bacterial antagonist test on Phytophthora palmivora growth showed that both bacterial isolates were potential to antifungal Phytophthora palmivora and showed a real effect in inhibiting the growth of Phytophthora palmivora with significance value < 0,05.
PotensiEkstrakKulitPisang (Musa Paradisiaca) SebagaiAntibakteri Ralstonia solanacearum Penyebab Penyakit Layu Bakteri Tanaman Tomat Isroni, Muhson; Paskariani, Angela Enggar; Setiawan, Fauzi Fandy; Rakhmawati, Anna
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

Indonesia is one of the largest banana-producing countries in the world. Unfortunately, the high demand for bananas has not been balanced with the utilization of banana peels well. Thus, the high production of banana indicates the high amount of banana peel waste produced. On the other hand, tomato production from year to year tends to decrease. According to the Director General of Holticulture (2015), tomato production in 2014 decreased by 76.793 tons. One of the causes is the attack of Ralstoniasolanacearum bacteria that causes bacterial wilt diseases of tomato plants. Vegetable pesticides can come from banana peels because they contain toxin compounds such as flavonooids, sapoin, tannins, etc. Flavonoids will form complex compounds with extracellular and dissolved proteins that can damage the cell membrane of bacteria and followed by the release of intracellular compounds. This study aims to know the process of banana peel extract (Musa paradisiaca) in suppressing the growth of Ralstoniasolanacearum bacteria, which is most effective for inhibiting the growth of Ralstoniasolanacearum bacteria, This research is a  experimental research. the experiment is antibacterial test of banana peel extract on Ralstoniasolanacearum bacteria growth. The design pattern of the study used a completely nested randomized design pattern. The research started with the extraction process with maceration, making of NA and NB media, followed by rejuvenation and suspension of bacteria. After that, making test solution, then test the antibacterial activity with kirby-bauer method and last data retrieval. The results of Antibacterial test results showed positive results can inhibit the growth of Ralstoniasolanacearum bacteria is evidenced by ANOVA of significan value less than 0.05. The Duncan test results show that 100% of Ammon banana is the best to be used for antibacterial. So that banana peel potency to be developed become agent of vegetable pesticide on tomato plant.
Co-Authors Agustin, Debby Anna Rakhmawati Khikmah, Ulfia Nurul Maulidiya, Anisa Paskariani, Angela Enggar Setiawan, Fauzi Fandy