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All Journal Jurnal Penelitian Saintek Jurnal Ilmu Lingkungan Jurnal Penelitian dan Pengembangan Pendidikan Jurnal Sains Dasar Journal of Degraded and Mining Lands Management Jurnal Penelitian Pendidikan IPA (JPPIPA) ALCHEMY Jurnal Penelitian Kimia Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Molekul: Jurnal Ilmiah Kimia Journal of Innovation in Educational and Cultural Research JIPB (Jurnal Inovasi Pembelajaran Biologi) Proceeding Biology Education Conference Kaunia: Integration and Interconnection of Islam and Science Journal Research and Development in Education (RaDEn) Jurnal Natural Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Jurnal Edukasi Biologi Kingdom (The Journal of Biological Studies) Quagga: Jurnal Pendidikan dan Biologi Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi
Anna Rakhmawati
Universitas Negeri Yogyakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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THE OPTIMATION OF CELLULASE ENZYME OF MOLD ISOLATED FROM AGRICULTURE LAND IN WUKIRSARI AFTER MERAPI ERUPTION Umniyatie, Siti; Rakhmawati, Anna; Yulianti, Evy
Jurnal Sains Dasar Vol 4, No 1 (2015): April 2015
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.835 KB) | DOI: 10.21831/jsd.v4i1.8445

Abstract

The aim of this study was to develop potency of mold isolated from agriculture land after Merapi eruption in 2010 at Wukirsari, Cangkringan, Sleman, Yogyakaerta. The treatment were temperature and pH optimation to the cellulase enzyme activity and the protein level yield by mold isolate A 2.10, A 2.15 and B 3.18 in different substrate, avicel and carboxymethyl cellulose (CMC). This study was experimental study to find out the substrate, pH and temperature which yield the best activity and protein level from mold isolate A 2.10, A 2.15 dan B 3.18. the population of this study all of the crude enzymes from mold isolate, and the sample was 1 ml of crude enzyme which treated with different substrate, pH and temperature. The result of this study showed that the best susbtrate was avicel and the optimum temeperature and pH for isolate A.2.10 was 25 ⁰C Keywords: optimation, cellulase enzyme, cellulolytic
PotensiEkstrakKulitPisang (Musa Paradisiaca) SebagaiAntibakteri Ralstonia solanacearum Penyebab Penyakit Layu Bakteri Tanaman Tomat Isroni, Muhson; Paskariani, Angela Enggar; Setiawan, Fauzi Fandy; Rakhmawati, Anna
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indonesia is one of the largest banana-producing countries in the world. Unfortunately, the high demand for bananas has not been balanced with the utilization of banana peels well. Thus, the high production of banana indicates the high amount of banana peel waste produced. On the other hand, tomato production from year to year tends to decrease. According to the Director General of Holticulture (2015), tomato production in 2014 decreased by 76.793 tons. One of the causes is the attack of Ralstoniasolanacearum bacteria that causes bacterial wilt diseases of tomato plants. Vegetable pesticides can come from banana peels because they contain toxin compounds such as flavonooids, sapoin, tannins, etc. Flavonoids will form complex compounds with extracellular and dissolved proteins that can damage the cell membrane of bacteria and followed by the release of intracellular compounds. This study aims to know the process of banana peel extract (Musa paradisiaca) in suppressing the growth of Ralstoniasolanacearum bacteria, which is most effective for inhibiting the growth of Ralstoniasolanacearum bacteria, This research is a  experimental research. the experiment is antibacterial test of banana peel extract on Ralstoniasolanacearum bacteria growth. The design pattern of the study used a completely nested randomized design pattern. The research started with the extraction process with maceration, making of NA and NB media, followed by rejuvenation and suspension of bacteria. After that, making test solution, then test the antibacterial activity with kirby-bauer method and last data retrieval. The results of Antibacterial test results showed positive results can inhibit the growth of Ralstoniasolanacearum bacteria is evidenced by ANOVA of significan value less than 0.05. The Duncan test results show that 100% of Ammon banana is the best to be used for antibacterial. So that banana peel potency to be developed become agent of vegetable pesticide on tomato plant.
EXPLORATION OF THERMOFILIC BACTERIA POST ERUPSI MERAPI AS EXTRACELULAR ENZYME MANUFACTURER Anna Rakhmawati; Evy Yulianti
Jurnal Penelitian Saintek Vol 17, No 1: April 2012
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8004.652 KB) | DOI: 10.21831/jps.v17i1.1741

Abstract

Penelitian mengenai bakteri termofilik pasca erupsi Merapi masih sangat terbatas padahal masih banyak potensi yang dapat diteliti, salah satunya mengenai enzim ekstraseluler yang dihasilkannya. Tujuan penelitian ini adalah untuk isolasi, karakterisasi dan memperoleh isolat bakteri termofilik yang mampu menghasilkan enzim ekstraseluler. Isolasi bakteri termofilik dilakukan dengan menggunakan 2 (dua) metode yaitu dilution dan enrichment dengan media Nutrient Broth kemudian diinkubasi pada 55 ºC, dilanjutkan dengan seleksi pada suhu 70 ºC. Karakterisasi yang dilakukan, yaitu karakterisasi morfologi koloni. Setelah itu dilakukan skrining aktivitas enzim amilase, protease, dan selulase. Hasil penelitian menunjukkan bakteri termofilik pasca erupsi Merapi yang diisolasi dari sampel air dan pasir Kali Gendol Atas dengan suhu inkubasi 55⁰C diperoleh 480 isolat, setelah diseleksi pada suhu 70 ⁰C diperoleh 253 isolat. Karakter fenotipik isolat bakteri termofilik pasca erupsi Merapi menunjukkan keanekaragaman morfologi koloni meliputi warna, bentuk, ukuran, tepi, dan elevasi koloni. Isolat bakteri termofilik yang menghasilkan enzim ekstraseluler amilase sebanyak 9 isolat, enzim protease sebanyak 4 isolat, dan 1 isolat penghasil enzim selulase pada suhu inkubasi 70 ⁰C .Kata kunci: bakteri, termofilik, enzim ekstraseluler
OPTIMATION OF ANTIMICROBIAL SUBSTANCE PRODUCTION IN CELL FREE EXTRACT FROM THERMOPHYLIC BACTERIA FERMENTATION AFTER MERAPI ERRUPTION Evy Yulianti; Anna Rakhmawati; Kartika Ratna Pertiwi
Jurnal Sains Dasar Vol 4, No 2 (2015): October 2015
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.196 KB) | DOI: 10.21831/jsd.v4i2.9089

Abstract

The aims of this study was to investigate the effect of medium with different pH (6,7,9), salt concentration (0,5; 1; 2 %) and fermentation periode (24 and 48 hr) to the antimicrobial activity of cell free extract to three pathogens, Eschericia coli, Staphylococcus aureus, and Candida albican. This study consists of antimicrobial compounds production with different medium and continued by antimicrobial tested to fungi and bacterial pathogens Eschericia coli, Staphylococcus aureus, and Candida albican by Kirby Bauer  method with paper disk. Different salt concentration, pH and fermentation periode affected the  antimicrobial activity potency of cell free extract yielded by thermophylic bacteria. Treatment which yielded CFE with the best antimicrobial activity was treatment with 24 hr fermentation, pH 7 and salt concentration 2% to S aureus and pH 6 salt concentration 1% to E coli. Cell free extract had no potency as antifungi to Candida albicans except CFE yielded by thermophylic bacteria fermented in medium with pH 7 and salt concentration 1% in 24 hr with inhibition zone index 1,17. Keywords: cell free extract, antimicrobial, pH, salt concentration, fermentation period
Isolasi dan uji aktivitas enzim lipase termostabil dari bakteri termofilik pasca erupsi Merapi Drajat Pramiadi; Evy Yulianti; Anna Rakhmawati
Jurnal Sains Dasar Vol 3, No 1 (2014): April 2014
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.462 KB) | DOI: 10.21831/jsd.v3i1.2780

Abstract

The usage of enzyme which comes from microorganism is more advantageous because it is easier to be multiplied in a relatively short time and does not need a large space, including lipase enzyme, which is often used in industries. The objective of this research is to isolate thermo stable lipase enzyme from thermophilic bacteria and to understand specific ativities. The thermophilic bacteria isolation is done using pour plate method. Selection of the bacteria producing lipase is shown from the presence of the biggest translucent zone around the colony on the olive oil medium with 0.1% Rhodamine-B. Two isolate produced by the biggest translucent zone are then continued by lipase enzyme isolation and activity tests. From 253 isolate, 43 isolate can grow on the medium that contains olive oil and 2 isolate shows translucent zone around the isolate, that is isolate 361 and isolate 213. The growth curve measurement for 24 hours shows that the exponential time is reached at the 10th hour for both isolates. The result of this research shows that temperature, pH, and enzyme isolate variation effects the enzyme activities. The enzyme activity tests show that on isolate 361, the best enzyme activities is obtained from incubation temperature of 50⁰C with pH 7, whereas for isolate 213, the best enzyme activities is reached when incubation temperature is 70⁰C with pH 6.   Key words: lipase, olive oil, thermophilic bacteria, thermo stable enzyme, enzyme activities
UTILIZATION PROBIOTIC LACTIC ACID BACTERIA FISH FROM WASTE FAECES ON LDL LEVEL BLOOD BROILER CHICKENS STRAIN LOHMANN Astuti Astuti; Siti Umniyati; Anna Rakhmawati; Evy Yulianti
Jurnal Sains Dasar Vol 5, No 1 (2016): April 2016
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.315 KB) | DOI: 10.21831/jsd.v5i1.12665

Abstract

Abstract This study aims to determine the effect of lactic acid bacteria supplement from sewge force feeding fish to the LDL content of broiler chicken blood. The research samples are 40 of 1 day old broiler chicken from PT. Multi Breeder Adiram, which were divided into 4 treatments, were taken randomly and maintined for 42 days. Data were analyzed by analysis of variance using a completely randomized design unidirectional pattern, followed by a test of Duncan's Multiple Range Test (DMRT).Probiotic Lactic Acid Bacteria Isolates treatment (BAL) were used in this study is the bacterium Streptococcus thermopillus in the form of freeze drying from the Laboratory of Nutritional Biochemistry, Faculty of Animal Husbandry, UGM. I as a control treatment (without BAL) Treatment II BAL cell count was 106 CFU / ml., The third treatment is the number of BAL Cells are 107CFU / ml. , IV treatment BAL cell count was 108 CFU /ml. The results showed that there is no significant effect of BAL isolate addition on the LDL level of the broiler chicken blood. Keywords: lactic acid bacteria, LDL, freeze drying
KEANEKARAGAMAN ORGANISME PADA RHIZOSFER GULMA SIAM DI LAHAN VULKANIK, PESISIR, DAN KARST Tien Aminatun; Siti Umniyatie; Anna Rakhmawati; Aji Suhandy; Nrangwesthi Widyaningrum; Kurnia Cahyani
Jurnal Penelitian Saintek Vol 26, No 1 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i1.38754

Abstract

Penelitian ini bertujuan untuk membandingkan kondisi edafik, hubungan antara kondisi edafik dan keanekaragaman organisme tanah, serta perbedaan keanekaragaman organisme tanah pada berbagai ekosistem rhizosfer gulma Siam (Chromolaena odorata) yang tumbuh di lahan vulkanik, pesisir, dan karst. Penelitian dilakukan dengan mengambil sampel tanah dari ekosistem rhizosfer gulma Siam di lahan vulkanik, karst, dan pesisir Daerah Istimewa Yogyakarta; mengamati komposisi organisme tanah di laboratorium, yaitu collembola, nematoda, dan mikoriza. Hasil penelitian menunjukkan bahwa tanah pada rhizosfer gulma Siam yang tumbuh di lahan karst menunjukkan tingkat kesuburan tertinggi yang ditunjukkan oleh kandungan N, K, dan C-organik tanah, sedangkan tanah pesisir memiliki kandungan P tertinggi. Tekstur tanah pada rhizosfer gulma Siam lebih berpengaruh terhadap keanekaragaman organisme tanah dibandingkan kandungan hara tanah dan sifat tanah lainnya yang diteliti. Keragaman organisme tanah rhizosfer gulma Siam lebih besar di lahan pesisir dibandingkan di lahan karst dan vulkanik. Implikasi dari hasil tersebut adalah bahwa tanah dengan tekstur berpasir akan lebih bermanfaat bagi pertumbuhan gulma Siam dengan keanekaragaman organisme rhizosfer yang lebih tinggi.ORGANISM DIVERSITY IN THE RHIZOSPHERE OF SIAM WEEDS IN VOLCANIC, COASTAL AND KARST LANDThe research aims to compare the edaphic condition, the relationship between the edaphic condition and soil organism diversity, and the differences of soil organism diversity in different rhizosphere ecosystems of Siam weed growing in volcanic, coastal and karts areas. The research was conducted by taking soil samples from the Siam weed rhizosphere ecosystems in volcanic, karst and coastal areas of the Special Region of Yogyakarta and observing the composition of the soil organisms in the laboratory, including collembola, nematode, and mycorrhiza. The results find that soil from rhizosphere of Siam weed growing in karst area shows the highest level of fertility indicated by the N, K, and C-organic contents of the soil, whereas soil from coastal area has the highest P content. Soil texture in the rhizosphere of Siam weed has more effect on the diversity of soil organisms than the soil nutrient content and other soil properties investigated. The diversity of soil organisms of Siam weed rhizosphere is greater in coastal area than those in karst and volcanic areas. The implication of the results is that soil with a sandy texture will be more beneficial for the growth of Siam weed with a higher diversity of rhizosphere organisms
SELEKSI BAKTERI TERMOFILIK SELULOLITIK PASCA ERUPSI MERAPI Anna Rakhmawati; Evy Yulianti; Eli Rohaeti
Kaunia: Integration and Interconnection Islam and Science Vol. 10 No. 2 (2014)
Publisher : Fakultas Sains dan Teknologi UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/kaunia.1070

Abstract

Thermophilic bacteria after the eruption of Merapi in 2010 have been isolated from the Gendol Atas river with dilution and enrichment methods. The purpose of this study was to conduct the selection of these bacteria as producers of cellulase enzymes. Selection was done by growing 348 bacterial isolates on selective media Mandels-CMC 0.5% using Carboxy Methyl Cellulose (CMC) as carbon source. Incubation was performed at 55 °C for 24 hours. The results showed 255 isolates (73.27%) were able to grow in Mandels medium-CMC 0.5%. Bacterial isolates were isolated by enrichment method (69.8%) were more able to grow on selective media compared with the dilution method (30.2%). The diameter of the three largest colonies were isolates D13a (2.44 cm); E135 (2.33 cm); and D110a (1.98 cm).
BACTERIAL CELLULOSE FROM RICE WASTE WATER AND ITS COMPOSITE WHICH ARE DEPOSITED NANOPARTICLE AS AN ANTIMICROBIAL MATERIAL Eli Rohaeti; Endang W Laksono; Anna Rakhmawati
ALCHEMY Jurnal Penelitian Kimia Vol 12, No 1 (2016): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.12.1.946.70-87

Abstract

Bacterial cellulose (C) and its composites were synthesized from rice waste water with addition of glycerol (G) and chitosan (Ch).Antibacterial activity of the C, the bacterial cellulose-chitosan composite (CCh), and the bacterial  cellulose – glycerol - chitosan composite (CGCh) which were deposited silver nanoparticles against S. aureus, E. coli,  and yeast C. albicans has been conducted. Silver nanoparticles was prepared by chemical reduction of a silver nitrate solution, a trisodium citrate as a reductor, and a PVA as a stabilizer. The UV-Vis spectroscopy is used to determine the formation of silver nanoparticles. The characterization was conducted on the bacterial celluloses and those composites including the functional groups by the FTIR, the mechanical properties by Tensile Tester, photos surfaces by SEM, and the test of the antibacterial activity against S. aureus, E. coli, and C. albicans by diffusion method. The silver nanoparticle characterization indicates that the silver nanoparticles are formed at a wavelength of 418.80 nm. The antibacterial test showed an inhibitory effect of the C, the CCh, and the CGCh which are deposited  the silver nanoparticles against of S. aureus, E. coli,  and C.albicans. The CGChs which are deposited silver nanoparticles has the highest antimicrobial activity against the Staphylococcus aureus ATCC 25923. The CGs which are deposited silver nanoparticles provide the highest antimicrobial activity against the E. coli ATCC 25922 and the yeast Candida albicans ATCC 10231.
Pelatihan Identifikasi Potensi Hazard Bahan Pangan Sebagai Upaya Pencegahan Keracunan Jajanan Anak Sekolah Anna Rakhmawati; Siti Umniyatie; Evy Yulianti
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 1, No 2 (2017)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.459 KB) | DOI: 10.21831/jpmmp.v1i2.15561

Abstract

Kasus keracunan karena mengkonsumsi jajanan anak sekolah masih banyak dijumpai di tingkat sekolah dasar. Target pelaksanaan Program Pengabdian  kepada Masyarakat yaitu terjadinya peningkatan pengetahuan dan keterampilan guru dan karyawan SD Muhammadiyah Sleman  dalam mengenali dan mengidentifikasi potensi hazard (cemaran) bahan pangan; meningkatkan keterampilan dalam memilih bahan pangan yang aman pada jajanan anak sekolah. Kemudian mendampingi dan memotivasi kader guru untuk mengimplementasikan bekal pengetahuan dan keterampilan yang sudah diperoleh dalam mencegah keracunan jajanan anak sehingga jajanan yang dikonsumsi bergizi seimbang, sehat dan higienis. Tahap persiapan meliputi audiensi, koordinasi, dan pemantapan program dengan mitra kemudian identifikasi peserta kegiatan. Tahap pelaksanaan yaitu kegiatan seminar berupa pemberian materi dan pelatihan.  Materi disampaikan oleh tim pengabdi dilanjutkan  pelatihan keterampilan mengkarakterisasi potensi cemaran baik fisik, kemis maupun biologis pada jajanan anak serta upaya pencegahannya. Kegiatan terakhir yaitu evaluasi dan perbaikan. Hasil program menunjukkan keberhasilan proses yang ditunjukkan dengan kehadiran peserta mencapai 100%, peningkatan hasil tes pengetahuan sebesar  20,47 %, dan sebanyak 90% peserta telah memiliki keterampilan dalam memilih bahan pangan yang aman pada jajanan anak sekolah. 
Co-Authors Abdullah Nashih Amin Ajeng Septiana Putri Aji Suhandy Althaaf, Nabila Tsabita Arifudin Idrus Astuti Astuti Aulia, Fera Ayu Natasya FR BAHARUDIN, JALU BAHTIAR Baso Samsu Rijal Bernadetta Octavia Bidhari Pidhatika Budiwati Budiwati David Meilana Nur Arifin Dhiya Millatika Tohiri Drajat Pramiadi Dwi Ratnawati, Dwi Efendi, Adhan Ega Safitri Eli Rohaeti Endang W Laksono Endang WLFX Eny Purwanti Evy Yulianti Ferella*, Elsa Fitri Rahmawati Hajidah Musyayyadah Hanifa Rizky Rahmawati Intan Lestari Isroni, Muhson Istiana Prihatini Kartika Ratna Pertiwi Kun Sri Budiasih Kurnia Cahyani Lili Sugiyarto Lili Sugiyarto Linda, Evi Marfuatun, Marfuatun Muhammad Anees Novian Indah Sari, Tasya Nrangwesthi Widyaningrum Nunuk Purwanti Nur Fathurahman Ridwan Nur Rizki Putri Ramadhanti Paidi Paskariani, Angela Enggar Pertiwi, Melania Citra Pramesty, Alfinda Ratnasari, Tantri Ayu Ria Dewi Dili Hastuti Ria Dewi Dili Hastuti Rindiana, Danny Ria Rizka Apriani Putri Sajid Iqbal Sari, Tasya Novian Indah Setiawan, Fauzi Fandy Simbolon, Doly Siti Umniyati Siti Umniyatie Siti Umniyatie Siti Umniyatie SRI ATUN Suhartini Suhartini Suhartini Suhartini Suhartini Sujangka, Arsyad Tasya Novian Indah Sari Tien Aminatun Tutiek Rahayu Wahyu Nuryadi H Yulianti Yulianti Yuliati Yuliati Yuni Wibowo